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Science 7 September 2001:
Vol. 293. no. 5536, pp. 1836 - 1839
DOI: 10.1126/science.1062786
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Reports

Communication of the Position of Exon-Exon Junctions to the mRNA Surveillance Machinery by the Protein RNPS1

Jens Lykke-Andersen,* Mei-Di Shu, Joan A. Steitzdagger

In mammalian cells, splice junctions play a dual role in mRNA quality control: They mediate selective nuclear export of mature mRNA and they serve as a mark for mRNA surveillance, which subjects aberrant mRNAs with premature termination codons to nonsense-mediated decay (NMD). Here, we demonstrate that the protein RNPS1, a component of the postsplicing complex that is deposited 5' to exon-exon junctions, interacts with the evolutionarily conserved human Upf complex, a central component of NMD. Significantly, RNPS1 triggers NMD when tethered to the 3' untranslated region of beta -globin mRNA, demonstrating its role as a subunit of the postsplicing complex directly involved in mRNA surveillance.

Howard Hughes Medical Institute, Molecular Biochemistry and Biophysics, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.
*   Present address: Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA.

dagger    To whom correspondence should be addressed: E-mail: joan.steitz{at}yale.edu.

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