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Science 8 September 2000:
Vol. 289. no. 5485, pp. 1760 - 1763
DOI:

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Reports

Printing Proteins as Microarrays for High-Throughput Function Determination

Gavin MacBeath,1* Stuart L. Schreiber2

Systematic efforts are currently under way to construct defined sets of cloned genes for high-throughput expression and purification of recombinant proteins. To facilitate subsequent studies of protein function, we have developed miniaturized assays that accommodate extremely low sample volumes and enable the rapid, simultaneous processing of thousands of proteins. A high-precision robot designed to manufacture complementary DNA microarrays was used to spot proteins onto chemically derivatized glass slides at extremely high spatial densities. The proteins attached covalently to the slide surface yet retained their ability to interact specifically with other proteins, or with small molecules, in solution. Three applications for protein microarrays were demonstrated: screening for protein-protein interactions, identifying the substrates of protein kinases, and identifying the protein targets of small molecules.

1 Center for Genomics Research, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
2 Howard Hughes Medical Institute (HHMI), Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
*   To whom correspondence should be addressed. E-mail: gavin_macbeath{at}harvard.edu

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