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Science 11 January 2002:
Vol. 295. no. 5553, pp. 342 - 345
DOI: 10.1126/science.1064938
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Reports

Stage-Specific Transcription of Distinct Repertoires of a Multigene Family During Plasmodium Life Cycle

P. R. Preiser,1* S. Khan,1 F. T. M. Costa,2 W. Jarra,1 E. Belnoue,2 S. Ogun,1 A. A. Holder,1 T. Voza,3 I. Landau,3 G. Snounou,4 L. Rénia2

Members of a multigene family in the rodent malaria parasite Plasmodium yoelii yoelii code for 235-kilodalton proteins (Py235) that are located in the merozoite apical complex, are implicated in virulence, and may determine red blood cell specificity. We show that distinct subsets of py235 genes are expressed in sporozoites and hepatic and erythrocytic stages. Antibodies to Py235 inhibited sporozoite invasion of hepatocytes. The switch in expression profile occurred immediately after transition from one stage to another. The results suggest that this differential expression is driven by strong biological requirements and provide evidence that hepatic and erythrocytic merozoites differ.

1 Division of Parasitology, National Institute for Medical Research, The Ridgeway, London, NW7 1AA, UK.
2 INSERM U445, Département d'Immunologie, Institut Cochin de Génétique Moléculaire, Hôpital Cochin, Université René Descartes, Bâtiment G. Roussy, 27 Rue du Faubourg Saint-Jacques, 75014 Paris, France.
3 Laboratoire de Biologie Parasitaire, Muséum National d'Histoire Naturelle, 61 Rue Buffon, 75231 Paris Cedex 05, France.
4 Unité de Parasitologie Biomédicale, Institut Pasteur, 25 and 28 Rue du Dr. Roux, 75724 Paris Cedex 15, France.
*   To whom correspondence should be addressed. E-mail: ppreise{at}nimr.mrc.ac.uk

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Distinct Trafficking and Localization of STEVOR Proteins in Three Stages of the Plasmodium falciparum Life Cycle.
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Cellular Gene Expression upon Human Immunodeficiency Virus Type 1 Infection of CD4+-T-Cell Lines.
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Polymorphism in a Plasmodium falciparum Erythrocyte-binding Ligand Changes Its Receptor Specificity.
D.C. G. Mayer, J.-B. Mu, X. Feng, X.-z. Su, and L. H. Miller (2002)
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Small Variant STEVOR Antigen Is Uniquely Located within Maurer's Clefts in Plasmodium falciparum-Infected Red Blood Cells.
M. Kaviratne, S. M. Khan, W. Jarra, and P. R. Preiser (2002)
Eukaryot. Cell 1, 926-935
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