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Science 16 November 2001:
Vol. 294. no. 5546, pp. 1531 - 1533
DOI: 10.1126/science.1063665
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Reports

Lack of Acrosome Formation in Hrb-Deficient Mice

Ningling Kang-Decker,1 George T. Mantchev,1 Subhash C. Juneja,1 Mark A. McNiven,23 Jan M. A. van Deursen1*

The sperm acrosome is essential for sperm-egg fusion and is often defective in men with nonobstructive infertility. Here we report that male mice with a null mutation in Hrb are infertile and display round-headed spermatozoa that lack an acrosome. In wild-type spermatids, Hrb is associated with the cytosolic surface of proacrosomic transport vesicles that fuse to create a single large acrosomic vesicle at step 3 of spermiogenesis. Although proacrosomic vesicles form in spermatids that lack Hrb, the vesicles are unable to fuse, blocking acrosome development at step 2. We conclude that Hrb is required for docking and/or fusion of proacrosomic vesicles during acrosome biogenesis.

1 Department of Pediatrics and Adolescent Medicine,
2 Department of Biochemistry and Molecular Biology,
3 Department of Gastroenterology and Hepatology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
*   To whom correspondence should be addressed. E-mail: vandeursen.jan{at}mayo.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)