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Science 25 January 2002:
Vol. 295. no. 5555, pp. 694 - 697
DOI: 10.1126/science.1067534
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Reports

RNA Helicase MUT-14-Dependent Gene Silencing Triggered in C. elegans by Short Antisense RNAs

Marcel Tijsterman,* René F. Ketting,* Kristy L. Okihara, Titia Sijen, Ronald H. A. Plasterkdagger

Posttranscriptional gene silencing in Caenorhabditis elegans results from exposure to double-stranded RNA (dsRNA), a phenomenon designated as RNA interference (RNAi), or from co-suppression, in which transgenic DNA leads to silencing of both the transgene and the endogenous gene. Here we show that single-stranded RNA oligomers of antisense polarity can also be potent inducers of gene silencing. As is the case for co-suppression, antisense RNAs act independently of the RNAi genes rde-1 and rde-4 but require the mutator/RNAi gene mut-7 and a putative DEAD box RNA helicase, mut-14. Our data favor the hypothesis that gene silencing is accomplished by RNA primer extension using the mRNA as template, leading to dsRNA that is subsequently degraded.

Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed at Hubrecht Laboratory, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands. E-mail: plasterk{at}niob.knaw.nl

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