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Science 2 June 2000:
Vol. 288. no. 5471, pp. 1643 - 1647
DOI: 10.1126/science.288.5471.1643
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Reports

Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression

Karim Labib, José Antonio Tercero, John F. X. Diffley *

Little is known about the DNA helicases required for the elongation phase of eukaryotic chromosome replication. Minichromosome maintenance (MCM) protein complexes have DNA helicase activity but have only been functionally implicated in initiating DNA replication. Using an improved method for constructing conditional degron mutants, we show that depletion of MCMs after initiation irreversibly blocks the progression of replication forks in Saccharomyces cerevisiae. Like the Escherichia coli dnaB and SV40 T antigen helicases, therefore, the MCM complex is loaded at origins before initiation and is essential for elongation. Restricting MCM loading to the G1 phase ensures that initiation and elongation occur just once per cell cycle.

Imperial Cancer Research Fund Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, UK.
*   To whom correspondence should be addressed. E-mail: J.Diffley{at}icrf.icnet.uk

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Identification of MCM4 as a Target of the DNA Replication Block Checkpoint System.
Y. Ishimi, Y. Komamura-Kohno, H.-J. Kwon, K. Yamada, and M. Nakanishi (2003)
J. Biol. Chem. 278, 24644-24650
   Abstract »    Full Text »    PDF »
Peroxisome Proliferator-Activated Receptor {gamma} Inhibits Expression of Minichromosome Maintenance Proteins in Vascular Smooth Muscle Cells.
D. Bruemmer, F. Yin, J. Liu, J. P. Berger, T. Kiyono, J. Chen, E. Fleck, A. J. Van Herle, B. M. Forman, and R. E. Law (2003)
Mol. Endocrinol. 17, 1005-1018
   Abstract »    Full Text »    PDF »
Physical and Functional Interaction of the Yeast Corepressor Tup1 with mRNA 5'-Triphosphatase.
Y. Mukai, J. K. Davie, and S. Y. R. Dent (2003)
J. Biol. Chem. 278, 18895-18901
   Abstract »    Full Text »    PDF »
A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication.
Y. Kubota, Y. Takase, Y. Komori, Y. Hashimoto, T. Arata, Y. Kamimura, H. Araki, and H. Takisawa (2003)
Genes & Dev. 17, 1141-1152
   Abstract »    Full Text »    PDF »
GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast.
Y. Takayama, Y. Kamimura, M. Okawa, S. Muramatsu, A. Sugino, and H. Araki (2003)
Genes & Dev. 17, 1153-1165
   Abstract »    Full Text »    PDF »


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