Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Applied Biosystems siRNA

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 21 December 2001:
Vol. 294. no. 5551, pp. 2546 - 2549
DOI: 10.1126/science.1066700
Prev | Table of Contents | Next

Reports

Control of Stem Cell Self-Renewal in Drosophila Spermatogenesis by JAK-STAT Signaling

Natalia Tulina, Erika Matunis*

Stem cells, which regenerate tissue by producing differentiating cells, also produce cells that renew the stem cell population. Signals from regulatory microenvironments (niches) are thought to cause stem cells to retain self-renewing potential. However, the molecular characterization of niches remains an important goal. In Drosophila testes, germ line and somatic stem cells attach to a cluster of support cells called the hub. The hub specifically expresses Unpaired, a ligand activating the JAK-STAT (Janus kinase-signal transducer and activator of transcription) signaling cascade. Without JAK-STAT signaling, germ line stem cells differentiate but do not self-renew. Conversely, ectopic JAK-STAT signaling greatly expands both stem cell populations. We conclude that the support cells of the hub signal to adjacent stem cells by activation of the JAK-STAT pathway, thereby defining a niche for stem cell self-renewal.

Department of Embryology, Carnegie Institution of Washington, 115 West University Parkway, Baltimore, MD 21210, USA.
*   To whom correspondence should be addressed. E-mail: matunis{at}ciwemb.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Seminal discoveries in regenerative medicine: contributions of the male germ line to understanding pluripotency.
N. Geijsen and D. L. Jones (2008)
Hum. Mol. Genet. 17, R16-R22
   Abstract »    Full Text »    PDF »
Drosophila optic lobe neuroblasts triggered by a wave of proneural gene expression that is negatively regulated by JAK/STAT.
T. Yasugi, D. Umetsu, S. Murakami, M. Sato, and T. Tabata (2008)
Development 135, 1471-1480
   Abstract »    Full Text »    PDF »
Stem Cell Niche: Microenvironment and Beyond.
J. Zhang and L. Li (2008)
J. Biol. Chem. 283, 9499-9503
   Full Text »    PDF »
Lessons Learned About Adult Kidney Stem Cells From the Malpighian Tubules of Drosophila.
S. R. Singh and S. X. Hou (2008)
J. Am. Soc. Nephrol. 19, 660-666
   Abstract »    Full Text »    PDF »
The JAK/STAT pathway positively regulates DPP signaling in the Drosophila germline stem cell niche.
L. Wang, Z. Li, and Y. Cai (2008)
J. Cell Biol. 180, 721-728
   Abstract »    Full Text »    PDF »
Jak/Stat signalling in niche support cells regulates dpp transcription to control germline stem cell maintenance in the Drosophila ovary.
L. Lopez-Onieva, A. Fernandez-Minan, and A. Gonzalez-Reyes (2008)
Development 135, 533-540
   Abstract »    Full Text »    PDF »
Asymmetric centrosome behavior and the mechanisms of stem cell division.
Y. M. Yamashita and M. T. Fuller (2008)
J. Cell Biol. 180, 261-266
   Abstract »    Full Text »    PDF »
The hematopoietic stem cell and its niche: a comparative view.
J. A. Martinez-Agosto, H. K.A. Mikkola, V. Hartenstein, and U. Banerjee (2007)
Genes & Dev. 21, 3044-3060
   Abstract »    Full Text »    PDF »
Stem cells and their niche: an inseparable relationship.
T. Xie and L. Li (2007)
Development 134, 2001-2006
   Abstract »    Full Text »    PDF »
Drosophila mojoless, a Retroposed GSK-3, Has Functionally Diverged to Acquire an Essential Role in Male Fertility.
R. Kalamegham, D. Sturgill, E. Siegfried, and B. Oliver (2007)
Mol. Biol. Evol. 24, 732-742
   Abstract »    Full Text »    PDF »
Multipotent Drosophila Intestinal Stem Cells Specify Daughter Cell Fates by Differential Notch Signaling.
B. Ohlstein and A. Spradling (2007)
Science 315, 988-992
   Abstract »    Full Text »    PDF »
Asymmetric Inheritance of Mother Versus Daughter Centrosome in Stem Cell Division.
Y. M. Yamashita, A. P. Mahowald, J. R. Perlin, and M. T. Fuller (2007)
Science 315, 518-521
   Abstract »    Full Text »    PDF »
The control of sexual identity in the Drosophila germline.
A. Casper and M. Van Doren (2006)
Development 133, 2783-2791
   Abstract »    Full Text »    PDF »
JAK/STAT signalling in Drosophila: insights into conserved regulatory and cellular functions..
N. I. Arbouzova and M. P. Zeidler (2006)
Development 133, 2605-2616
   Abstract »    Full Text »    PDF »
Non-cell-autonomous action of STAT3 in maintenance of neural precursor cells in the mouse neocortex.
T. Yoshimatsu, D. Kawaguchi, K. Oishi, K. Takeda, S. Akira, N. Masuyama, and Y. Gotoh (2006)
Development 133, 2553-2563
   Abstract »    Full Text »    PDF »
Glial cell line-derived neurotrophic factor maintains a POZ-itive influence on stem cells.
C. Payne and R. E. Braun (2006)
PNAS 103, 9751-9752
   Full Text »    PDF »
The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity.
N. A. Siddall, E. A. McLaughlin, N. L. Marriner, and G. R. Hime (2006)
PNAS 103, 8402-8407
   Abstract »    Full Text »    PDF »
Hematopoietic-Osteoblastic Interactions in the Hematopoietic Stem Cell Niche.
L. M. Calvi (2006)
IBMS BoneKEy 3, 10-18
   Abstract »    Full Text »    PDF »
Identification of Drosophila Genes Modulating Janus Kinase/Signal Transducer and Activator of Transcription Signal Transduction.
T. Mukherjee, U. Schafer, and M. P. Zeidler (2006)
Genetics 172, 1683-1697
   Abstract »    Full Text »    PDF »
Molecular characterization of melanocyte stem cells in their niche.
M. Osawa, G. Egawa, S.-S. Mak, M. Moriyama, R. Freter, S. Yonetani, F. Beermann, and S.-I. Nishikawa (2005)
Development 132, 5589-5599
   Abstract »    Full Text »    PDF »
The Septin-Binding Protein Anillin Is Overexpressed in Diverse Human Tumors.
P. A. Hall, C. B. Todd, P. L. Hyland, S. S. McDade, H. Grabsch, M. Dattani, K. J. Hillan, and S.E. H. Russell (2005)
Clin. Cancer Res. 11, 6780-6786
   Abstract »    Full Text »    PDF »
Drosophila Myt1 is a Cdk1 inhibitory kinase that regulates multiple aspects of cell cycle behavior during gametogenesis.
Z. Jin, E. M. Homola, P. Goldbach, Y. Choi, J. A. Brill, and S. D. Campbell (2005)
Development 132, 4075-4085
   Abstract »    Full Text »    PDF »
Stimulation of Paracrine Pathways With Growth Factors Enhances Embryonic Stem Cell Engraftment and Host-Specific Differentiation in the Heart After Ischemic Myocardial Injury.
T. Kofidis, J. L. de Bruin, T. Yamane, M. Tanaka, D. R. Lebl, R.-J. Swijnenburg, I. L. Weissman, and R. C. Robbins (2005)
Circulation 111, 2486-2493
   Abstract »    Full Text »    PDF »
Long-Term Culture of Mouse Male Germline Stem Cells Under Serum-or Feeder-Free Conditions.
M. Kanatsu-Shinohara, H. Miki, K. Inoue, N. Ogonuki, S. Toyokuni, A. Ogura, and T. Shinohara (2005)
Biol Reprod 72, 985-991
   Abstract »    Full Text »    PDF »
Signaling in stem cell niches: lessons from the Drosophila germline.
Y. M. Yamashita, M. T. Fuller, and D. L. Jones (2005)
J. Cell Sci. 118, 665-672
   Abstract »    Full Text »    PDF »
How different is Venus from Mars? The genetics of germ-line stem cells in Drosophila females and males.
L. Gilboa and R. Lehmann (2004)
Development 131, 4895-4905
   Abstract »    Full Text »    PDF »
A Misexpression Screen Reveals Effects of bag-of-marbles and TGF{beta} Class Signaling on the Drosophila Male Germ-Line Stem Cell Lineage.
C. Schulz, A. A. Kiger, S. I. Tazuke, Y. M. Yamashita, L. C. Pantalena-Filho, D. L. Jones, C. G. Wood, and M. T. Fuller (2004)
Genetics 167, 707-723
   Abstract »    Full Text »    PDF »
Regeneration of Male Germline Stem Cells by Spermatogonial Dedifferentiation in Vivo.
C. Brawley and E. Matunis (2004)
Science 304, 1331-1334
   Abstract »    Full Text »    PDF »
Cytokines regulate fibroblast-like synovial cell differentiation to adipocyte-like cells.
S. Yamasaki, T. Nakashima, A. Kawakami, T. Miyashita, F. Tanaka, H. Ida, K. Migita, T. Origuchi, and K. Eguchi (2004)
Rheumatology 43, 448-452
   Abstract »    Full Text »    PDF »
Gbb/Bmp signaling is essential for maintaining germline stem cells and for repressing bam transcription in the Drosophila testis.
E. Kawase, M. D. Wong, B. C. Ding, and T. Xie (2004)
Development 131, 1365-1375
   Abstract »    Full Text »    PDF »
CD9 Is a Surface Marker on Mouse and Rat Male Germline Stem Cells.
M. Kanatsu-Shinohara, S. Toyokuni, and T. Shinohara (2004)
Biol Reprod 70, 70-75
   Abstract »    Full Text »    PDF »
A Sensitized Genetic Screen to Identify Novel Regulators and Components of the Drosophila Janus Kinase/Signal Transducer and Activator of Transcription Pathway.
E. A. Bach, S. Vincent, M. P. Zeidler, and N. Perrimon (2003)
Genetics 165, 1149-1166
   Abstract »    Full Text »    PDF »
A definitive role of Shp-2 tyrosine phosphatase in mediating embryonic stem cell differentiation and hematopoiesis.
R. J. Chan, S. A. Johnson, Y. Li, M. C. Yoder, and G.-S. Feng (2003)
Blood 102, 2074-2080
   Abstract »    Full Text »    PDF »
Orientation of Asymmetric Stem Cell Division by the APC Tumor Suppressor and Centrosome.
Y. M. Yamashita, D. L. Jones, and M. T. Fuller (2003)
Science 301, 1547-1550
   Abstract »    Full Text »    PDF »
Hematopoietic stem cells: can old cells learn new tricks?.
A. D. Ho and M. Punzel (2003)
J. Leukoc. Biol. 73, 547-555
   Abstract »    Full Text »    PDF »
A discrete transcriptional silencer in the bam gene determines asymmetric division of the Drosophila germline stem cell.
D. Chen and D. M. McKearin (2003)
Development 130, 1159-1170
   Abstract »    Full Text »    PDF »
A germline-specific gap junction protein required for survival of differentiating early germ cells.
S. I. Tazuke, C. Schulz, L. Gilboa, M. Fogarty, A. P. Mahowald, A. Guichet, A. Ephrussi, C. G. Wood, R. Lehmann, and M. T. Fuller (2003)
Development 129, 2529-2539
   Abstract »    Full Text »    PDF »
Restoration of Spermatogenesis in Infertile Mice by Sertoli Cell Transplantation.
T. Shinohara, K. E. Orwig, M. R. Avarbock, and R. L. Brinster (2003)
Biol Reprod 68, 1064-1071
   Abstract »    Full Text »    PDF »
SCARECROW is involved in positioning the stem cell niche in the Arabidopsis root meristem.
S. Sabatini, R. Heidstra, M. Wildwater, and B. Scheres (2003)
Genes & Dev. 17, 354-358
   Abstract »    Full Text »    PDF »
Localized JAK/STAT signaling is required for oriented cell rearrangement in a tubular epithelium.
K. A. Johansen, D. D. Iwaki, and J. A. Lengyel (2003)
Development 130, 135-145
   Abstract »    Full Text »    PDF »
Cell intrinsic defects in cytokine responsiveness of STAT5-deficient hematopoietic stem cells.
H. L. Bradley, T. S. Hawley, and K. D. Bunting (2002)
Blood 100, 3983-3989
   Abstract »    Full Text »    PDF »
From the Cover: DE-cadherin-mediated cell adhesion is essential for maintaining somatic stem cells in the Drosophila ovary.
X. Song and T. Xie (2002)
PNAS 99, 14813-14818
   Abstract »    Full Text »    PDF »
"Stemness": Transcriptional Profiling of Embryonic and Adult Stem Cells.
M. Ramalho-Santos, S. Yoon, Y. Matsuzaki, R. C. Mulligan, and D. A. Melton (2002)
Science 298, 597-600
   Abstract »    Full Text »    PDF »
Stimulation of 3T3-L1 Adipogenesis by Signal Transducer and Activator of Transcription 5.
R. Nanbu-Wakao, Y. Morikawa, I. Matsumura, Y. Masuho, M.-a. Muramatsu, E. Senba, and H. Wakao (2002)
Mol. Endocrinol. 16, 1565-1576
   Abstract »    Full Text »    PDF »
Stem Cell Self-Renewal Specified by JAK-STAT Activation in Response to a Support Cell Cue.
A. A. Kiger, D. L. Jones, C. Schulz, M. B. Rogers, and M. T. Fuller (2001)
Science 294, 2542-2545
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)