Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 17 August 2001:
Vol. 293. no. 5533, pp. 1289 - 1292
DOI: 10.1126/science.1062711
Prev | Table of Contents | Next

Reports

Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species

Yi Cui,1* Qingqiao Wei,1* Hongkun Park,1 Charles M. Lieber12dagger

Boron-doped silicon nanowires (SiNWs) were used to create highly sensitive, real-time electrically based sensors for biological and chemical species. Amine- and oxide-functionalized SiNWs exhibit pH-dependent conductance that was linear over a large dynamic range and could be understood in terms of the change in surface charge during protonation and deprotonation. Biotin-modified SiNWs were used to detect streptavidin down to at least a picomolar concentration range. In addition, antigen-functionalized SiNWs show reversible antibody binding and concentration-dependent detection in real time. Lastly, detection of the reversible binding of the metabolic indicator Ca2+ was demonstrated. The small size and capability of these semiconductor nanowires for sensitive, label-free, real-time detection of a wide range of chemical and biological species could be exploited in array-based screening and in vivo diagnostics.

1 Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
2 Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138 USA.
*   These authors contributed equally to the work.

dagger    To whom correspondence should be addressed. E-mail: cml{at}cmliris.harvard.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
From the Cover: Bioelectronic silicon nanowire devices using functional membrane proteins.
N. Misra, J. A. Martinez, S.-C. J. Huang, Y. Wang, P. Stroeve, C. P. Grigoropoulos, and A. Noy (2009)
PNAS 106, 13780-13784
   Abstract »    Full Text »    PDF »
Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays.
A. Bajaj, O. R. Miranda, I.-B. Kim, R. L. Phillips, D. J. Jerry, U. H. F. Bunz, and V. M. Rotello (2009)
PNAS 106, 10912-10916
   Abstract »    Full Text »    PDF »
Nanorobot for Brain Aneurysm.
A. Cavalcanti, B. Shirinzadeh, T. Fukuda, and S. Ikeda (2009)
The International Journal of Robotics Research 28, 558-570
   Abstract »    PDF »
Comment on "Detection, Stimulation, and Inhibition of Neuronal Signals with High-Density Nanowire Transistor Arrays".
P. Fromherz and M. Voelker (2009)
Science 323, 1429b
   Abstract »    Full Text »    PDF »
Response to Comment on "Detection, Stimulation, and Inhibition of Neuronal Signals with High-Density Nanowire Transistor Arrays".
B. P. Timko, F. Patolsky, and C. M. Lieber (2009)
Science 323, 1429c
   Abstract »    Full Text »    PDF »
4D Electron Diffraction Reveals Correlated Unidirectional Behavior in Zinc Oxide Nanowires.
D.-S. Yang, C. Lao, and A. H. Zewail (2008)
Science 321, 1660-1664
   Abstract »    Full Text »    PDF »
Water-stable organic transistors and their application in chemical and biological sensors.
M. E. Roberts, S. C. B. Mannsfeld, N. Queralto, C. Reese, J. Locklin, W. Knoll, and Z. Bao (2008)
PNAS 105, 12134-12139
   Abstract »    Full Text »    PDF »
Large-scale, heterogeneous integration of nanowire arrays for image sensor circuitry.
Z. Fan, J. C. Ho, Z. A. Jacobson, H. Razavi, and A. Javey (2008)
PNAS 105, 11066-11070
   Abstract »    Full Text »    PDF »
Detection, Stimulation, and Inhibition of Neuronal Signals with High-Density Nanowire Transistor Arrays.
F. Patolsky, B. P. Timko, G. Yu, Y. Fang, A. B. Greytak, G. Zheng, and C. M. Lieber (2006)
Science 313, 1100-1104
   Abstract »    Full Text »    PDF »
Nanotechnology for the biologist.
S. E. McNeil (2005)
J. Leukoc. Biol. 78, 585-594
   Abstract »    Full Text »    PDF »
Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destruction.
N. W. Shi Kam, M. O'Connell, J. A. Wisdom, and H. Dai (2005)
PNAS 102, 11600-11605
   Abstract »    Full Text »    PDF »
Label-free detection of small-molecule-protein interactions by using nanowire nanosensors.
W. U. Wang, C. Chen, K.-h. Lin, Y. Fang, and C. M. Lieber (2005)
PNAS 102, 3208-3212
   Abstract »    Full Text »    PDF »
Systems Biology and New Technologies Enable Predictive and Preventative Medicine.
L. Hood, J. R. Heath, M. E. Phelps, and B. Lin (2004)
Science 306, 640-643
   Abstract »    Full Text »    PDF »
Miniaturized Multiplex Label-Free Electronic Chip for Rapid Nucleic Acid Analysis Based on Carbon Nanotube Nanoelectrode Arrays.
J. E. Koehne, H. Chen, A. M. Cassell, Q. Ye, J. Han, M. Meyyappan, and J. Li (2004)
Clin. Chem. 50, 1886-1893
   Abstract »    Full Text »    PDF »
Electrical detection of single viruses.
F. Patolsky, G. Zheng, O. Hayden, M. Lakadamyali, X. Zhuang, and C. M. Lieber (2004)
PNAS 101, 14017-14022
   Abstract »    Full Text »    PDF »
Recommendations of the National Heart, Lung, and Blood Institute Nanotechnology Working Group.
D. B. Buxton, S. C. Lee, S. A. Wickline, M. Ferrari, and for the Working Group Members (2003)
Circulation 108, 2737-2742
   Abstract »    Full Text »    PDF »
Nanowire Crossbar Arrays as Address Decoders for Integrated Nanosystems.
Z. Zhong, D. Wang, Y. Cui, M. W. Bockrath, and C. M. Lieber (2003)
Science 302, 1377-1379
   Abstract »    Full Text »    PDF »
Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors.
R. J. Chen, S. Bangsaruntip, K. A. Drouvalakis, N. Wong Shi Kam, M. Shim, Y. Li, W. Kim, P. J. Utz, and H. Dai (2003)
PNAS 100, 4984-4989
   Abstract »    Full Text »    PDF »
Electronic detection of DNA by its intrinsic molecular charge.
J. Fritz, E. B. Cooper, S. Gaudet, P. K. Sorger, and S. R. Manalis (2002)
PNAS 99, 14142-14146
   Abstract »    Full Text »    PDF »
Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA Detection.
Y. C. Cao, R. Jin, and C. A. Mirkin (2002)
Science 297, 1536-1540
   Abstract »    Full Text »    PDF »
Direct Patterning of Modified Oligonucleotides on Metals and Insulators by Dip-Pen Nanolithography.
L. M. Demers, D. S. Ginger, S.-J. Park, Z. Li, S.-W. Chung, and C. A. Mirkin (2002)
Science 296, 1836-1838
   Abstract »    Full Text »    PDF »
Photoinduced Conversion of Silver Nanospheres to Nanoprisms.
R. Jin, Y. Cao, C. A. Mirkin, K. L. Kelly, G. C. Schatz, and J. G. Zheng (2001)
Science 294, 1901-1903
   Abstract »    Full Text »    PDF »
Dielectrophoretic Assembly of Electrically Functional Microwires from Nanoparticle Suspensions.
K. D. Hermanson, S. O. Lumsdon, J. P. Williams, E. W. Kaler, and O. D. Velev (2001)
Science 294, 1082-1086
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)