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Science 15 December 2000:
Vol. 290. no. 5499, pp. 2095 - 2096
DOI: 10.1126/science.290.5499.2095
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Tech.Sight

MOLECULAR BIOLOGY:
Making Catalytic DNAs

Ronald R. Breaker

Although cells routinely use enzymes based on RNA and protein, catalytic DNAs are valuable enzymes that seem to have been ignored by Nature. Enzyme engineers, however, have no intention of ignoring catalytic DNAs (or deoxyribozymes as they are known) and are busy synthesizing them in the laboratory and finding applications for them in biotechnology and therapeutics, as Ronald Breaker reveals in his Techview article.

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Origin of Informational Polymers: DIFFERENTIAL STABILITY OF PHOSPHOESTER BONDS IN RIBOMONOMERS AND RIBOOLIGOMERS.
R. Saladino, C. Crestini, F. Ciciriello, E. Di Mauro, and G. Costanzo (2006)
J. Biol. Chem. 281, 5790-5796
   Abstract »    Full Text »    PDF »
Characterization of a catalytically efficient acidic RNA-cleaving deoxyribozyme.
S. A. Kandadai and Y. Li (2006)
Nucleic Acids Res. 33, 7164-7175
   Abstract »    Full Text »    PDF »
Down-regulation of Plasminogen Activator Inhibitor 1 Expression Promotes Myocardial Neovascularization by Bone Marrow Progenitors.
G. Xiang, M. D. Schuster, T. Seki, A. A. Kocher, S. Eshghi, A. Boyle, and S. Itescu (2004)
J. Exp. Med. 200, 1657-1666
   Abstract »    Full Text »    PDF »
A novel replicating circular DNAzyme.
F. Chen, R. Wang, Z. Li, B. Liu, X. Wang, Y. Sun, D. Hao, and J. Zhang (2004)
Nucleic Acids Res. 32, 2336-2341
   Abstract »    Full Text »    PDF »
DNAzymes to beta 1 and beta 3 mRNA Down-regulate Expression of the Targeted Integrins and Inhibit Endothelial Cell Capillary Tube Formation in Fibrin and Matrigel.
M. Cieslak, J. Niewiarowska, M. Nawrot, M. Koziolkiewicz, W. J. Stec, and C. S. Cierniewski (2002)
J. Biol. Chem. 277, 6779-6787
   Abstract »    Full Text »    PDF »
The reactivity of phosphodiester bonds within linear single-stranded oligoribonucleotides is strongly dependent on the base sequence.
U. Kaukinen, S. Lyytikainen, S. Mikkola, and H. Lonnberg (2002)
Nucleic Acids Res. 30, 468-474
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)