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Originally published in Science Express on 1 February 2001
Science 9 March 2001:
Vol. 291. no. 5510, pp. 1965 - 1969
DOI: 10.1126/science.1057269
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Reports

Recovery of Infectious Ebola Virus from Complementary DNA: RNA Editing of the GP Gene and Viral Cytotoxicity

Viktor E. Volchkov,12* Valentina A. Volchkova,1 Elke Mühlberger,1 Larissa V. Kolesnikova,1 Michael Weik,1 Olga Dolnik,1 Hans-Dieter Klenk1

To study the mechanisms underlying the high pathogenicity of Ebola virus, we have established a system that allows the recovery of infectious virus from cloned cDNA and thus permits genetic manipulation. We created a mutant in which the editing site of the gene encoding envelope glycoprotein (GP) was eliminated. This mutant no longer expressed the nonstructural glycoprotein sGP. Synthesis of GP increased, but most of it accumulated in the endoplasmic reticulum as immature precursor. The mutant was significantly more cytotoxic than wild-type virus, indicating that cytotoxicity caused by GP is down-regulated by the virus through transcriptional RNA editing and expression of sGP.

1 Institut für Virologie, Philipps-Universität, Robert-Koch-Strasse 17, 35037 Marburg, Germany.
2 University Claude Bernard Lyon-1, Filovirus Laboratory, 21 Avenue Tony Garnier, 69365 Lyon Cedex 07, France.
*   To whom correspondence should be addressed. E-mail: viktor.volchkov{at}ens-lyon.fr

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