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Science 24 March 2000:
Vol. 287. no. 5461, pp. 2196 - 2204
DOI: 10.1126/science.287.5461.2196
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Review

A Whole-Genome Assembly of Drosophila

Eugene W. Myers, 1* Granger G. Sutton, 1 Art L. Delcher, 1 Ian M. Dew, 1 Dan P. Fasulo, 1 Michael J. Flanigan, 1 Saul A. Kravitz, 1 Clark M. Mobarry, 1 Knut H. J. Reinert, 1 Karin A. Remington, 1 Eric L. Anson, 1 Randall A. Bolanos, 1 Hui-Hsien Chou, 1 Catherine M. Jordan, 1 Aaron L. Halpern, 1 Stefano Lonardi, 1 Ellen M. Beasley, 1 Rhonda C. Brandon, 1 Lin Chen, 1 Patrick J. Dunn, 1 Zhongwu Lai, 1 Yong Liang, 1 Deborah R. Nusskern, 1 Ming Zhan, 1 Qing Zhang, 1 Xiangqun Zheng, 1 Gerald M. Rubin, 2 Mark D. Adams, 1 J. Craig Venter 1

We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9- megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99.99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.

1 Celera Genomics, Inc., 45 West Gude Drive, Rockville, MD 20850, USA.
2 Howard Hughes Medical Institute, Berkeley Drosophila Genome Project, University of California, Berkeley, CA 94720, USA.
*   To whom correspondence should be sent: Gene.Myers{at}celera.com

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