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Originally published in Science Express on 26 July 2001
Science 7 September 2001:
Vol. 293. no. 5536, pp. 1820 - 1824
DOI: 10.1126/science.1060580
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Reports

Segregation of Human Neural Stem Cells in the Developing Primate Forebrain

Václav Ourednik,1*dagger Jitka Ourednik,1* Jonathan D. Flax,1 W. Michael Zawada,2 Cynthia Hutt,2 Chunhua Yang,1 Kook I. Park,13 Seung U. Kim,4 Richard L. Sidman,5 Curt R. Freed,2ddagger Evan Y. Snyder1dagger ddagger

Many central nervous system regions at all stages of life contain neural stem cells (NSCs). We explored how these disparate NSC pools might emerge. A traceable clone of human NSCs was implanted intraventricularly to allow its integration into cerebral germinal zones of Old World monkey fetuses. The NSCs distributed into two subpopulations: One contributed to corticogenesis by migrating along radial glia to temporally appropriate layers of the cortical plate and differentiating into lamina-appropriate neurons or glia; the other remained undifferentiated and contributed to a secondary germinal zone (the subventricular zone) with occasional members interspersed throughout brain parenchyma. An early neurogenetic program allocates the progeny of NSCs either immediately for organogenesis or to undifferentiated pools for later use in the "postdevelopmental" brain.

1 Departments of Pediatrics, Neurosurgery, and Neurology, Children's Hospital, Harvard Medical School, 248 Enders Building, 300 Longwood Avenue, Boston, MA 02115, USA.
2 Department of Medicine and Pharmacology and the Neuroscience Program, University of Colorado School of Medicine, 4200 East 9th Avenue, Denver, CO 80220, USA.
3 Department of Pediatrics, College of Medicine, Yonsei University, Seoul, Korea.
4 Department of Neurology, University of British Columbia, Koerner Pavilion, 211 Wesbrook Mall, Vancouver, BC, Canada V6T 2B5.
5 Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, LMRC, 221 Longwood Avenue, Boston, MA 02115, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed at present address: Beth Israel Deaconess Medical Center, Department of Neurology, 855 Harvard Institute of Medicine, 77 Avenue Louis Pasteur, Boston, MA 02115, USA. E-mail: esnyder1{at}caregroup.harvard.edu or vouredni{at}caregroup.harvard.edu

ddagger    Co-senior authors.

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