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Science 1 June 2001:
Vol. 292. no. 5522, pp. 1716 - 1718
DOI: 10.1126/science.1060896
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Reports

Binding of GGA2 to the Lysosomal Enzyme Sorting Motif of the Mannose 6-Phosphate Receptor

Yunxiang Zhu,1 Balraj Doray,1 Anssi Poussu,2 Veli-Pekka Lehto,2 Stuart Kornfeld1*

The GGAs are a multidomain protein family implicated in protein trafficking between the Golgi and endosomes. Here, the VHS domain of GGA2 was shown to bind to the acidic cluster-dileucine motif in the cytoplasmic tail of the cation-independent mannose 6-phosphate receptor (CI-MPR). Receptors with mutations in this motif were defective in lysosomal enzyme sorting. The hinge domain of GGA2 bound clathrin, suggesting that GGA2 could be a link between cargo molecules and clathrin-coated vesicle assembly. Thus, GGA2 binding to the CI-MPR is important for lysosomal enzyme targeting.

1 Department of Internal Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
2 Department of Pathology, University of Oulu, FIN-90410 Oulu, Finland.
*   To whom correspondence should be addressed. E-mail: skornfel{at}im.wustl.edu

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