Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 30 June 2000:
Vol. 288. no. 5475, pp. 2360 - 2363
DOI: 10.1126/science.288.5475.2360
Prev | Table of Contents | Next

Reports

Perception of Brassinosteroids by the Extracellular Domain of the Receptor Kinase BRI1

Zuhua He, 134 Zhi-Yong Wang, 1 Jianming Li, 1* Qun Zhu, 1dagger Chris Lamb, 1ddagger Pamela Ronald, 3 Joanne Chory 12§

An assay was developed to study plant receptor kinase activation and signaling mechanisms. The extracellular leucine-rich repeat (LRR) and transmembrane domains of the Arabidopsis receptor kinase BRI1, which is implicated in brassinosteroid signaling, were fused to the serine/threonine kinase domain of XA21, the rice disease resistance receptor. The chimeric receptor initiates plant defense responses in rice cells upon treatment with brassinosteroids. These results, which indicate that the extracellular domain of BRI1 perceives brassinosteroids, suggest a general signaling mechanism for the LRR receptor kinases of plants. This system should allow the discovery of ligands for the LRR kinases, the largest group of plant receptor kinases.

1 Plant Biology Laboratory,
2 The Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
3 Department of Plant Pathology, University of California, Davis, CA 95616, USA.
4 Biotechnology Institute, Zhejiang University, Hangzhou 310029, China.
*   Present address: Department of Biology, University of Michigan, MI 48190-1048, USA.

dagger    Present address: Dupont Company, Wilmington, DE 19880-0402, USA.

ddagger    Present address: John Innes Centre, Norwich, NR4 7UH, UK.

§   To whom correspondence should be addressed. E-mail: chory{at}salk.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Receptor-mediated signalling in plants: molecular patterns and programmes.
M. Tor, M. T. Lotze, and N. Holton (2009)
J. Exp. Bot. 60, 3645-3654
   Abstract »    Full Text »    PDF »
The primary signaling outputs of brassinosteroids are regulated by abscisic acid signaling.
S. Zhang, Z. Cai, and X. Wang (2009)
PNAS 106, 4543-4548
   Abstract »    Full Text »    PDF »
Cotton metallothionein GhMT3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast.
T. Xue, X. Li, W. Zhu, C. Wu, G. Yang, and C. Zheng (2009)
J. Exp. Bot. 60, 339-349
   Abstract »    Full Text »    PDF »
The Receptor-Like Cytoplasmic Kinase (OsRLCK) Gene Family in Rice: Organization, Phylogenetic Relationship, and Expression during Development and Stress.
S. Vij, J. Giri, P. K. Dansana, S. Kapoor, and A. K. Tyagi (2008)
Mol Plant 1, 732-750
   Abstract »    Full Text »    PDF »
From the Cover: Modulation of brassinosteroid-regulated gene expression by jumonji domain-containing proteins ELF6 and REF6 in Arabidopsis.
X. Yu, L. Li, L. Li, M. Guo, J. Chory, and Y. Yin (2008)
PNAS 105, 7618-7623
   Abstract »    Full Text »    PDF »
Brassinosteroid transport.
G. M. Symons, J. J. Ross, C. E. Jager, and J. B. Reid (2008)
J. Exp. Bot. 59, 17-24
   Abstract »    Full Text »    PDF »
Nucleocytoplasmic Shuttling of BZR1 Mediated by Phosphorylation Is Essential in Arabidopsis Brassinosteroid Signaling.
H. Ryu, K. Kim, H. Cho, J. Park, S. Choe, and I. Hwang (2007)
PLANT CELL 19, 2749-2762
   Abstract »    Full Text »    PDF »
Attenuation of brassinosteroid signaling enhances FLC expression and delays flowering.
M. A. Domagalska, F. M. Schomburg, R. M. Amasino, R. D. Vierstra, F. Nagy, and S. J. Davis (2007)
Development 134, 2841-2850
   Abstract »    Full Text »    PDF »
Brassinosteroid Signaling Pathway.
Y. Belkhadir, X. Wang, and J. Chory (2006)
Sci. STKE 2006, cm4
   Abstract »    Full Text »    PDF »
The BAM1/BAM2 Receptor-Like Kinases Are Important Regulators of Arabidopsis Early Anther Development.
C. L.H. Hord, C. Chen, B. J. DeYoung, S. E. Clark, and H. Ma (2006)
PLANT CELL 18, 1667-1680
   Abstract »    Full Text »    PDF »
Morphological Alteration Caused by Brassinosteroid Insensitivity Increases the Biomass and Grain Production of Rice.
Y. Morinaka, T. Sakamoto, Y. Inukai, M. Agetsuma, H. Kitano, M. Ashikari, and M. Matsuoka (2006)
Plant Physiology 141, 924-931
   Abstract »    Full Text »    PDF »
The Rice 14-3-3 Gene Family and its Involvement in Responses to Biotic and Abiotic Stress.
F. Chen, Q. Li, L. Sun, and Z. He (2006)
DNA Res 13, 53-63
   Abstract »    Full Text »    PDF »
A novel gene family in Arabidopsis encoding putative heptahelical transmembrane proteins homologous to human adiponectin receptors and progestin receptors.
M.-H. Hsieh and H. M. Goodman (2005)
J. Exp. Bot. 56, 3137-3147
   Abstract »    Full Text »    PDF »
A Brassinosteroid-Hypersensitive Mutant of BAK1 Indicates That a Convergence of Photomorphogenic and Hormonal Signaling Modulates Phototropism.
C. W. Whippo and R. P. Hangarter (2005)
Plant Physiology 139, 448-457
   Abstract »    Full Text »    PDF »
Leucine-Rich Repeat Receptor-Like Kinase1 Is a Key Membrane-Bound Regulator of Abscisic Acid Early Signaling in Arabidopsis.
Y. Osakabe, K. Maruyama, M. Seki, M. Satou, K. Shinozaki, and K. Yamaguchi-Shinozaki (2005)
PLANT CELL 17, 1105-1119
   Abstract »    Full Text »    PDF »
Wall-associated Kinase WAK1 Interacts with Cell Wall Pectins in a Calcium-induced Conformation.
A. Decreux and J. Messiaen (2005)
Plant Cell Physiol. 46, 268-278
   Abstract »    Full Text »    PDF »
Heterodimerization and Endocytosis of Arabidopsis Brassinosteroid Receptors BRI1 and AtSERK3 (BAK1).
E. Russinova, J.-W. Borst, M. Kwaaitaal, A. Cano-Delgado, Y. Yin, J. Chory, and S. C. de Vries (2004)
PLANT CELL 16, 3216-3229
   Abstract »    Full Text »    PDF »
BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis.
A. Cano-Delgado, Y. Yin, C. Yu, D. Vafeados, S. Mora-Garcia, J.-C. Cheng, K. H. Nam, J. Li, and J. Chory (2004)
Development 131, 5341-5351
   Abstract »    Full Text »    PDF »
The Arabidopsis Transthyretin-Like Protein Is a Potential Substrate of BRASSINOSTEROID-INSENSITIVE 1.
K. H. Nam and J. Li (2004)
PLANT CELL 16, 2406-2417
   Abstract »    Full Text »    PDF »
Down-Regulation of Metallothionein, a Reactive Oxygen Scavenger, by the Small GTPase OsRac1 in Rice.
H. L. Wong, T. Sakamoto, T. Kawasaki, K. Umemura, and K. Shimamoto (2004)
Plant Physiology 135, 1447-1456
   Abstract »    Full Text »    PDF »
Comparative Analysis of the Receptor-Like Kinase Family in Arabidopsis and Rice.
S.-H. Shiu, W. M. Karlowski, R. Pan, Y.-H. Tzeng, K. F. X. Mayer, and W.-H. Li (2004)
PLANT CELL 16, 1220-1234
   Abstract »    Full Text »    PDF »
Plant Hormone Binding Sites.
R. NAPIER (2004)
Ann. Bot. 93, 227-233
   Abstract »    Full Text »    PDF »
LRR-containing receptors regulating plant development and defense.
A. Dievart and S. E. Clark (2004)
Development 131, 251-261
   Abstract »    Full Text »    PDF »
BRing it on: new insights into the mechanism of brassinosteroid action.
J. L. Nemhauser and J. Chory (2004)
J. Exp. Bot. 55, 265-270
   Abstract »    Full Text »    PDF »
The ULTRACURVATA2 Gene of Arabidopsis Encodes an FK506-Binding Protein Involved in Auxin and Brassinosteroid Signaling.
J. M. Perez-Perez, M. R. Ponce, and J. L. Micol (2004)
Plant Physiology 134, 101-117
   Abstract »    Full Text »    PDF »
A Semidwarf Phenotype of Barley uzu Results from a Nucleotide Substitution in the Gene Encoding a Putative Brassinosteroid Receptor.
M. Chono, I. Honda, H. Zeniya, K. Yoneyama, D. Saisho, K. Takeda, S. Takatsuto, T. Hoshino, and Y. Watanabe (2003)
Plant Physiology 133, 1209-1219
   Abstract »    Full Text »    PDF »
Ligand-receptor pairs in plant peptide signaling.
Y. Matsubayashi (2003)
J. Cell Sci. 116, 3863-3870
   Abstract »    Full Text »    PDF »
CLAVATA1 Dominant-Negative Alleles Reveal Functional Overlap between Multiple Receptor Kinases That Regulate Meristem and Organ Development.
A. Dievart, M. Dalal, F. E. Tax, A. D. Lacey, A. Huttly, J. Li, and S. E. Clark (2003)
PLANT CELL 15, 1198-1211
   Abstract »    Full Text »
Steroid signaling in plants and insects---common themes, different pathways.
C. S. Thummel and J. Chory (2002)
Genes & Dev. 16, 3113-3129
   Full Text »    PDF »
Cloning the Tomato Curl3 Gene Highlights the Putative Dual Role of the Leucine-Rich Repeat Receptor Kinase tBRI1/SR160 in Plant Steroid Hormone and Peptide Hormone Signaling.
T. Montoya, T. Nomura, K. Farrar, T. Kaneta, T. Yokota, and G. J. Bishop (2002)
PLANT CELL 14, 3163-3176
   Abstract »    Full Text »    PDF »
Arabidopsis Brassinosteroid-Insensitive dwarf12 Mutants Are Semidominant and Defective in a Glycogen Synthase Kinase 3beta -Like Kinase.
S. Choe, R. J. Schmitz, S. Fujioka, S. Takatsuto, M.-O. Lee, S. Yoshida, K. A. Feldmann, and F. E. Tax (2002)
Plant Physiology 130, 1506-1515
   Abstract »    Full Text »    PDF »
VH1, a Provascular Cell-Specific Receptor Kinase That Influences Leaf Cell Patterns in Arabidopsis.
N. K. Clay and T. Nelson (2002)
PLANT CELL 14, 2707-2722
   Abstract »    Full Text »    PDF »
Two Putative BIN2 Substrates Are Nuclear Components of Brassinosteroid Signaling.
J. Zhao, P. Peng, R. J. Schmitz, A. D. Decker, F. E. Tax, and J. Li (2002)
Plant Physiology 130, 1221-1229
   Abstract »    Full Text »    PDF »
The heterotrimeric G protein alpha subunit acts upstream of the small GTPase Rac in disease resistance of rice.
U. Suharsono, Y. Fujisawa, T. Kawasaki, Y. Iwasaki, H. Satoh, and K. Shimamoto (2002)
PNAS 99, 13307-13312
   Abstract »    Full Text »    PDF »
Transcriptional and Posttranscriptional Regulation of Arabidopsis TCH4 Expression by Diverse Stimuli. Roles of cis Regions and Brassinosteroids.
E. A. Iliev, W. Xu, D. H. Polisensky, M.-H. Oh, R. S. Torisky, S. D. Clouse, and J. Braam (2002)
Plant Physiology 130, 770-783
   Abstract »    Full Text »    PDF »
A Cysteine-Rich Extracellular Protein, LAT52, Interacts with the Extracellular Domain of the Pollen Receptor Kinase LePRK2.
W. Tang, I. Ezcurra, J. Muschietti, and S. McCormick (2002)
PLANT CELL 14, 2277-2287
   Abstract »    Full Text »    PDF »
Nongenomic Testosterone Calcium Signaling. GENOTROPIC ACTIONS IN ANDROGEN RECEPTOR-FREE MACROPHAGES.
Z. Guo, W. P. M. Benten, J. Krucken, and F. Wunderlich (2002)
J. Biol. Chem. 277, 29600-29607
   Abstract »    Full Text »    PDF »
The EXCESS MICROSPOROCYTES1 gene encodes a putative leucine-rich repeat receptor protein kinase that controls somatic and reproductive cell fates in the Arabidopsis anther.
D.-Z. Zhao, G.-F. Wang, B. Speal, and H. Ma (2002)
Genes & Dev. 16, 2021-2031
   Abstract »    Full Text »    PDF »
A crucial role for the putative Arabidopsis topoisomerase VI in plant growth and development.
Y. Yin, H. Cheong, D. Friedrichsen, Y. Zhao, J. Hu, S. Mora-Garcia, and J. Chory (2002)
PNAS 99, 10191-10196
   Abstract »    Full Text »    PDF »
Plant receptor kinases: Systemin receptor identified.
Y. Yin, D. Wu, and J. Chory (2002)
PNAS 99, 9090-9092
   Full Text »    PDF »
From the Cover: The systemin receptor SR160 from Lycopersicon peruvianum is a member of the LRR receptor kinase family.
J. M. Scheer and C. A. Ryan Jr. (2002)
PNAS 99, 9585-9590
   Abstract »    Full Text »    PDF »
Role of a Heterotrimeric G Protein in Regulation of Arabidopsis Seed Germination.
H. Ullah, J.-G. Chen, S. Wang, and A. M. Jones (2002)
Plant Physiology 129, 897-907
   Abstract »    Full Text »    PDF »
Brassinosteroids and Plant Steroid Hormone Signaling.
G. J. Bishop and C. Koncz (2002)
PLANT CELL 14, S97-110
   Full Text »    PDF »
Self-Incompatibility in the Brassicaceae: Receptor-Ligand Signaling and Cell-to-Cell Communication.
A. Kachroo, M. E. Nasrallah, and J. B. Nasrallah (2002)
PLANT CELL 14, S227-238
   Full Text »    PDF »
Regulation of Brassinosteroid Signaling by a GSK3/SHAGGY-Like Kinase.
J. Li and K. H. Nam (2002)
Science 295, 1299-1301
   Abstract »    Full Text »    PDF »
Plant Receptor-Like Kinase Gene Family: Diversity, Function, and Signaling.
S.-H. Shiu and A. B. Bleecker (2001)
Sci. STKE 2001, re22
   Abstract »    Full Text »    PDF »
Sentinels of Disease. Plant Resistance Genes.
R. Fluhr (2001)
Plant Physiology 127, 1367-1374
   Full Text »    PDF »
Sensitivity of Different Ecotypes and Mutants of Arabidopsis thaliana toward the Bacterial Elicitor Flagellin Correlates with the Presence of Receptor-binding Sites.
Z. Bauer, L. Gomez-Gomez, T. Boller, and G. Felix (2001)
J. Biol. Chem. 276, 45669-45676
   Abstract »    Full Text »    PDF »
The Arabidopsis Somatic Embryogenesis Receptor Kinase 1 Gene Is Expressed in Developing Ovules and Embryos and Enhances Embryogenic Competence in Culture.
V. Hecht, J.-P. Vielle-Calzada, M. V. Hartog, E. D.L. Schmidt, K. Boutilier, U. Grossniklaus, and S. C. de Vries (2001)
Plant Physiology 127, 803-816
   Abstract »    Full Text »    PDF »
The fasciated ear2 gene encodes a leucine-rich repeat receptor-like protein that regulates shoot meristem proliferation in maize.
F. Taguchi-Shiobara, Z. Yuan, S. Hake, and D. Jackson (2001)
Genes & Dev. 15, 2755-2766
   Abstract »    Full Text »    PDF »
Control of Specific Gene Expression by Gibberellin and Brassinosteroid.
T. Bouquin, C. Meier, R. Foster, M. E. Nielsen, and J. Mundy (2001)
Plant Physiology 127, 450-458
   Abstract »    Full Text »    PDF »
BIN2, a New Brassinosteroid-Insensitive Locus in Arabidopsis.
J. Li, K. H. Nam, D. Vafeados, and J. Chory (2001)
Plant Physiology 127, 14-22
   Abstract »    Full Text »    PDF »
Isolation and Characterization of Kinase Interacting Protein 1, a Pollen Protein That Interacts with the Kinase Domain of PRK1, a Receptor-Like Kinase of Petunia.
A. L. Skirpan, A. G. McCubbin, T. Ishimizu, X. Wang, Y. Hu, P. E. Dowd, H. Ma, and T.-h. Kao (2001)
Plant Physiology 126, 1480-1492
   Abstract »    Full Text »    PDF »
A Large Family of Genes That Share Homology with CLAVATA3.
J. M. Cock and S. McCormick (2001)
Plant Physiology 126, 939-942
   Full Text »    PDF »
A Superfamily of Proteins with Novel Cysteine-Rich Repeats.
Z. Chen (2001)
Plant Physiology 126, 473-476
   Full Text »    PDF »
BRS1, a serine carboxypeptidase, regulates BRI1 signaling in Arabidopsis thaliana.
J. Li, K. A. Lease, F. E. Tax, and J. C. Walker (2001)
PNAS
   Abstract »    Full Text »
Plants Steroid Hormones, Brassinosteroids: Current Highlights of Molecular Aspects on their Synthesis/Metabolism, Transport, Perception and Response.
G. J. Bishop and T. Yokota (2001)
Plant Cell Physiol. 42, 114-120
   Abstract »    Full Text »    PDF »
Brassinosteroid Levels Increase Drastically Prior to Morphogenesis of Tracheary Elements.
R. Yamamoto, S. Fujioka, T. Demura, S. Takatsuto, S. Yoshida, and H. Fukuda (2001)
Plant Physiology 125, 556-563
   Abstract »    Full Text »
A Role for Brassinosteroids in Germination in Arabidopsis.
C. M. Steber and P. McCourt (2001)
Plant Physiology 125, 763-769
   Abstract »    Full Text »
Hormone Response Mutants. A Plethora of Surprises.
H. Kende (2001)
Plant Physiology 125, 81-84
   Full Text »
Interaction of the Arabidopsis Receptor Protein Kinase Wak1 with a Glycine-rich Protein, AtGRP-3.
A. R. Park, S. K. Cho, U. J. Yun, M. Y. Jin, S. H. Lee, G. Sachetto-Martins, and O. K. Park (2001)
J. Biol. Chem. 276, 26688-26693
   Abstract »    Full Text »    PDF »
Essential role of the small GTPase Rac in disease resistance of rice.
E. Ono, H. L. Wong, T. Kawasaki, M. Hasegawa, O. Kodama, and K. Shimamoto (2001)
PNAS 98, 759-764
   Abstract »    Full Text »    PDF »
BRS1, a serine carboxypeptidase, regulates BRI1 signaling in Arabidopsis thaliana.
J. Li, K. A. Lease, F. E. Tax, and J. C. Walker (2001)
PNAS 98, 5916-5921
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)