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Science 16 February 2001:
Vol. 291. no. 5507, pp. 1363 - 1365
DOI: 10.1126/science.291.5507.1363
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Tech.Sight

Worth Its Weight in Gold

Moise Bendayan

Immunocytochemistry, bridging morphology to biochemistry, has reached unsurpassed levels of sensitivity and resolution with the introduction of electron microscope markers using colloidal gold particles. The use of such markers has allowed for quantitative evaluations of antigens as well as multiple labelings of various antigens within the same cellular compartment. Other technological advances in tissue preparation techniques and molecular biology methods have significantly improved the versatility of immunocytochemistry. The advantages and some limitations of the new methods are discussed.

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Association of AMP-activated Protein Kinase Subunits With Glycogen Particles as Revealed In Situ by Immunoelectron Microscopy.
M. Bendayan, I. Londono, B. E. Kemp, G. D. Hardie, N. Ruderman, and M. Prentki (2009)
J. Histochem. Cytochem. 57, 963-971
   Abstract »    Full Text »    PDF »
A Stereological Approach for Estimation of Cellular Immunogold Labeling and Its Spatial Distribution in Oriented Sections Using the Rotator.
J. M. Lucocq and C. Gawden-Bone (2009)
J. Histochem. Cytochem. 57, 709-719
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)