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Science 7 January 2000:
Vol. 287. no. 5450, pp. 131 - 134
DOI: 10.1126/science.287.5450.131
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Reports

DNA Topoisomerase IIbeta and Neural Development

Xia Yang, 1* Wei Li, 2*dagger Elizabeth D. Prescott, 1ddagger Steven J. Burden, 1 James C. Wang 2

DNA topoisomerase IIbeta is shown to have an unsuspected and critical role in neural development. Neurogenesis was normal in IIbeta mutant mice, but motor axons failed to contact skeletal muscles, and sensory axons failed to enter the spinal cord. Despite an absence of innervation, clusters of acetylcholine receptors were concentrated in the central region of skeletal muscles, thereby revealing patterning mechanisms that are autonomous to skeletal muscle. The defects in motor axon growth in IIbeta mutant mice resulted in a breathing impairment and death of the pups shortly after birth.

1 Skirball Institute of Molecular Medicine, New York University Medical School, 540 First Avenue, New York, NY 10016, USA.
2 Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA.
*   These authors contributed equally to this work.

dagger    Present address: Vertex Pharmaceuticals, 40 Allison Street, Cambridge, MA 02139, USA.

ddagger    Present address: Department of Biochemistry and Biophysics, University of California at San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA.

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