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Science 2 June 2000:
Vol. 288. no. 5471, pp. 1640 - 1643
DOI: 10.1126/science.288.5471.1640
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Reports

Role of 4.5S RNA in Assembly of the Bacterial Signal Recognition Particle with Its Receptor

Paul Peluso, 1 Daniel Herschlag, 2 Silke Nock, 1 Douglas M. Freymann, * Arthur E. Johnson, 3 Peter Walter 1dagger

The mechanism by which a signal recognition particle (SRP) and its receptor mediate protein targeting to the endoplasmic reticulum or to the bacterial plasma membrane is evolutionarily conserved. In Escherichia coli, this reaction is mediated by the Ffh/4.5S RNA ribonucleoprotein complex (Ffh/4.5S RNP; the SRP) and the FtsY protein (the SRP receptor). We have quantified the effects of 4.5S RNA on Ffh-FtsY complex formation by monitoring changes in tryptophan fluorescence. Surprisingly, 4.5S RNA facilitates both assembly and disassembly of the Ffh-FtsY complex to a similar extent. These results provide an example of an RNA molecule facilitating protein-protein interactions in a catalytic fashion.

1 Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA.
2 Department of Biochemistry, Stanford University, Stanford, CA 94305, USA.
3 System Health Science Center, Texas A&M University, College Station, TX 77843, USA.
*   Present address: Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611, USA.

dagger    To whom correspondence should be addressed. E-mail: walter{at}cgl.ucsf.edu

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