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Science 23 February 2001:
Vol. 291. no. 5508, pp. 1547 - 1550
DOI: 10.1126/science.1056866
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Reports

Requirement of a Centrosomal Activity for Cell Cycle Progression Through G1 into S Phase

Edward H. Hinchcliffe,1 Frederick J. Miller,1 Matthew Cham,1 Alexey Khodjakov,2 Greenfield Sluder1*

Centrosomes were microsurgically removed from BSC-1 African green monkey kidney cells before the completion of S phase. Karyoplasts (acentrosomal cells) entered and completed mitosis. However, postmitotic karyoplasts arrested before S phase, whereas adjacent control cells divided repeatedly. Postmitotic karyoplasts assembled a microtubule-organizing center containing gamma -tubulin and pericentrin, but did not regenerate centrioles. These observations reveal the existence of an activity associated with core centrosomal structures--distinct from elements of the microtubule-organizing center--that is required for the somatic cell cycle to progress through G1 into S phase. Once the cell is in S phase, these core structures are not needed for the G2-M phase transition.

1 Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
2 Laboratory of Cell Regulation, Wadsworth Center, Albany, NY 12201, USA.
*   To whom correspondence should be addressed. E-mail: greenfield.sluder{at}umassmed.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)