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Science 30 November 2001:
Vol. 294. no. 5548, pp. 1929 - 1932
DOI: 10.1126/science.1064103
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Reports

Real-Time Single-Molecule Imaging of the Infection Pathway of an Adeno-Associated Virus

Georg Seisenberger,1 Martin U. Ried,2 Thomas Endreß,1 Hildegard Büning,2 Michael Hallek,23 Christoph Bräuchle1*

We describe a method, based on single-molecule imaging, that allows the real-time visualization of the infection pathway of single viruses in living cells, each labeled with only one fluorescent dye molecule. The tracking of single viruses removes ensemble averaging. Diffusion trajectories with high spatial and time resolution show various modes of motion of adeno-associated viruses (AAV) during their infection pathway into living HeLa cells: (i) consecutive virus touching at the cell surface and fast endocytosis; (ii) free and anomalous diffusion of the endosome and the virus in the cytoplasm and the nucleus; and (iii) directed motion by motor proteins in the cytoplasm and in nuclear tubular structures. The real-time visualization of the infection pathway of single AAVs shows a much faster infection than was generally observed so far.

1 Department Chemie, Ludwig-Maximilians-Universität München, Butenandtstraße 11, D-81377 München, Germany.
2 Genzentrum, Laboratorium für Molekulare Biologie, Medizinische Klinik III, Klinikum Großhadern, Ludwig-Maximilians-Universität München, Feodor-Lynen-Straße 25, D-81377 München, Germany.
3 GSF-National Center for Research and Environment, KKG Gentherapie, Marchioninistraße 25, D-81377, Germany.
*   To whom correspondence should be addressed. E-mail: christoph.braeuchle{at}cup.uni-muenchen.de

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