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Science 21 September 2001:
Vol. 293. no. 5538, pp. 2266 - 2269
DOI: 10.1126/science.1063566
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Reports

Identification of Critical Staphylococcal Genes Using Conditional Phenotypes Generated by Antisense RNA

Yinduo Ji,1* Barbara Zhang,1dagger Stephanie F. Van , Horn,2 Patrick Warren,2 Gary Woodnutt,1 Martin K. R. Burnham,1 Martin Rosenberg1

Comprehensive genomic analysis of the important human pathogen Staphylococcus aureus was achieved by a strategy involving antisense technology in a regulatable gene expression system. In addition to known essential genes, many genes of unknown or poorly defined biological function were identified. This methodology allowed gene function to be characterized in a comprehensive, defined set of conditionally growth-defective/lethal isogenic strains. Quantitative titration of the conditional growth effect was performed either in bacterial culture or in an animal model of infection. This genomic strategy offers an approach to the identification of staphylococcal gene products that could serve as targets for antibiotic discovery.

1 Department of Microbiology,
2 Genetics Research, GlaxoSmithKline Pharmaceuticals Research and Development, Collegeville, PA 19426, USA.
*   To whom correspondence should be addressed at 1250 South Collegeville Road, UP1345, Collegeville, PA 19426, USA. E-mail: yinduo_ji-1{at}gsk.com

dagger    Present address: Pharmacia Corporation, Peapack, NJ 07977, USA.

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