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Science 3 November 2000:
Vol. 290. no. 5493, pp. 979 - 982
DOI: 10.1126/science.290.5493.979
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Reports

VirB/D4-Dependent Protein Translocation from Agrobacterium into Plant Cells

Annette C. Vergunst, Barbara Schrammeijer, Amke den Dulk-Ras, Clementine M. T. de Vlaam, Tonny J. G. Regensburg-Tuïnk, Paul J. J. Hooykaas*

The Agrobacterium VirB/D4 transport system mediates the transfer of a nucleoprotein T complex into plant cells, leading to crown gall disease. In addition, several Virulence proteins must somehow be transported to fulfill a function in planta. Here, we used fusions between Cre recombinase and VirE2 or VirF to directly demonstrate protein translocation into plant cells. Transport of the proteins was monitored by a Cre-mediated in planta recombination event resulting in a selectable phenotype and depended on the VirB/D4 transport system but did not require transferred DNA.

Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL, Leiden, Netherlands.
*   To whom correspondence should be addressed. E-mail: hooykaas{at}rulbim.leidenuniv.nl

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