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Originally published in Science Express on 23 August 2001
Science 12 October 2001:
Vol. 294. no. 5541, pp. 361 - 364
DOI: 10.1126/science.1063611
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Reports

Genetic Basis for Activity Differences Between Vancomycin and Glycolipid Derivatives of Vancomycin

Ulrike S. Eggert,1 Natividad Ruiz,2 Brian V. Falcone,1 Arthur A. Branstrom,3 Robert C. Goldman,3 Thomas J. Silhavy,2 Daniel Kahne1*

Small molecules that affect specific protein functions can be valuable tools for dissecting complex cellular processes. Peptidoglycan synthesis and degradation is a process in bacteria that involves multiple enzymes under strict temporal and spatial regulation. We used a set of small molecules that inhibit the transglycosylation step of peptidoglycan synthesis to discover genes that help to regulate this process. We identified a gene responsible for the susceptibility of Escherichia coli cells to killing by glycolipid derivatives of vancomycin, thus establishing a genetic basis for activity differences between these compounds and vancomycin.

1 Department of Chemistry,
2 Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
3 Advanced Medicine Inc., 901 Gateway Boulevard, South San Francisco, CA 94080, USA.
*   To whom correspondence should be addressed. E-mail: dkahne{at}princeton.edu

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