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Science 27 April 2001:
Vol. 292. no. 5517, pp. 740 - 743
DOI: 10.1126/science.1059399
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Reports

Differentiation of Embryonic Stem Cell Lines Generated from Adult Somatic Cells by Nuclear Transfer

Teruhiko Wakayama,1*dagger Viviane Tabar,2 Ivan Rodriguez,1 Anthony C. F. Perry,1* Lorenz Studer,2dagger Peter Mombaerts1

Embryonic stem (ES) cells are fully pluripotent in that they can differentiate into all cell types, including gametes. We have derived 35 ES cell lines via nuclear transfer (ntES cell lines) from adult mouse somatic cells of inbred, hybrid, and mutant strains. ntES cells contributed to an extensive variety of cell types, including dopaminergic and serotonergic neurons in vitro and germ cells in vivo. Cloning by transfer of ntES cell nuclei could result in normal development of fertile adults. These studies demonstrate the full pluripotency of ntES cells.

1 The Rockefeller University, New York, NY 10021, USA.
2 Laboratory of Stem Cell and Tumor Biology, Neurosurgery and Cellular Biochemistry and Biophysics, Sloan Kettering, New York, NY 10021, USA.
*   Present address: Advanced Cell Technology, One Innovation Drive, Worcester, MA 01605, USA.

dagger    To whom correspondence should be addressed. E-mail: teru{at}advancedcell.com; studerl{at}mskcc.org

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