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Science 14 July 2000:
Vol. 289. no. 5477, pp. 265 - 270
DOI: 10.1126/science.289.5477.265
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Research Articles

Role of the Mouse ank Gene in Control of Tissue Calcification and Arthritis

Andrew M. Ho, 1* Michelle D. Johnson, 1* David M. Kingsley 12dagger

Mutation at the mouse progressive ankylosis (ank) locus causes a generalized, progressive form of arthritis accompanied by mineral deposition, formation of bony outgrowths, and joint destruction. Here, we show that the ank locus encodes a multipass transmembrane protein (ANK) that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. A highly conserved gene is present in humans and other vertebrates. These results identify ANK-mediated control of pyrophosphate levels as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.

1 Department of Developmental Biology and
2 Howard Hughes Medical Institute, Beckman Center B300, Stanford University School of Medicine, Stanford, CA 94305-5327, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: kingsley{at}cmgm.stanford.edu

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