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Science 24 March 2000:
Vol. 287. no. 5461, pp. 2271 - 2274
DOI: 10.1126/science.287.5461.2271
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Reports

A BAC-Based Physical Map of the Major Autosomes of Drosophila melanogaster

Roger A. Hoskins, 1* Catherine R. Nelson, 2 Benjamin P. Berman, 2 Todd R. Laverty, 2 Reed A. George, 1 Lisa Ciesiolka, 1 Mohammed Naeemuddin, 1 Andrew D. Arenson, 3 James Durbin, 3 Robert G. David, 3 Paul E. Tabor, 3 Michael R. Bailey, 3 Denise R. DeShazo, 3 Joseph Catanese, 4 †‡ Aaron Mammoser, 4 Kazutoyo Osoegawa, 4 † Pieter J. de Jong , 4 †‡ Susan E. Celniker, 1 Richard A. Gibbs, 3 Gerald M. Rubin, 2 Steven E. Scherer 3

We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.

1 Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
2 Berkeley Drosophila Genome Project, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
3 Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX 77030, USA.
4 Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
*   To whom correspondence should be addressed. E-mail: hoskins{at}bdgp.lbl.gov

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Science. ISSN 0036-8075 (print), 1095-9203 (online)