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Science 6 July 2001:
Vol. 293. no. 5527, pp. 98 - 101
DOI: 10.1126/science.293.5527.98
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Reports

A beta 2 Adrenergic Receptor Signaling Complex Assembled with the Ca2+ Channel Cav1.2

Monika A. Davare,1* Vladimir Avdonin,2 Duane D. Hall,1dagger Erik M. Peden,1 Alain Burette,3 Richard J. Weinberg,3 Mary C. Horne,1dagger Toshinori Hoshi,2 Johannes W. Hell1*dagger ddagger

The existence of a large number of receptors coupled to heterotrimeric guanine nucleotide binding proteins (G proteins) raises the question of how a particular receptor selectively regulates specific targets. We provide insight into this question by identifying a prototypical macromolecular signaling complex. The beta 2 adrenergic receptor was found to be directly associated with one of its ultimate effectors, the class C L-type calcium channel Cav1.2. This complex also contained a G protein, an adenylyl cyclase, cyclic adenosine monophosphate-dependent protein kinase, and the counterbalancing phosphatase PP2A. Our electrophysiological recordings from hippocampal neurons demonstrate highly localized signal transduction from the receptor to the channel. The assembly of this signaling complex provides a mechanism that ensures specific and rapid signaling by a G protein-coupled receptor.

1 Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA.
2 Department of Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USA.
3 Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill, NC 27599, USA.
*   Present address: Vollum Institute, Oregon Health Sciences University, Portland, OR 97201, USA.

dagger    Present address: Department of Pharmacology, University of Iowa, Iowa, City, IA 52242, USA.

ddagger    To whom correspondence should be addressed. E-mail: johannes-hell{at}uiowa.edu

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   Abstract »    Full Text »    PDF »
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J. H. Zippin, J. Farrell, D. Huron, M. Kamenetsky, K. C. Hess, D. A. Fischman, L. R. Levin, and J. Buck (2004)
J. Cell Biol. 164, 527-534
   Abstract »    Full Text »    PDF »
Role of Lipid Microdomains in P/Q-type Calcium Channel (Cav2.1) Clustering and Function in Presynaptic Membranes.
E. Taverna, E. Saba, J. Rowe, M. Francolini, F. Clementi, and P. Rosa (2004)
J. Biol. Chem. 279, 5127-5134
   Abstract »    Full Text »    PDF »
G Protein Signaling: Insights from New Structures.
A. M. Preininger and H. E. Hamm (2004)
Sci. STKE 2004, re3
   Abstract »    Full Text »    PDF »
Increased phosphorylation of the neuronal L-type Ca2+ channel Cav1.2 during aging.
M. A. Davare and J. W. Hell (2003)
PNAS 100, 16018-16023
   Abstract »    Full Text »    PDF »
Diminished Surface Clustering and Increased Perinuclear Accumulation of Large Conductance Ca2+-activated K+ Channel in Mouse Myometrium with Pregnancy.
M. Eghbali, L. Toro, and E. Stefani (2003)
J. Biol. Chem. 278, 45311-45317
   Abstract »    Full Text »    PDF »
What Is the Role of {beta}-Adrenergic Signaling in Heart Failure?.
M. J. Lohse, S. Engelhardt, and T. Eschenhagen (2003)
Circ. Res. 93, 896-906
   Abstract »    Full Text »    PDF »
Phosphoinositide 3-Kinase Cascade Facilitates {micro}-Opioid Desensitization in Sensory Neurons by Altering G-Protein-Effector Interactions.
M. Tan, M. Groszer, A. M. Tan, A. Pandya, X. Liu, and C.-W. Xie (2003)
J. Neurosci. 23, 10292-10301
   Abstract »    Full Text »    PDF »
The third intracellular loop and carboxyl tail of neurokinin 1 and 3 receptors determine interactions with {beta}-arrestins.
F. Schmidlin, D. Roosterman, and N. W. Bunnett (2003)
Am J Physiol Cell Physiol 285, C945-C958
   Abstract »    Full Text »    PDF »
The PDZ-binding motif of the {beta}2-adrenoceptor is essential for physiologic signaling and trafficking in cardiac myocytes.
Y. Xiang and B. Kobilka (2003)
PNAS 100, 10776-10781
   Abstract »    Full Text »    PDF »
Protein Kinase A and G Protein-coupled Receptor Kinase Phosphorylation Mediates {beta}-1 Adrenergic Receptor Endocytosis through Different Pathways.
A. Rapacciuolo, S. Suvarna, L. Barki-Harrington, L. M. Luttrell, M. Cong, R. J. Lefkowitz, and H. A. Rockman (2003)
J. Biol. Chem. 278, 35403-35411
   Abstract »    Full Text »    PDF »
Antagonistic Modulation of a Hyperpolarization-Activated Cl- Current in Aplysia Sensory Neurons by SCPB and FMRFamide.
N. Buttner and S. A. Siegelbaum (2003)
J Neurophysiol 90, 586-598
   Abstract »    Full Text »    PDF »
Sodium/Calcium Exchanger (NCX1) Macromolecular Complex.
D. H. Schulze, M. Muqhal, W. J. Lederer, and A. M. Ruknudin (2003)
J. Biol. Chem. 278, 28849-28855
   Abstract »    Full Text »    PDF »
NMDA and {beta}1-Adrenergic Receptors Differentially Signal Phosphorylation of Glutamate Receptor Type 1 in Area CA1 of Hippocampus.
A. M. Vanhoose and D. G. Winder (2003)
J. Neurosci. 23, 5827-5834
   Abstract »    Full Text »    PDF »
Direct Interaction and Functional Coupling between Metabotropic Glutamate Receptor Subtype 1 and Voltage-sensitive Cav2.1 Ca2+ Channel.
J. Kitano, M. Nishida, Y. Itsukaichi, I. Minami, M. Ogawa, T. Hirano, Y. Mori, and S. Nakanishi (2003)
J. Biol. Chem. 278, 25101-25108
   Abstract »    Full Text »    PDF »
PP2A Activation by {beta}2-Adrenergic Receptor Agonists: NOVEL REGULATORY MECHANISM OF KERATINOCYTE MIGRATION.
C. E. Pullar, J. Chen, and R. R. Isseroff (2003)
J. Biol. Chem. 278, 22555-22562
   Abstract »    Full Text »    PDF »
T-Tubule Function in Mammalian Cardiac Myocytes.
F. Brette and C. Orchard (2003)
Circ. Res. 92, 1182-1192
   Abstract »    Full Text »    PDF »
RGS3 mediates a calcium-dependent termination of G protein signaling in sensory neurons.
P. Tosetti, N. Pathak, M. H. Jacob, and K. Dunlap (2003)
PNAS 100, 7337-7342
   Abstract »    Full Text »    PDF »
Dynamic Imaging in Living Cells: Windows into Local Signaling.
D. M. Bers (2003)
Sci. STKE 2003, pe13
   Abstract »    Full Text »    PDF »
Identification and Functional Analysis of Two Ca2+-binding EF-hand Motifs in the B"/PR72 Subunit of Protein Phosphatase 2A.
V. Janssens, J. Jordens, I. Stevens, C. Van Hoof, E. Martens, H. De Smedt, Y. Engelborghs, E. Waelkens, and J. Goris (2003)
J. Biol. Chem. 278, 10697-10706
   Abstract »    Full Text »    PDF »
Pituitary Adenylate Cyclase-activating Polypeptide (PACAP(1-38)) Enhances N-Methyl-D-aspartate Receptor Function and Brain-derived Neurotrophic Factor Expression via RACK1.
R. Yaka, D.-Y. He, K. Phamluong, and D. Ron (2003)
J. Biol. Chem. 278, 9630-9638
   Abstract »    Full Text »    PDF »


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