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Science 29 September 2000:
Vol. 289. no. 5488, pp. 2354 - 2356
DOI: 10.1126/science.289.5488.2354
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Reports

A Specificity-Enhancing Factor for the ClpXP Degradation Machine

Igor Levchenko,12 Meredith Seidel,12 Robert T. Sauer,1 Tania A. Baker12*

Events that stall bacterial protein synthesis activate the ssrA-tagging machinery, resulting in resumption of translation and addition of an 11-residue peptide to the carboxyl terminus of the nascent chain. This ssrA-encoded peptide tag marks the incomplete protein for degradation by the energy-dependent ClpXP protease. Here, a ribosome-associated protein, SspB, was found to bind specifically to ssrA-tagged proteins and to enhance recognition of these proteins by ClpXP. Cells with an sspB mutation are defective in degrading ssrA-tagged proteins, demonstrating that SspB is a specificity-enhancing factor for ClpXP that controls substrate choice.

1 Department of Biology and
2 Howard Hughes Medical Institute, Building 68, Room 523, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
*   To whom correspondence should be addressed. E-mail: tabaker{at}mit.edu

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