Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 27 April 2001:
Vol. 292. no. 5517, pp. 740 - 743
DOI: 10.1126/science.1059399
Prev | Table of Contents | Next

Reports

Differentiation of Embryonic Stem Cell Lines Generated from Adult Somatic Cells by Nuclear Transfer

Teruhiko Wakayama,1*dagger Viviane Tabar,2 Ivan Rodriguez,1 Anthony C. F. Perry,1* Lorenz Studer,2dagger Peter Mombaerts1

Embryonic stem (ES) cells are fully pluripotent in that they can differentiate into all cell types, including gametes. We have derived 35 ES cell lines via nuclear transfer (ntES cell lines) from adult mouse somatic cells of inbred, hybrid, and mutant strains. ntES cells contributed to an extensive variety of cell types, including dopaminergic and serotonergic neurons in vitro and germ cells in vivo. Cloning by transfer of ntES cell nuclei could result in normal development of fertile adults. These studies demonstrate the full pluripotency of ntES cells.

1 The Rockefeller University, New York, NY 10021, USA.
2 Laboratory of Stem Cell and Tumor Biology, Neurosurgery and Cellular Biochemistry and Biophysics, Sloan Kettering, New York, NY 10021, USA.
*   Present address: Advanced Cell Technology, One Innovation Drive, Worcester, MA 01605, USA.

dagger    To whom correspondence should be addressed. E-mail: teru{at}advancedcell.com; studerl{at}mskcc.org

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
From the Cover: Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos.
M. Oda, S. Tanaka, Y. Yamazaki, H. Ohta, M. Iwatani, M. Suzuki, J. Ohgane, N. Hattori, R. Yanagimachi, T. Wakayama, et al. (2009)
PNAS 106, 16293-16297
   Abstract »    Full Text »    PDF »
Proteomics Analysis of Epithelial Cells Reprogrammed in Cell-free Extract.
E. Pewsey, C. Bruce, A. S. Georgiou, M. Jones, D. Baker, S. Y. Ow, P. C. Wright, C. K. Freberg, P. Collas, and A. Fazeli (2009)
Mol. Cell. Proteomics 8, 1401-1412
   Abstract »    Full Text »    PDF »
Assessment of the developmental competence of human somatic cell nuclear transfer embryos by oocyte morphology classification.
Y. Yu, Q. Mai, X. Chen, L. Wang, L. Gao, C. Zhou, and Q. Zhou (2009)
Hum. Reprod. 24, 649-657
   Abstract »    Full Text »    PDF »
Production of healthy cloned mice from bodies frozen at -20{degrees}C for 16 years.
S. Wakayama, H. Ohta, T. Hikichi, E. Mizutani, T. Iwaki, O. Kanagawa, and T. Wakayama (2008)
PNAS 105, 17318-17322
   Abstract »    Full Text »    PDF »
Generation of mice derived from embryonic stem cells using blastocysts of different developmental ages.
H. Ohta, Y. Sakaide, and T. Wakayama (2008)
Reproduction 136, 581-587
   Abstract »    Full Text »    PDF »
A comprehensive, non-invasive visualization of primordial germ cell development in mice by the Prdm1-mVenus and Dppa3-ECFP double transgenic reporter.
Y. Ohinata, M. Sano, M. Shigeta, K. Yamanaka, and M. Saitou (2008)
Reproduction 136, 503-514
   Abstract »    Full Text »    PDF »
Increasing the Cell Number of Host Tetraploid Embryos Can Improve the Production of Mice Derived from Embryonic Stem Cells.
H. Ohta, Y. Sakaide, K. Yamagata, and T. Wakayama (2008)
Biol Reprod 79, 486-492
   Abstract »    Full Text »    PDF »
Cell-Free Embryonic Stem Cell Extract-Mediated Derivation of Multipotent Stem Cells From NIH3T3 Fibroblasts for Functional and Anatomical Ischemic Tissue Repair.
J. Rajasingh, E. Lambers, H. Hamada, E. Bord, T. Thorne, I. Goukassian, P. Krishnamurthy, K. M. Rosen, D. Ahluwalia, Y. Zhu, et al. (2008)
Circ. Res. 102, e107-e117
   Abstract »    Full Text »    PDF »
Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors.
N. Takayama, H. Nishikii, J. Usui, H. Tsukui, A. Sawaguchi, T. Hiroyama, K. Eto, and H. Nakauchi (2008)
Blood 111, 5298-5306
   Abstract »    Full Text »    PDF »
Good manufacturing practice and clinical-grade human embryonic stem cell lines.
C. Unger, H. Skottman, P. Blomberg, M. Sirac Dilber, and O. Hovatta (2008)
Hum. Mol. Genet. 17, R48-R53
   Abstract »    Full Text »    PDF »
Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease.
M. Wernig, J.-P. Zhao, J. Pruszak, E. Hedlund, D. Fu, F. Soldner, V. Broccoli, M. Constantine-Paton, O. Isacson, and R. Jaenisch (2008)
PNAS 105, 5856-5861
   Abstract »    Full Text »    PDF »
Embryo development after successful somatic cell nuclear transfer to in vitro matured human germinal vesicle oocytes.
B. Heindryckx, P. De Sutter, J. Gerris, M. Dhont, and J. Van der Elst (2007)
Hum. Reprod. 22, 1982-1990
   Abstract »    Full Text »    PDF »
Contrasting Effects of in Vitro Fertilization and Nuclear Transfer on the Expression of mtDNA Replication Factors.
E. J. Bowles, J.-H. Lee, R. Alberio, R. E. I. Lloyd, D. Stekel, K. H. S. Campbell, and J. C. St. John (2007)
Genetics 176, 1511-1526
   Abstract »    Full Text »    PDF »
Epigenetic Reprogramming of OCT4 and NANOG Regulatory Regions by Embryonal Carcinoma Cell Extract.
C. T. Freberg, J. A. Dahl, S. Timoskainen, and P. Collas (2007)
Mol. Biol. Cell 18, 1543-1553
   Abstract »    Full Text »    PDF »
Over-expression of alpha-synuclein in human neural progenitors leads to specific changes in fate and differentiation.
B. L. Schneider, C. R. Seehus, E. E. Capowski, P. Aebischer, S.-C. Zhang, and C. N. Svendsen (2007)
Hum. Mol. Genet. 16, 651-666
   Abstract »    Full Text »    PDF »
Towards the Generation of Patient-Specific Pluripotent Stem Cells for Combined Gene and Cell Therapy of Hematologic Disorders.
G. Q. Daley (2007)
Hematology 2007, 17-22
   Abstract »    Full Text »    PDF »
Developmental ability of cloned embryos from neural stem cells..
E. Mizutani, H. Ohta, S. Kishigami, N. Van Thuan, T. Hikichi, S. Wakayama, M. Kosaka, E. Sato, and T. Wakayama (2006)
Reproduction 132, 849-857
   Abstract »    Full Text »    PDF »
Reprogramming somatic cells into stem cells..
R. Alberio, K. H Campbell, and A. D Johnson (2006)
Reproduction 132, 709-720
   Abstract »    Full Text »    PDF »
A comparative approach to somatic cell nuclear transfer in the rhesus monkey.
Q. Zhou, S.H. Yang, C.H. Ding, X.C. He, Y.H. Xie, T.B. Hildebrandt, S.M. Mitalipov, X.H. Tang, D.P. Wolf, and W.Z. Ji (2006)
Hum. Reprod. 21, 2564-2571
   Abstract »    Full Text »    PDF »
Heart muscle engineering: An update on cardiac muscle replacement therapy.
W.-H. Zimmermann, M. Didie, S. Doker, I. Melnychenko, H. Naito, C. Rogge, M. Tiburcy, and T. Eschenhagen (2006)
Cardiovasc Res 71, 419-429
   Abstract »    Full Text »    PDF »
Transmission of mitochondrial DNA following assisted reproduction and nuclear transfer.
E.C. Spikings, J. Alderson, and J.C.St. John (2006)
Hum. Reprod. Update 12, 401-415
   Abstract »    Full Text »    PDF »
Why the apparent haste to clone humans?.
N Cobbe (2006)
J. Med. Ethics 32, 298-302
   Full Text »    PDF »
Stem Cells as a Source of Regenerative Cardiomyocytes.
K. Fukuda and S. Yuasa (2006)
Circ. Res. 98, 1002-1013
   Abstract »    Full Text »    PDF »
Developmental abnormalities of NT mouse embryos appear early after implantation.
A. Jouneau, Q. Zhou, A. Camus, V. Brochard, L. Maulny, J. Collignon, and J.-P. Renard (2006)
Development 133, 1597-1607
   Abstract »    Full Text »    PDF »
Defined culture conditions of human embryonic stem cells.
J. Lu, R. Hou, C. J. Booth, S.-H. Yang, and M. Snyder (2006)
PNAS 103, 5688-5693
   Abstract »    Full Text »    PDF »
ES cells derived from cloned and fertilized blastocysts are transcriptionally and functionally indistinguishable.
T. Brambrink, K. Hochedlinger, G. Bell, and R. Jaenisch (2006)
PNAS 103, 933-938
   Abstract »    Full Text »    PDF »
Induction of Dedifferentiation, Genomewide Transcriptional Programming, and Epigenetic Reprogramming by Extracts of Carcinoma and Embryonic Stem Cells.
C. K. Taranger, A. Noer, A. L. Sorensen, A.-M. Hakelien, A. C. Boquest, and P. Collas (2005)
Mol. Biol. Cell 16, 5719-5735
   Abstract »    Full Text »    PDF »
Generation and Characterization of Pluripotent Stem Cells from Cloned Bovine Embryos.
L. Wang, E. Duan, L.-y. Sung, B.-S. Jeong, X. Yang, and X. C. Tian (2005)
Biol Reprod 73, 149-155
   Abstract »    Full Text »    PDF »
Embryonic stem cell differentiation: emergence of a new era in biology and medicine.
G. Keller (2005)
Genes & Dev. 19, 1129-1155
   Abstract »    Full Text »    PDF »
Establishment of Male and Female Nuclear Transfer Embryonic Stem Cell Lines from Different Mouse Strains and Tissues.
S. Wakayama, H. Ohta, S. Kishigami, N. Van Thuan, T. Hikichi, E. Mizutani, M. Miyake, and T. Wakayama (2005)
Biol Reprod 72, 932-936
   Abstract »    Full Text »    PDF »
From The Cover: Propagation of an infertile hermaphrodite mouse lacking germ cells by using nuclear transfer and embryonic stem cell technology.
S. Wakayama, S. Kishigami, N. Van Thuan, H. Ohta, T. Hikichi, E. Mizutani, R. Yanagimachi, and T. Wakayama (2005)
PNAS 102, 29-33
   Abstract »    Full Text »    PDF »
Inaugural Article: Nuclear cloning of embryonal carcinoma cells.
R. H. Blelloch, K. Hochedlinger, Y. Yamada, C. Brennan, M. Kim, B. Mintz, L. Chin, and R. Jaenisch (2004)
PNAS 101, 13985-13990
   Abstract »    Full Text »    PDF »
Somatic Cell Cloning: The Ultimate Form of Nuclear Reprogramming?.
J. A. Piedrahita, B. Mir, S. Dindot, and S. Walker (2004)
J. Am. Soc. Nephrol. 15, 1140-1144
   Abstract »    Full Text »    PDF »
Environmental guidance of normal and tumor cell plasticity: epithelial mesenchymal transitions as a paradigm.
G. Prindull and D. Zipori (2004)
Blood 103, 2892-2899
   Abstract »    Full Text »    PDF »
Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst.
W. S. Hwang, Y. J. Ryu, J. H. Park, E. S. Park, E. G. Lee, J. M. Koo, H. Y. Jeon, B. C. Lee, S. K. Kang, S. J. Kim, et al. (2004)
Science 303, 1669-1674
   Abstract »    Full Text »    PDF »
Enhanced Priming of Antigen-Specific CTLs In Vivo by Embryonic Stem Cell-Derived Dendritic Cells Expressing Chemokine Along with Antigenic Protein: Application to Antitumor Vaccination.
H. Matsuyoshi, S. Senju, S. Hirata, Y. Yoshitake, Y. Uemura, and Y. Nishimura (2004)
J. Immunol. 172, 776-786
   Abstract »    Full Text »    PDF »
Therapeutic cloning in the mouse.
P. Mombaerts (2003)
PNAS 100, 11924-11925
   Abstract »    Full Text »    PDF »
In vitro generation of T lymphocytes from embryonic stem cell-derived prehematopoietic progenitors.
R. F. de Pooter, S. K. Cho, J. R. Carlyle, and J. C. Zuniga-Pflucker (2003)
Blood 102, 1649-1653
   Abstract »    Full Text »    PDF »
Nuclear Transplantation, Embryonic Stem Cells, and the Potential for Cell Therapy.
K. Hochedlinger and R. Jaenisch (2003)
N. Engl. J. Med. 349, 275-286
   Full Text »    PDF »
Stem cells and neurological disease.
R A Barker, M Jain, R J E Armstrong, and M A Caldwell (2003)
J. Neurol. Neurosurg. Psychiatry 74, 553-557
   Full Text »    PDF »
Generation and genetic modification of dendritic cells derived from mouse embryonic stem cells.
S. Senju, S. Hirata, H. Matsuyoshi, M. Masuda, Y. Uemura, K. Araki, K.-i. Yamamura, and Y. Nishimura (2003)
Blood 101, 3501-3508
   Abstract »    Full Text »    PDF »
The youth revolution: cell biology in Japan.
W. A. Wells (2002)
J. Cell Biol. 158, 609-614
   Full Text »    PDF »
Stem Cells -- Scientific, Medical, and Political Issues.
I. L. Weissman (2002)
N. Engl. J. Med. 346, 1576-1579
   Full Text »    PDF »
From intestine to muscle: Nuclear reprogramming through defective cloned embryos.
J. A. Byrne, S. Simonsson, and J. B. Gurdon (2002)
PNAS 99, 6059-6063
   Abstract »    Full Text »    PDF »
Nuclear-Cytoplasmic "Tug of War" During Cloning: Effects of Somatic Cell Nuclei on Culture Medium Preferences of Preimplantation Cloned Mouse Embryos.
Y. G. Chung, M. R.W. Mann, M. S. Bartolomei, and K. E. Latham (2002)
Biol Reprod 66, 1178-1184
   Abstract »    Full Text »    PDF »
Placebo Surgery for Parkinson's Disease: Do the Benefits Outweigh the Risks?.
P. A. Clark (2002)
J. Law Med. Ethics 30, 58-68
   PDF »
From intestine to muscle: Nuclear reprogramming through defective cloned embryos.
J. A. Byrne, S. Simonsson, and J. B. Gurdon (2002)
PNAS 99, 6059-6063
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)