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Originally published in Science Express on 24 January 2002
Science 22 February 2002:
Vol. 295. no. 5559, pp. 1523 - 1525
DOI: 10.1126/science.1067861
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Reports

Role of Nucleoporin Induction in Releasing an mRNA Nuclear Export Block

Jost Enninga,1 David E. Levy,2 Günter Blobel,1* Beatriz M. A. Fontoura13

Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC). Some NPC components, such as the nucleoporins (Nups) Nup98 and Nup96, are also associated with the nuclear interior. Nup98 is a target of the vesicular stomatitis virus (VSV) matrix (M) protein-mediated inhibition of messenger RNA (mRNA) nuclear export. Here, Nup98 and Nup96 were found to be up-regulated by interferon (IFN). M protein-mediated inhibition of mRNA nuclear export was reversed when cells were treated with IFN-gamma or transfected with a complementary DNA (cDNA) encoding Nup98 and Nup96. Thus, increased Nup98 and Nup96 expression constitutes an IFN-mediated mechanism that reverses M protein-mediated inhibition of gene expression.

1 Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA.
2 Department of Pathology and Kaplan Comprehensive Cancer Center, New York University School of Medicine, New York, NY 10016, USA.
3 Department of Molecular and Cellular Pharmacology and Sylvester Cancer Center, University of Miami School of Medicine, Miami, FL 33136, USA.
*   To whom correspondence should be addressed. E-mail: blobel{at}rockvax.rockefeller.edu

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