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Science 13 October 2000:
Vol. 290. no. 5490, pp. 337 - 341
DOI: 10.1126/science.290.5490.337
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Reports

A Myosin I Isoform in the Nucleus

Lidija Pestic-Dragovich,1* Ljuba Stojiljkovic,1* Anatoly A. Philimonenko,2 Grzegorz Nowak,1 Yunbo Ke,1 Robert E. Settlage,3 Jeffrey Shabanowitz,3 Donald F. Hunt,4 Pavel Hozak,2 Primal de Lanerolle1dagger

A nuclear isoform of myosin I beta  that contains a unique 16-amino acid amino-terminal extension has been identified. An affinity-purified antibody to the 16-amino acid peptide demonstrated nuclear staining. Confocal and electron microscopy revealed that nuclear myosin I beta  colocalized with RNA polymerase II in an alpha -amanitin- and actinomycin D-sensitive manner. The antibody coimmunoprecipitated RNA polymerase II and blocked in vitro RNA synthesis. This isoform of myosin I beta  appears to be in a complex with RNA polymerase II and may affect transcription.

1 Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL 60612, USA.
2 Department of Cell Ultrastructure and Molecular Biology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
3 Chemistry Department, University of Virginia, Charlottesville, VA 22901, USA.
4 Departments of Chemistry and Pathology, University of Virginia, Charlottesville, VA 22901, USA.
*   These authors contributed equally to this paper.

dagger    To whom correspondence should be addressed. E-mail: primal{at}uic.edu

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