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Science 27 April 2001:
Vol. 292. no. 5517, pp. 744 - 748
DOI: 10.1126/science.1058069
Prev | Table of Contents

Reports

Virus Maturation Involving Large Subunit Rotations and Local Refolding

J. F. Conway,12 W. R. Wikoff,3 N. Cheng,1 R. L. Duda,4 R. W. Hendrix,4 J. E. Johnson,3 A. C. Steven1*

Large-scale conformational changes transform viral precursors into infectious virions. The structure of bacteriophage HK97 capsid, Head-II, was recently solved by crystallography, revealing a catenated cross-linked topology. We have visualized its precursor, Prohead-II, by cryoelectron microscopy and modeled the conformational change by appropriately adapting Head-II. Rigid-body rotations (~40 degrees) cause switching to an entirely different set of interactions; in addition, two motifs undergo refolding. These changes stabilize the capsid by increasing the surface area buried at interfaces and bringing the cross-link-forming residues, initially ~40 angstroms apart, close together. The inner surface of Prohead-II is negatively charged, suggesting that the transition is triggered electrostatically by DNA packaging.

1 Laboratory of Structural Biology Research, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, MD 20892, USA.
2 Institut de Biologie Structurale-J.-P. Ebel, 41 rue Jules Horowitz, 38027 Grenoble, France.
3 Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
4 Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.
*   To whom correspondence should be addressed at Building 6, Room B2-34, 6 Center Drive MSC 2717, NIH, Bethesda, MD 20892-2717, USA. E-mail: alasdair_steven{at}nih.gov

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