Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 20 October 2000:
Vol. 290. no. 5491, pp. 527 - 530
DOI: 10.1126/science.290.5491.527
Prev | Table of Contents | Next

Reports

CD95/CD95 Ligand Interactions on Epithelial Cells in Host Defense to Pseudomonas aeruginosa

Heike Grassmé,1 Susanne Kirschnek,1 Joachim Riethmueller,1 Andrea Riehle,1 Gabriele von Kürthy,2 Florian Lang,1 Michael Weller,2 Erich Gulbins1*dagger

Pseudomonas aeruginosa causes severe infections, particularly of the lung, that are life threatening. Here, we show that P. aeruginosa infection induces apoptosis of lung epithelial cells by activation of the endogenous CD95/CD95 ligand system. Deficiency of CD95 or CD95 ligand on epithelial cells prevented apoptosis of lung epithelial cells in vivo as well as in vitro. The importance of CD95/CD95 ligand-mediated lung epithelial cell apoptosis was demonstrated by the rapid development of sepsis in CD95- or CD95 ligand-deficient mice, but not in normal mice, after P. aeruginosa infection.

1 Department of Physiology, University of Tuebingen, Gmelinstrasse 5, 72076 Tuebingen, Germany.
2 Department of Neurology, University of Tuebingen, Hoppe-Seyler Strasse 3, 72076 Tuebingen, Germany.
*   Present address: Department of Immunology, St. Jude Children's Research Hospital, 332 North Lauderdale Street, Memphis, TN 38105, USA.

dagger    To whom correspondence should be addressed. E-mail: erich.gulbins{at}stjude.org

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Role of Twitching Motility in Pseudomonas aeruginosa Exit from and Translocation of Corneal Epithelial Cells.
I. Alarcon, D. J. Evans, and S. M. J. Fleiszig (2009)
Invest. Ophthalmol. Vis. Sci. 50, 2237-2244
   Abstract »    Full Text »    PDF »
Leptospira interrogans Induces Apoptosis in Macrophages via Caspase-8- and Caspase-3-Dependent Pathways.
D. Jin, D. M. Ojcius, D. Sun, H. Dong, Y. Luo, Y. Mao, and J. Yan (2009)
Infect. Immun. 77, 799-809
   Abstract »    Full Text »    PDF »
Sphingolipids in the Lungs.
S. Uhlig and E. Gulbins (2008)
Am. J. Respir. Crit. Care Med. 178, 1100-1114
   Abstract »    Full Text »    PDF »
Animal models of chronic lung infection with Pseudomonas aeruginosa: useful tools for cystic fibrosis studies.
I Kukavica-Ibrulj and R C Levesque (2008)
Lab Anim 42, 389-412
   Abstract »    Full Text »    PDF »
Substance P Delays Apoptosis, Enhancing Keratitis after Pseudomonas aeruginosa Infection.
Z. Zhou, R. P. Barrett, S. A. McClellan, Y. Zhang, E. A. Szliter, N. van Rooijen, and L. D. Hazlett (2008)
Invest. Ophthalmol. Vis. Sci. 49, 4458-4467
   Abstract »    Full Text »    PDF »
Acid Sphingomyelinase Amplifies Redox Signaling in Pseudomonas aeruginosa-Induced Macrophage Apoptosis.
Y. Zhang, X. Li, A. Carpinteiro, and E. Gulbins (2008)
J. Immunol. 181, 4247-4254
   Abstract »    Full Text »    PDF »
Bacteria in the Intestine, Helpful Residents or Enemies from Within?.
G. O. Canny and B. A. McCormick (2008)
Infect. Immun. 76, 3360-3373
   Full Text »    PDF »
Immune recognition of Pseudomonas aeruginosa mediated by the IPAF/NLRC4 inflammasome.
F. S. Sutterwala, L. A. Mijares, L. Li, Y. Ogura, B. I. Kazmierczak, and R. A. Flavell (2007)
J. Exp. Med. 204, 3235-3245
   Abstract »    Full Text »    PDF »
Individual Matrix Metalloproteinases Control Distinct Transcriptional Responses in Airway Epithelial Cells Infected with Pseudomonas aeruginosa.
S. Y. Kassim, S. A. Gharib, B. H. Mecham, T. P. Birkland, W. C. Parks, and J. K. McGuire (2007)
Infect. Immun. 75, 5640-5650
   Abstract »    Full Text »    PDF »
Fas-Negative Osteosarcoma Tumor Cells Are Selected during Metastasis to the Lungs: The Role of the Fas Pathway in the Metastatic Process of Osteosarcoma.
N. V. Koshkina, C. Khanna, A. Mendoza, H. Guan, L. DeLauter, and E. S. Kleinerman (2007)
Mol. Cancer Res. 5, 991-999
   Abstract »    Full Text »    PDF »
Mechanisms and Significance of Cell Volume Regulation.
F. Lang (2007)
J. Am. Coll. Nutr. 26, 613S-623S
   Abstract »    Full Text »    PDF »
Host Resistance to Lung Infection Mediated by Major Vault Protein in Epithelial Cells.
M. P. Kowalski, A. Dubouix-Bourandy, M. Bajmoczi, D. E. Golan, T. Zaidi, Y. S. Coutinho-Sledge, M. P. Gygi, S. P. Gygi, E. A. C. Wiemer, and G. B. Pier (2007)
Science 317, 130-132
   Abstract »    Full Text »    PDF »
Expression of Pseudomonas aeruginosa Toxin ExoS Effectively Induces Apoptosis in Host Cells.
J. Jia, Y. Wang, L. Zhou, and S. Jin (2006)
Infect. Immun. 74, 6557-6570
   Abstract »    Full Text »    PDF »
Pseudomonas aeruginosa-Induced Human Mast Cell Apoptosis Is Associated with Up-Regulation of Endogenous Bcl-xS and Down-Regulation of Bcl-xL.
C. E. Jenkins, A. Swiatoniowski, M. R. Power, and T.-J. Lin (2006)
J. Immunol. 177, 8000-8007
   Abstract »    Full Text »    PDF »
Irradiation-Induced Pneumonitis Mediated by the CD95/CD95-Ligand System..
F. Heinzelmann, V. Jendrossek, K. Lauber, K. Nowak, T. Eldh, R. Boras, R. Handrick, M. Henkel, C. Martin, S. Uhlig, et al. (2006)
J Natl Cancer Inst 98, 1248-1251
   Abstract »    Full Text »    PDF »
Uropathogenic Escherichia coli Induces Extrinsic and Intrinsic Cascades To Initiate Urothelial Apoptosis.
D. J. Klumpp, M. T. Rycyk, M. C. Chen, P. Thumbikat, S. Sengupta, and A. J. Schaeffer (2006)
Infect. Immun. 74, 5106-5113
   Abstract »    Full Text »    PDF »
The human cationic host defense peptide LL-37 mediates contrasting effects on apoptotic pathways in different primary cells of the innate immune system.
P. G. Barlow, Y. Li, T. S. Wilkinson, D. M. E. Bowdish, Y. E. Lau, C. Cosseau, C. Haslett, A. J. Simpson, R. E. W. Hancock, and D. J. Davidson (2006)
J. Leukoc. Biol. 80, 509-520
   Abstract »    Full Text »    PDF »
The NKG2D-Activating Receptor Mediates Pulmonary Clearance of Pseudomonas aeruginosa..
M. T. Borchers, N. L. Harris, S. C. Wesselkamper, S. Zhang, Y. Chen, L. Young, and G. W. Lau (2006)
Infect. Immun. 74, 2578-2586
   Abstract »    Full Text »    PDF »
Targeted Immunomodulation of the NF-{kappa}B Pathway in Airway Epithelium Impacts Host Defense against Pseudomonas aeruginosa..
R. T. Sadikot, H. Zeng, M. Joo, M. B. Everhart, T. P. Sherrill, B. Li, D.-s. Cheng, F. E. Yull, J. W. Christman, and T. S. Blackwell (2006)
J. Immunol. 176, 4923-4930
   Abstract »    Full Text »    PDF »
Apoptosis of Airway Epithelial Cells: Human Serum Sensitive Induction by the Cathelicidin LL-37.
Y. E. Lau, D. M. E. Bowdish, C. Cosseau, R. E. W. Hancock, and D. J. Davidson (2006)
Am. J. Respir. Cell Mol. Biol. 34, 399-409
   Abstract »    Full Text »    PDF »
Phospholipase A2 Functions in Pseudomonas aeruginosa- Induced Apoptosis.
S. Kirschnek and E. Gulbins (2006)
Infect. Immun. 74, 850-860
   Abstract »    Full Text »    PDF »
Physiological and pathophysiological aspects of ceramide.
E. Gulbins and P. L. Li (2006)
Am J Physiol Regulatory Integrative Comp Physiol 290, R11-R26
   Abstract »    Full Text »    PDF »
Ceramide mediates caspase-independent programmed cell death.
L. Thon, H. Mohlig, S. Mathieu, A. Lange, E. Bulanova, S. Winoto-Morbach, S. Schutze, S. Bulfone-Paus, and D. Adam (2005)
FASEB J 19, 1945-1956
   Abstract »    Full Text »    PDF »
Reprogramming of circulatory cells in sepsis and SIRS.
J.-M. Cavaillon, C. Adrie, C. Fitting, and M. Adib-Conquy (2005)
Innate Immunity 11, 311-320
   Abstract »    PDF »
Apoptosis and Epithelial Injury in the Lungs.
T. R. Martin, N. Hagimoto, M. Nakamura, and G. Matute-Bello (2005)
Proceedings of the ATS 2, 214-220
   Abstract »    Full Text »    PDF »
NF-{kappa}B Regulates Fas-Mediated Apoptosis in HIV-Associated Nephropathy.
M. J. Ross, S. Martinka, V. D. D'Agati, and L. A. Bruggeman (2005)
J. Am. Soc. Nephrol. 16, 2403-2411
   Abstract »    Full Text »    PDF »
Pathogen-Host Interactions in Pseudomonas aeruginosa Pneumonia.
R. T. Sadikot, T. S. Blackwell, J. W. Christman, and A. S. Prince (2005)
Am. J. Respir. Crit. Care Med. 171, 1209-1223
   Abstract »    Full Text »    PDF »
The Phosphoinositol-3-Kinase-Protein Kinase B/Akt Pathway Is Critical for Pseudomonas aeruginosa Strain PAK Internalization.
A. Kierbel, A. Gassama-Diagne, K. Mostov, and J. N. Engel (2005)
Mol. Biol. Cell 16, 2577-2585
   Abstract »    Full Text »    PDF »
Internalization of Pseudomonas aeruginosa Is Mediated by Lipid Rafts in Contact Lens-Wearing Rabbit and Cultured Human Corneal Epithelial Cells.
N. Yamamoto, N. Yamamoto, M. W. Petroll, H. D. Cavanagh, and J. V. Jester (2005)
Invest. Ophthalmol. Vis. Sci. 46, 1348-1355
   Abstract »    Full Text »    PDF »
Pyocyanin Production by Pseudomonas aeruginosa Induces Neutrophil Apoptosis and Impairs Neutrophil-Mediated Host Defenses In Vivo.
L. Allen, D. H. Dockrell, T. Pattery, D. G. Lee, P. Cornelis, P. G. Hellewell, and M. K. B. Whyte (2005)
J. Immunol. 174, 3643-3649
   Abstract »    Full Text »    PDF »
Relationship of Acute Lung Inflammatory Injury to Fas/FasL System.
T. A. Neff, R.-F. Guo, S. B. Neff, J. V. Sarma, C. L. Speyer, H. Gao, K. D. Bernacki, M. Huber-Lang, S. McGuire, L. M. Hoesel, et al. (2005)
Am. J. Pathol. 166, 685-694
   Abstract »    Full Text »    PDF »
Pseudomonas aeruginosa Flagellin and Alginate Elicit Very Distinct Gene Expression Patterns in Airway Epithelial Cells: Implications for Cystic Fibrosis Disease.
L. M. Cobb, J. C. Mychaleckyj, D. J. Wozniak, and Y. S. Lopez-Boado (2004)
J. Immunol. 173, 5659-5670
   Abstract »    Full Text »    PDF »
Heme Oxygenase-1 Expression in Human Lungs with Cystic Fibrosis and Cytoprotective Effects against Pseudomonas Aeruginosa In Vitro.
H. Zhou, F. Lu, C. Latham, D. S. Zander, and G. A. Visner (2004)
Am. J. Respir. Crit. Care Med. 170, 633-640
   Abstract »    Full Text »    PDF »
Streptococcus pneumoniae-Induced Caspase 6-Dependent Apoptosis in Lung Epithelium.
B. Schmeck, R. Gross, P. D. N'Guessan, A. C. Hocke, S. Hammerschmidt, T. J. Mitchell, S. Rosseau, N. Suttorp, and S. Hippenstiel (2004)
Infect. Immun. 72, 4940-4947
   Abstract »    Full Text »    PDF »
Pseudomonas aeruginosa Exotoxin A Induces Human Mast Cell Apoptosis by a Caspase-8 and -3-dependent Mechanism.
C. E. Jenkins, A. Swiatoniowski, A. C. Issekutz, and T.-J. Lin (2004)
J. Biol. Chem. 279, 37201-37207
   Abstract »    Full Text »    PDF »
Proapoptotic Effect of Proteolytic Activation of Matrix Metalloproteinases by Streptococcus pyogenes Thiol Proteinase (Streptococcus Pyrogenic Exotoxin B).
F. Tamura, R. Nakagawa, T. Akuta, S. Okamoto, S. Hamada, H. Maeda, S. Kawabata, and T. Akaike (2004)
Infect. Immun. 72, 4836-4847
   Abstract »    Full Text »    PDF »
Role of EGFR Transactivation in Preventing Apoptosis in Pseudomonas aeruginosa-Infected Human Corneal Epithelial Cells.
J. Zhang, H. Li, J. Wang, Z. Dong, S. Mian, and F.-S. X. Yu (2004)
Invest. Ophthalmol. Vis. Sci. 45, 2569-2576
   Abstract »    Full Text »    PDF »
Localization of Cystic Fibrosis Transmembrane Conductance Regulator to Lipid Rafts of Epithelial Cells Is Required for Pseudomonas aeruginosa-Induced Cellular Activation.
M. P. Kowalski and G. B. Pier (2004)
J. Immunol. 172, 418-425
   Abstract »    Full Text »    PDF »
Alveolar Macrophage Apoptosis Contributes to Pneumococcal Clearance in a Resolving Model of Pulmonary Infection.
D. H. Dockrell, H. M. Marriott, L. R. Prince, V. C. Ridger, P. G. Ince, P. G. Hellewell, and M. K. B. Whyte (2003)
J. Immunol. 171, 5380-5388
   Abstract »    Full Text »    PDF »
Pseudomonas aeruginosa-Induced Apoptosis Is Defective in Respiratory Epithelial Cells Expressing Mutant Cystic Fibrosis Transmembrane Conductance Regulator.
C. L. Cannon, M. P. Kowalski, K. S. Stopak, and G. B. Pier (2003)
Am. J. Respir. Cell Mol. Biol. 29, 188-197
   Abstract »    Full Text »    PDF »
c-Jun NH2-Terminal Kinase-Mediated Signaling Is Essential for Pseudomonas aeruginosa ExoS-Induced Apoptosis.
J. Jia, M. Alaoui-El-Azher, M. Chow, T. C. Chambers, H. Baker, and S. Jin (2003)
Infect. Immun. 71, 3361-3370
   Abstract »    Full Text »    PDF »
Apoptotic Response of Chang Cells to Infection with Pseudomonas aeruginosa Strains PAK and PAO-I: Molecular Ordering of the Apoptosis Signaling Cascade and Role of Type IV Pili.
V. Jendrossek, S. Fillon, C. Belka, I. Muller, B. Puttkammer, and F. Lang (2003)
Infect. Immun. 71, 2665-2673
   Abstract »    Full Text »    PDF »
Delta 9-Tetrahydrocannabinol disrupts mitochondrial function and cell energetics.
T. A. Sarafian, S. Kouyoumjian, F. Khoshaghideh, D. P. Tashkin, and M. D. Roth (2003)
Am J Physiol Lung Cell Mol Physiol 284, L298-L306
   Abstract »    Full Text »    PDF »
Cutting Edge: Salmonella AvrA Effector Inhibits the Key Proinflammatory, Anti-Apoptotic NF-{kappa}B Pathway.
L. S. Collier-Hyams, H. Zeng, J. Sun, A. D. Tomlinson, Z. Q. Bao, H. Chen, J. L. Madara, K. Orth, and A. S. Neish (2002)
J. Immunol. 169, 2846-2850
   Abstract »    Full Text »    PDF »
Tumor Necrosis Factor-{alpha} and Angiostatin Are Mediators of Endothelial Cytotoxicity in Bronchoalveolar Lavages of Patients with Acute Respiratory Distress Syndrome.
J. Hamacher, R. Lucas, H. R. Lijnen, S. Buschke, Y. Dunant, A. Wendel, G. E. Grau, P. M. Suter, and B. Ricou (2002)
Am. J. Respir. Crit. Care Med. 166, 651-656
   Abstract »    Full Text »    PDF »
Enhanced Disease Severity in Helicobacter pylori-Infected Mice Deficient in Fas Signaling.
N. L. Jones, A. S. Day, H. Jennings, P. T. Shannon, E. Galindo-Mata, and P. M. Sherman (2002)
Infect. Immun. 70, 2591-2597
   Abstract »    Full Text »    PDF »
Early Mitochondrial Dysfunction, Superoxide Anion Production, and DNA Degradation Are Associated with Non-Apoptotic Death of Human Airway Epithelial Cells Induced by Pseudomonas aeruginosa Exotoxin A.
M.-C. Plotkowski, H. C. C. Povoa, J.-M. Zahm, G. Lizard, G. M. B. Pereira, J.-M. Tournier, and E. Puchelle (2002)
Am. J. Respir. Cell Mol. Biol. 26, 617-626
   Abstract »    Full Text »    PDF »
Lung Infections Associated with Cystic Fibrosis.
J. B. Lyczak, C. L. Cannon, and G. B. Pier (2002)
Clin. Microbiol. Rev. 15, 194-222
   Abstract »    Full Text »    PDF »
Persistent and aggressive bacteria in the lungs of cystic fibrosis children.
C A. Hart and C. Winstanley (2002)
Br. Med. Bull. 61, 81-96
   Abstract »    Full Text »    PDF »
Pseudomonas-Epithelial Cell Interactions Dissected With DNA Microarrays.
S. Lory and J. K. Ichikawa (2002)
Chest 121 , 36S-40S
   Abstract »    Full Text »    PDF »
Suppression of Akt Signaling Induces Fas Ligand Expression: Involvement of Caspase and Jun Kinase Activation in Akt-Mediated Fas Ligand Regulation.
T. Suhara, H.-S. Kim, L. A. Kirshenbaum, and K. Walsh (2002)
Mol. Cell. Biol. 22, 680-691
   Abstract »    Full Text »    PDF »
Immunity, Inflammation, and Remodeling in the Airway Epithelial Barrier: Epithelial-Viral-Allergic Paradigm.
M. J. Holtzman, J. D. Morton, L. P. Shornick, J. W. Tyner, M. P. O'Sullivan, A. Antao, M. Lo, M. Castro, and M. J. Walter (2002)
Physiol Rev 82, 19-46
   Abstract »    Full Text »    PDF »
Phosphatidylinositol 3-Kinase-dependent Pathways Oppose Fas-induced Apoptosis and Limit Chloride Secretion in Human Intestinal Epithelial Cells. IMPLICATIONS FOR INFLAMMATORY DIARRHEAL STATES.
M. T. Abreu, E. T. Arnold, J. Y. C. Chow, and K. E. Barrett (2001)
J. Biol. Chem. 276, 47563-47574
   Abstract »    Full Text »    PDF »
Reactive Oxygen Species and Cell Signaling.
J. R. Hoidal (2001)
Am. J. Respir. Cell Mol. Biol. 25, 661-663
   Full Text »    PDF »
Role of Apoptosis in Pseudomonas aeruginosa Pneumonia.
R. S. Hotchkiss, W. M. Dunne, P. E. Swanson, C. G. Davis, K. W. Tinsley, K. C. Chang, T. G. Buchman, I. E. Karl, H. Grassme, S. Kirschnek, et al. (2001)
Science 294, 1783a-1783
   Full Text »    PDF »
{alpha}-Toxin is a mediator of Staphylococcus aureus-induced cell death and activates caspases via the intrinsic death pathway independently of death receptor signaling.
H. Bantel, B. Sinha, W. Domschke, G. Peters, K. Schulze-Osthoff, and R. U. Janicke (2001)
J. Cell Biol. 155, 637-648
   Abstract »    Full Text »    PDF »
CD40 activation-induced, Fas-dependent apoptosis and NF-{kappa}B/AP-1 signaling in human intrahepatic biliary epithelial cells.
S. C. AFFORD, J. AHMED-CHOUDHURY, S. RANDHAWA, C. RUSSELL, J. YOUSTER, H. A. CROSBY, A. ELIOPOULOS, S. G. HUBSCHER, L. S. YOUNG, and D. H. ADAMS (2001)
FASEB J 15, 2345-2354
   Abstract »    Full Text »    PDF »
Fas/Fas Ligand System Mediates Epithelial Injury, but Not Pulmonary Host Defenses, in Response to Inhaled Bacteria.
G. Matute-Bello, C. W. Frevert, W. C. Liles, M. Nakamura, J. T. Ruzinski, K. Ballman, V. A. Wong, C. Vathanaprida, and T. R. Martin (2001)
Infect. Immun. 69, 5768-5776
   Abstract »    Full Text »    PDF »
Sepsis-Induced Apoptosis Causes Progressive Profound Depletion of B and CD4+ T Lymphocytes in Humans.
R. S. Hotchkiss, K. W. Tinsley, P. E. Swanson, R. E. Schmieg Jr., J. J. Hui, K. C. Chang, D. F. Osborne, B. D. Freeman, J. P. Cobb, T. G. Buchman, et al. (2001)
J. Immunol. 166, 6952-6963
   Abstract »    Full Text »    PDF »
Pseudomonas aeruginosa-Induced Apoptosis Involves Mitochondria and Stress-Activated Protein Kinases.
V. Jendrossek, H. Grassme, I. Mueller, F. Lang, and E. Gulbins (2001)
Infect. Immun. 69, 2675-2683
   Abstract »    Full Text »    PDF »
Programmed cell death mediated by ced-3 and ced-4 protects Caenorhabditis elegans from Salmonella typhimurium-mediated killing.
A. Aballay and F. M. Ausubel (2001)
PNAS
   Abstract »    Full Text »
CD95 Signaling via Ceramide-rich Membrane Rafts.
H. Grassme, A. Jekle, A. Riehle, H. Schwarz, J. Berger, K. Sandhoff, R. Kolesnick, and E. Gulbins (2001)
J. Biol. Chem. 276, 20589-20596
   Abstract »    Full Text »    PDF »
Programmed cell death mediated by ced-3 and ced-4 protects Caenorhabditis elegans from Salmonella typhimurium-mediated killing.
A. Aballay and F. M. Ausubel (2001)
PNAS 98, 2735-2739
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)