Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 4 May 2001:
Vol. 292. no. 5518, pp. 897 - 902
DOI: 10.1126/science.1060612
Prev | Table of Contents | Next

Research Articles

Recognition of Cognate Transfer RNA by the 30S Ribosomal Subunit

James M. Ogle, Ditlev E. Brodersen, William M. Clemons Jr., Michael J. Tarry, Andrew P. Carter, V. Ramakrishnan*

Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces global domain movements of the 30S subunit and changes in the conformation of the universally conserved and essential bases A1492, A1493, and G530 of 16S RNA. These bases interact intimately with the minor groove of the first two base pairs between the codon and anticodon, thus sensing Watson-Crick base-pairing geometry and discriminating against near-cognate tRNA. The third, or "wobble," position of the codon is free to accommodate certain noncanonical base pairs. By partially inducing these structural changes, paromomycin facilitates binding of near-cognate tRNAs.

MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
*   To whom correspondence should be addressed. E-mail: ramak{at}mrc-lmb.cam.ac.uk

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
RNA Helicase Prp43 and Its Co-factor Pfa1 Promote 20 to 18 S rRNA Processing Catalyzed by the Endonuclease Nob1.
B. Pertschy, C. Schneider, M. Gnadig, T. Schafer, D. Tollervey, and E. Hurt (2009)
J. Biol. Chem. 284, 35079-35091
   Abstract »    Full Text »    PDF »
The Ribosome: Some Hard Facts about Its Structure and Hot Air about Its Evolution.
V. Ramakrishnan (2009)
Cold Spring Harb Symp Quant Biol
   Abstract »    PDF »
Free energy calculation of modified base-pair formation in explicit solvent: A predictive model.
F. A.P. Vendeix, A. M. Munoz, and P. F. Agris (2009)
RNA 15, 2278-2287
   Abstract »    Full Text »    PDF »
Ribosomal Protein S12 and Aminoglycoside Antibiotics Modulate A-site mRNA Cleavage and Transfer-Messenger RNA Activity in Escherichia coli.
L. E. Holberger and C. S. Hayes (2009)
J. Biol. Chem. 284, 32188-32200
   Abstract »    Full Text »    PDF »
The Crystal Structure of the Ribosome Bound to EF-Tu and Aminoacyl-tRNA.
T. M. Schmeing, R. M. Voorhees, A. C. Kelley, Y.-G. Gao, F. V. Murphy IV, J. R. Weir, and V. Ramakrishnan (2009)
Science 326, 688-694
   Abstract »    Full Text »    PDF »
The Structure of the Ribosome with Elongation Factor G Trapped in the Posttranslocational State.
Y.-G. Gao, M. Selmer, C. M. Dunham, A. Weixlbaumer, A. C. Kelley, and V. Ramakrishnan (2009)
Science 326, 694-699
   Abstract »    Full Text »    PDF »
Loss of rRNA modifications in the decoding center of the ribosome impairs translation and strongly delays pre-rRNA processing.
X.-h. Liang, Q. Liu, and M. J. Fournier (2009)
RNA 15, 1716-1728
   Abstract »    Full Text »    PDF »
The Ribosome as a Conveying Thermal Ratchet Machine.
A. S. Spirin (2009)
J. Biol. Chem. 284, 21103-21119
   Full Text »    PDF »
Fluorescently labeled ribosomes as a tool for analyzing antibiotic binding.
B. Llano-Sotelo, R. P. Hickerson, L. Lancaster, H. F. Noller, and A. S. Mankin (2009)
RNA 15, 1597-1604
   Abstract »    Full Text »    PDF »
Accuracy modulating mutations of the ribosomal protein S4-S5 interface do not necessarily destabilize the rps4-rps5 protein-protein interaction.
H. Vallabhaneni and P. J. Farabaugh (2009)
RNA 15, 1100-1109
   Abstract »    Full Text »    PDF »
A Gripping Tale of Ribosomal Frameshifting: Extragenic Suppressors of Frameshift Mutations Spotlight P-Site Realignment.
J. F. Atkins and G. R. Bjork (2009)
Microbiol. Mol. Biol. Rev. 73, 178-210
   Abstract »    Full Text »    PDF »
Specificity of the ribosomal A site for aminoacyl-tRNAs.
T. Dale, R. P. Fahlman, M. Olejniczak, and O. C. Uhlenbeck (2009)
Nucleic Acids Res. 37, 1202-1210
   Abstract »    Full Text »    PDF »
A signal relay between ribosomal protein S12 and elongation factor EF-Tu during decoding of mRNA.
S. T. Gregory, J. F. Carr, and A. E. Dahlberg (2009)
RNA 15, 208-214
   Abstract »    Full Text »    PDF »
Crystal structure of a translation termination complex formed with release factor RF2.
A. Korostelev, H. Asahara, L. Lancaster, M. Laurberg, A. Hirschi, J. Zhu, S. Trakhanov, W. G. Scott, and H. F. Noller (2008)
PNAS 105, 19684-19689
   Abstract »    Full Text »    PDF »
Insights into Translational Termination from the Structure of RF2 Bound to the Ribosome.
A. Weixlbaumer, H. Jin, C. Neubauer, R. M. Voorhees, S. Petry, A. C. Kelley, and V. Ramakrishnan (2008)
Science 322, 953-956
   Abstract »    Full Text »    PDF »
Peptidyl-CCA deacylation on the ribosome promoted by induced fit and the O3'-hydroxyl group of A76 of the unacylated A-site tRNA.
M. Simonovic and T. A. Steitz (2008)
RNA 14, 2372-2378
   Abstract »    Full Text »    PDF »
Irreversible chemical steps control intersubunit dynamics during translation.
R. A. Marshall, M. Dorywalska, and J. D. Puglisi (2008)
PNAS 105, 15364-15369
   Abstract »    Full Text »    PDF »
Ribosomes bind leaderless mRNA in Escherichia coli through recognition of their 5'-terminal AUG.
J. E. Brock, S. Pourshahian, J. Giliberti, P. A. Limbach, and G. R. Janssen (2008)
RNA 14, 2159-2169
   Abstract »    Full Text »    PDF »
The sarcin-ricin loop of 23S rRNA is essential for assembly of the functional core of the 50S ribosomal subunit.
L. Lancaster, N. J. Lambert, E. J. Maklan, L. H. Horan, and H. F. Noller (2008)
RNA 14, 1999-2012
   Abstract »    Full Text »    PDF »
Crystal Structure of the Thermus thermophilus 16 S rRNA Methyltransferase RsmC in Complex with Cofactor and Substrate Guanosine.
H. Demirci, S. T. Gregory, A. E. Dahlberg, and G. Jogl (2008)
J. Biol. Chem. 283, 26548-26556
   Abstract »    Full Text »    PDF »
Critical Residues for Cofactor Binding and Catalytic Activity in the Aminoglycoside Resistance Methyltransferase Sgm.
M. Savic, T. Ilic-Tomic, R. Macmaster, B. Vasiljevic, and G. L. Conn (2008)
J. Bacteriol. 190, 5855-5861
   Abstract »    Full Text »    PDF »
Structural basis for hygromycin B inhibition of protein biosynthesis.
M. A. Borovinskaya, S. Shoji, K. Fredrick, and J. H.D. Cate (2008)
RNA 14, 1590-1599
   Abstract »    Full Text »    PDF »
Modified Uridines with C5-methylene Substituents at the First Position of the tRNA Anticodon Stabilize U{middle dot}G Wobble Pairing during Decoding.
S. Kurata, A. Weixlbaumer, T. Ohtsuki, T. Shimazaki, T. Wada, Y. Kirino, K. Takai, K. Watanabe, V. Ramakrishnan, and T. Suzuki (2008)
J. Biol. Chem. 283, 18801-18811
   Abstract »    Full Text »    PDF »
Degeneracy of the genetic code and stability of the base pair at the second position of the anticodon.
J. Lehmann and A. Libchaber (2008)
RNA 14, 1264-1269
   Abstract »    Full Text »    PDF »
The bacterial and mitochondrial ribosomal A-site molecular switches possess different conformational substates.
J. Kondo and E. Westhof (2008)
Nucleic Acids Res. 36, 2654-2666
   Abstract »    Full Text »    PDF »
Comprehensive features of natural and in vitro selected GNRA tetraloop-binding receptors.
C. Geary, S. Baudrey, and L. Jaeger (2008)
Nucleic Acids Res. 36, 1138-1152
   Abstract »    Full Text »    PDF »
Mitochondrial deafness alleles confer misreading of the genetic code.
S. N. Hobbie, C. M. Bruell, S. Akshay, S. K. Kalapala, D. Shcherbakov, and E. C. Bottger (2008)
PNAS 105, 3244-3249
   Abstract »    Full Text »    PDF »
An intact unfolded protein response in Trpt1 knockout mice reveals phylogenic divergence in pathways for RNA ligation.
H. P. Harding, J. G. Lackey, H.-C. Hsu, Y. Zhang, J. Deng, R.-M. Xu, M. J. Damha, and D. Ron (2008)
RNA 14, 225-232
   Abstract »    Full Text »    PDF »
Cross-crystal averaging reveals that the structure of the peptidyl-transferase center is the same in the 70S ribosome and the 50S subunit.
M. Simonovic and T. A. Steitz (2008)
PNAS 105, 500-505
   Abstract »    Full Text »    PDF »
Eukaryotic ribosomal RNA determinants of aminoglycoside resistance and their role in translational fidelity.
H. Fan-Minogue and D. M. Bedwell (2008)
RNA 14, 148-157
   Abstract »    Full Text »    PDF »
Inaugural Article: The process of mRNA-tRNA translocation.
J. Frank, H. Gao, J. Sengupta, N. Gao, and D. J. Taylor (2007)
PNAS 104, 19671-19678
   Abstract »    Full Text »    PDF »
Human ribosomal protein L13a is dispensable for canonical ribosome function but indispensable for efficient rRNA methylation.
S. Chaudhuri, K. Vyas, P. Kapasi, A. A. Komar, J. D. Dinman, S. Barik, and B. Mazumder (2007)
RNA 13, 2224-2237
   Abstract »    Full Text »    PDF »
The wobble hypothesis revisited: Uridine-5-oxyacetic acid is critical for reading of G-ending codons.
S. J. Nasvall, P. Chen, and G. R. Bjork (2007)
RNA 13, 2151-2164
   Abstract »    Full Text »    PDF »
Self-assembled lamellar complexes of siRNA with lipidic aminoglycoside derivatives promote efficient siRNA delivery and interference.
L. Desigaux, M. Sainlos, O. Lambert, R. Chevre, E. Letrou-Bonneval, J.-P. Vigneron, P. Lehn, J.-M. Lehn, and B. Pitard (2007)
PNAS 104, 16534-16539
   Abstract »    Full Text »    PDF »
Engineering the rRNA decoding site of eukaryotic cytosolic ribosomes in bacteria.
S. N. Hobbie, S. K. Kalapala, S. Akshay, C. Bruell, S. Schmidt, S. Dabow, A. Vasella, P. Sander, and E. C. Bottger (2007)
Nucleic Acids Res. 35, 6086-6093
   Abstract »    Full Text »    PDF »
From the Cover: The role of fluctuations in tRNA selection by the ribosome.
T.-H. Lee, S. C. Blanchard, H. D. Kim, J. D. Puglisi, and S. Chu (2007)
PNAS 104, 13661-13665
   Abstract »    Full Text »    PDF »
Testing constraints on rRNA bases that make nonsequence-specific contacts with the codon*anticodon complex in the ribosomal A site.
D. L. Taliaferro and P. J. Farabaugh (2007)
RNA 13, 1279-1286
   Abstract »    Full Text »    PDF »
Structures of tRNAs with an expanded anticodon loop in the decoding center of the 30S ribosomal subunit.
C. M. Dunham, M. Selmer, S. S. Phelps, A. C. Kelley, T. Suzuki, S. Joseph, and V. Ramakrishnan (2007)
RNA 13, 817-823
   Abstract »    Full Text »    PDF »
Anticodon-dependent conservation of bacterial tRNA gene sequences.
M. E. Saks and J. S. Conery (2007)
RNA 13, 651-660
   Abstract »    Full Text »    PDF »
Defining the Molecular Forces That Determine the Impact of Neomycin on Bacterial Protein Synthesis: Importance of the 2'-Amino Functionality.
C. M. Barbieri, M. Kaul, M. Bozza-Hingos, F. Zhao, Y. Tor, T. Hermann, and D. S. Pilch (2007)
Antimicrob. Agents Chemother. 51, 1760-1769
   Abstract »    Full Text »    PDF »
G-ribo: A new structural motif in ribosomal RNA.
S. V. Steinberg and Y. I. Boutorine (2007)
RNA 13, 549-554
   Abstract »    Full Text »    PDF »
Ribosomal RNA guanine-(N2)-methyltransferases and their targets.
P. V. Sergiev, A. A. Bogdanov, and O. A. Dontsova (2007)
Nucleic Acids Res. 35, 2295-2301
   Abstract »    Full Text »    PDF »
Intersubunit movement is required for ribosomal translocation.
L. H. Horan and H. F. Noller (2007)
PNAS 104, 4881-4885
   Abstract »    Full Text »    PDF »
Binding Mode of the First Aminoacyl-tRNA in Translation Initiation Mediated by Plautia stali Intestine Virus Internal Ribosome Entry Site.
H. Yamamoto, N. Nakashima, Y. Ikeda, and T. Uchiumi (2007)
J. Biol. Chem. 282, 7770-7776
   Abstract »    Full Text »    PDF »
New bioinformatic tools for analysis of nucleotide modifications in eukaryotic rRNA.
D. Piekna-Przybylska, W. A. Decatur, and M. J. Fournier (2007)
RNA 13, 305-312
   Abstract »    Full Text »    PDF »
L11 domain rearrangement upon binding to RNA and thiostrepton studied by NMR spectroscopy.
H. R. A. Jonker, S. Ilin, S. K. Grimm, J. Wohnert, and H. Schwalbe (2007)
Nucleic Acids Res. 35, 441-454
   Abstract »    Full Text »    PDF »
The frequency of translational misreading errors in E. coli is largely determined by tRNA competition.
E. B. Kramer and P. J. Farabaugh (2007)
RNA 13, 87-96
   Abstract »    Full Text »    PDF »
Inhibition of Antiassociation Activity of Translation Initiation Factor 3 by Paromomycin.
G. Hirokawa, H. Kaji, and A. Kaji (2007)
Antimicrob. Agents Chemother. 51, 175-180
   Abstract »    Full Text »    PDF »
Involvement of Helix 34 of 16 S rRNA in Decoding and Translocation on the Ribosome.
A. Kubarenko, P. Sergiev, W. Wintermeyer, O. Dontsova, and M. V. Rodnina (2006)
J. Biol. Chem. 281, 35235-35244
   Abstract »    Full Text »    PDF »
Molecular dynamics simulations of human Formula: the role of modified bases in mRNA recognition.
N. E. McCrate, M. E. Varner, K. I. Kim, and M. C. Nagan (2006)
Nucleic Acids Res. 34, 5361-5368
   Abstract »    Full Text »    PDF »
Structure of the 70S Ribosome Complexed with mRNA and tRNA.
M. Selmer, C. M. Dunham, F. V. Murphy IV, A. Weixlbaumer, S. Petry, A. C. Kelley, J. R. Weir, and V. Ramakrishnan (2006)
Science 313, 1935-1942
   Abstract »    Full Text »    PDF »
Evaluation of RNA-binding specificity of aminoglycosides with DNA microarrays.
F.-S. Liang, W. A. Greenberg, J. A. Hammond, J. Hoffmann, S. R. Head, and C.-H. Wong (2006)
PNAS 103, 12311-12316
   Abstract »    Full Text »    PDF »
Functional conformations of the L11-ribosomal RNA complex revealed by correlative analysis of cryo-EM and molecular dynamics simulations.
W. Li, J. Sengupta, B. K. Rath, and J. Frank (2006)
RNA 12, 1240-1253
   Abstract »    Full Text »    PDF »
Characterization of GE82832, a peptide inhibitor of translocation interacting with bacterial 30S ribosomal subunits.
L. Brandi, A. Fabbretti, M. D. Stefano, A. Lazzarini, M. Abbondi, and C. O. Gualerzi (2006)
RNA 12, 1262-1270
   Abstract »    Full Text »    PDF »
Inhibition of Human Tyrosyl-DNA Phosphodiesterase by Aminoglycoside Antibiotics and Ribosome Inhibitors.
Z. Liao, L. Thibaut, A. Jobson, and Y. Pommier (2006)
Mol. Pharmacol. 70, 366-372
   Abstract »    Full Text »    PDF »
Ribose 2'-hydroxyl groups in the 5' strand of the acceptor arm of P-site tRNA are not essential for EF-G catalyzed translocation.
J. S. FEINBERG and S. JOSEPH (2006)
RNA 12, 580-588
   Abstract »    Full Text »    PDF »
Structural and evolutionary classification of G/U wobble basepairs in the ribosome.
A. Mokdad, M. V. Krasovska, J. Sponer, and N. B. Leontis (2006)
Nucleic Acids Res. 34, 1326-1341
   Abstract »    Full Text »    PDF »
Specific functional interactions of nucleotides at key -3 and +4 positions flanking the initiation codon with components of the mammalian 48S translation initiation complex..
A. V. Pisarev, V. G. Kolupaeva, V. P. Pisareva, W. C. Merrick, C. U.T. Hellen, and T. V. Pestova (2006)
Genes & Dev. 20, 624-636
   Abstract »    Full Text »    PDF »
Two conformational states in the crystal structure of the Homo sapiens cytoplasmic ribosomal decoding A site.
J. Kondo, A. Urzhumtsev, and E. Westhof (2006)
Nucleic Acids Res. 34, 676-685
   Abstract »    Full Text »    PDF »
Two Distinct Conformations of the Conserved RNA-rich Decoding Center of the Small Ribosomal Subunit Are Recognized by tRNAs and Release Factors.
E.M. YOUNGMAN, L. COCHELLA, J.L. BRUNELLE, S. HE, and R. GREEN (2006)
Cold Spring Harb Symp Quant Biol 71, 545-549
   Abstract »    PDF »
Solution probing of metal ion binding by helix 27 from Escherichia coli 16S rRNA.
M. N. LAMBERT, J. A.H. HOERTER, M. J.B. PEREIRA, and N. G. WALTER (2005)
RNA 11, 1688-1700
   Abstract »    Full Text »    PDF »
Contribution of 16S rRNA nucleotides forming the 30S subunit A and P sites to translation in Escherichia coli.
N. M. ABDI and K. FREDRICK (2005)
RNA 11, 1624-1632
   Abstract »    Full Text »    PDF »
Rsp5 ubiquitin ligase modulates translation accuracy in yeast Saccharomyces cerevisiae.
M. KWAPISZ, P. CHOLBINSKI, A. K. HOPPER, J.-P. ROUSSET, and T. ZOLADEK (2005)
RNA 11, 1710-1718
   Abstract »    Full Text »    PDF »
Simulating movement of tRNA into the ribosome during decoding.
K. Y. Sanbonmatsu, S. Joseph, and C.-S. Tung (2005)
PNAS 102, 15854-15859
   Abstract »    Full Text »    PDF »
Crystal structures of complexes between aminoglycosides and decoding A site oligonucleotides: role of the number of rings and positive charges in the specific binding leading to miscoding.
B. Francois, R. J. M. Russell, J. B. Murray, F. Aboul-ela, B. Masquida, Q. Vicens, and E. Westhof (2005)
Nucleic Acids Res. 33, 5677-5690
   Abstract »    Full Text »    PDF »
RNA Structure: Reading the Ribosome.
H. F. Noller (2005)
Science 309, 1508-1514
   Abstract »    Full Text »    PDF »
Conformation of 4.5S RNA in the signal recognition particle and on the 30S ribosomal subunit.
S.-Q. GU, J. JOCKEL, P. BEINKER, J. WARNECKE, Y. P. SEMENKOV, M. V. RODNINA, and W. WINTERMEYER (2005)
RNA 11, 1374-1384
   Abstract »    Full Text »    PDF »
POPSCOMP: an automated interaction analysis of biomolecular complexes.
J. Kleinjung and F. Fraternali (2005)
Nucleic Acids Res. 33, W342-W346
   Abstract »    Full Text »    PDF »
A functional relationship between helix 1 and the 900 tetraloop of 16S ribosomal RNA within the bacterial ribosome.
F. BELANGER, G. THEBERGE-JULIEN, P. R. CUNNINGHAM, and L. BRAKIER-GINGRAS (2005)
RNA 11, 906-913
   Abstract »    Full Text »    PDF »
An Active Role for tRNA in Decoding Beyond Codon:Anticodon Pairing.
L. Cochella and R. Green (2005)
Science 308, 1178-1180
   Abstract »    Full Text »    PDF »
The N-terminal extension of Escherichia coli ribosomal protein L20 is important for ribosome assembly, but dispensable for translational feedback control.
M. GUILLIER, F. ALLEMAND, M. GRAFFE, S. RAIBAUD, F. DARDEL, M. SPRINGER, and C. CHIARUTTINI (2005)
RNA 11, 728-738
   Abstract »    Full Text »    PDF »
Initiation of Protein Synthesis in Bacteria.
B. S. Laursen, H. P. Sorensen, K. K. Mortensen, and H. U. Sperling-Petersen (2005)
Microbiol. Mol. Biol. Rev. 69, 101-123
   Abstract »    Full Text »    PDF »
A previously uncharacterized role for small protein B (SmpB) in transfer messenger RNA-mediated trans-translation.
T. R. Sundermeier, D. P. Dulebohn, H. J. Cho, and A. W. Karzai (2005)
PNAS 102, 2316-2321
   Abstract »    Full Text »    PDF »
The identification of novel RNA structural motifs using COMPADRES: an automated approach to structural discovery.
L. M. Wadley and A. M. Pyle (2004)
Nucleic Acids Res. 32, 6650-6659
   Abstract »    Full Text »    PDF »
A 16S rRNA-tRNA product containing a nucleotide phototrimer and specific for tRNA in the P/E hybrid state in the Escherichia coli ribosome.
W. Huggins and P. Wollenzien (2004)
Nucleic Acids Res. 32, 6548-6556
   Abstract »    Full Text »    PDF »
The driving force for molecular evolution of translation.
H. F. NOLLER (2004)
RNA 10, 1833-1837
   Abstract »    Full Text »    PDF »
Multiple defects in translation associated with altered ribosomal protein L4.
M. O'Connor, S. T. Gregory, and A. E. Dahlberg (2004)
Nucleic Acids Res. 32, 5750-5756
   Abstract »    Full Text »    PDF »
Photolabile anticodon stem-loop analogs of tRNAPhe as probes of ribosomal structure and structural fluctuation at the decoding center.
Z. DRUZINA and B. S. COOPERMAN (2004)
RNA 10, 1550-1562
   Abstract »    Full Text »    PDF »
A minimum structure of aminoglycosides that causes an initiation shift of trans-translation.
T. Konno, T. Takahashi, D. Kurita, A. Muto, and H. Himeno (2004)
Nucleic Acids Res. 32, 4119-4126
   Abstract »    Full Text »    PDF »
A reassessment of the response of the bacterial ribosome to the frameshift stimulatory signal of the human immunodeficiency virus type 1.
M. LEGER, S. SIDANI, and L. BRAKIER-GINGRAS (2004)
RNA 10, 1225-1235
   Abstract »    Full Text »    PDF »
Variable and conserved elements of human ribosomes surrounding the mRNA at the decoding and upstream sites.
D. Graifer, M. Molotkov, V. Styazhkina, N. Demeshkina, K. Bulygin, A. Eremina, A. Ivanov, E. Laletina, A. Ven'yaminova, and G. Karpova (2004)
Nucleic Acids Res. 32, 3282-3293
   Abstract »    Full Text »    PDF »
Mapping of the second tetracycline binding site on the ribosomal small subunit of E.coli.
M. M. Anokhina, A. Barta, K. H. Nierhaus, V. A. Spiridonova, and A. M. Kopylov (2004)
Nucleic Acids Res. 32, 2594-2597
   Abstract »    Full Text »    PDF »
tRNA slippage at the tmRNA resume codon.
M. J. TRIMBLE, A. MINNICUS, and K. P. WILLIAMS (2004)
RNA 10, 805-812
   Abstract »    Full Text »    PDF »
Three-dimensional motifs from the SCOR, structural classification of RNA database: extruded strands, base triples, tetraloops and U-turns.
P. S. Klosterman, D. K. Hendrix, M. Tamura, S. R. Holbrook, and S. E. Brenner (2004)
Nucleic Acids Res. 32, 2342-2352
   Abstract »    Full Text »    PDF »
Genetic evidence against the 16S ribosomal RNA helix 27 conformational switch model.
D. RODRIGUEZ-CORREA and A. E. DAHLBERG (2004)
RNA 10, 28-33
   Abstract »    Full Text »    PDF »
Purine bases at position 37 of tRNA stabilize codon-anticodon interaction in the ribosomal A site by stacking and Mg2+-dependent interactions.
A. L. KONEVEGA, N. G. SOBOLEVA, V. I. MAKHNO, Y. P. SEMENKOV, W. WINTERMEYER, M. V. RODNINA, and V. I. KATUNIN (2004)
RNA 10, 90-101
   Abstract »    Full Text »    PDF »
Roles of 5-substituents of tRNA wobble uridines in the recognition of purine-ending codons.
K. Takai and S. Yokoyama (2003)
Nucleic Acids Res. 31, 6383-6391
   Abstract »    Full Text »    PDF »
A Functional Interaction between Ribosomal Proteins S7 and S11 within the Bacterial Ribosome.
F. Robert and L. Brakier-Gingras (2003)
J. Biol. Chem. 278, 44913-44920
   Abstract »    Full Text »    PDF »
Structures of deacylated tRNA mimics bound to the E site of the large ribosomal subunit.
T. M. SCHMEING, P. B. MOORE, and T. A. STEITZ (2003)
RNA 9, 1345-1352
   Abstract »    Full Text »    PDF »
RNA structure comparison, motif search and discovery using a reduced representation of RNA conformational space.
C. M. Duarte, L. M. Wadley, and A. M. Pyle (2003)
Nucleic Acids Res. 31, 4755-4761
   Abstract »    Full Text »    PDF »
Plasmid-Mediated High-Level Resistance to Aminoglycosides in Enterobacteriaceae Due to 16S rRNA Methylation.
M. Galimand, P. Courvalin, and T. Lambert (2003)
Antimicrob. Agents Chemother. 47, 2565-2571
   Abstract »    Full Text »    PDF »
Translational Selection and Yeast Proteome Evolution.
H. Akashi (2003)
Genetics 164, 1291-1303
   Abstract »    Full Text »    PDF »
The GTPase Activity and C-terminal Cysteine of the Escherichia coli MnmE Protein Are Essential for Its tRNA Modifying Function.
L. Yim, M. Martinez-Vicente, M. Villarroya, C. Aguado, E. Knecht, and M.-E. Armengod (2003)
J. Biol. Chem. 278, 28378-28387
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)