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Science 27 October 2000:
Vol. 290. no. 5492, pp. 725 - 726
DOI: 10.1126/science.290.5492.725
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Perspectives

SIGNAL TRANSDUCTION:
N-WASP Regulation--the Sting in the Tail

James Fawcett and Tony Pawson

Signaling proteins can be regulated by their interactions with other proteins and phospholipids. As Fawcett and Pawson discuss in their Perspective, activation of the N-WASP protein (which coordinates formation of actin filaments) is far more complex, depending on the interaction of N-WASP with both a protein and a phospholipid. The authors explain new results (Prehoda et al.) demonstrating that cooperative binding of the phospholipid PIP2 and the small GTPase Cdc42 to N-WASP results in its activation. The Arp2/3 complex is then able to bind to N-WASP and to proceed with its job of initiating the assembly of actin monomers into actin filaments.

The authors are at the Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto, Ontario M5G 1X5, Canada, and the Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5G 1X5, Canada. E-mail: fawcett{at}mshri.on.ca, pawson{at}mshri.on.ca

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