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Science 29 September 2000:
Vol. 289. no. 5488, pp. 2357 - 2360
DOI: 10.1126/science.289.5488.2357
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Reports

Ubiquitin-Activating/Conjugating Activity of TAFII250, a Mediator of Activation of Gene Expression in Drosophila

Anh-Dung Pham, Frank Sauer*

Ubiquitination of histones has been linked to the complex processes that regulate the activation of eukaryotic transcription. However, the cellular factors that interpose this histone modification during the processes of transcriptional activation are not well characterized. A biochemical approach identified the Drosophila coactivator TAFII250, the central subunit within the general transcription factor TFIID, as a histone-specific ubiquitin-activating/conjugating enzyme (ubac). TAFII250 mediates monoubiquitination of histone H1 in vitro. Point mutations within the putative ubac domain of TAFII250 abolished H1-specific ubiquitination in vitro. In the Drosophila embryo, inactivation of the TAFII250 ubac activity reduces the cellular level of monoubiquitinated histone H1 and the expression of genes targeted by the maternal activator Dorsal. Thus, coactivator-mediated ubiquitination of proteins within the transactivation pathway may contribute to the processes directing activation of eukaryotic transcription.

Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
*   To whom correspondence should be addressed. E-mail: f.sauer{at}mail.zmbh.uni-heidelberg.de

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