Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 7 January 2000:
Vol. 287. no. 5450, pp. 116 - 122
DOI: 10.1126/science.287.5450.116
Prev | Table of Contents | Next

Reports

Protein Interaction Mapping in C. elegans Using Proteins Involved in Vulval Development

Albertha J. M. Walhout, 1* Raffaella Sordella, 1 Xiaowei Lu, 2dagger James L. Hartley, 3 Gary F. Temple, 3 Michael A. Brasch, 3 Nicolas Thierry-Mieg, 14 Marc Vidal 1*ddagger

Protein interaction mapping using large-scale two-hybrid analysis has been proposed as a way to functionally annotate large numbers of uncharacterized proteins predicted by complete genome sequences. This approach was examined in Caenorhabditis elegans, starting with 27 proteins involved in vulval development. The resulting map reveals both known and new potential interactions and provides a functional annotation for approximately 100 uncharacterized gene products. A protein interaction mapping project is now feasible for C. elegans on a genome-wide scale and should contribute to the understanding of molecular mechanisms in this organism and in human diseases.

1 Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA.
2 Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
3 Life Technologies Inc., Rockville, MD 20850, USA.
4 Laboratoire LSR-IMAG, 38402 St-Martin-D'Heres Cedex, France.
*   Present address: Dana Farber Cancer Institute, Department of Genetics, Harvard Medical School, Boston, MA 02115, U.S.A.

dagger    Present address: Department of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA.

ddagger    To whom correspondence should be addressed. E-mail: Marc_Vidal{at}DFCI.Harvard.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Gene-centered regulatory networks.
H. E. Arda and A. J.M. Walhout (2009)
Brief Funct Genomic Proteomic
   Abstract »    Full Text »    PDF »
Large-scale RACE approach for proactive experimental definition of C. elegans ORFeome.
K. Salehi-Ashtiani, C. Lin, T. Hao, Y. Shen, D. Szeto, X. Yang, L. Ghamsari, H. Lee, C. Fan, R. R. Murray, et al. (2009)
Genome Res. 19, 2334-2342
   Abstract »    Full Text »    PDF »
Simultaneous inference of biological networks of multiple species from genome-wide data and evolutionary information: a semi-supervised approach.
H. Kashima, Y. Yamanishi, T. Kato, M. Sugiyama, and K. Tsuda (2009)
Bioinformatics 25, 2962-2968
   Abstract »    Full Text »    PDF »
The human protein coevolution network.
E. R.M. Tillier and R. L. Charlebois (2009)
Genome Res. 19, 1861-1871
   Abstract »    Full Text »    PDF »
Polo-like Kinase 1 (PLK1) Regulates Interferon (IFN) Induction by MAVS.
D. Vitour, S. Dabo, M. Ahmadi Pour, M. Vilasco, P.-O. Vidalain, Y. Jacob, M. Mezel-Lemoine, S. Paz, M. Arguello, R. Lin, et al. (2009)
J. Biol. Chem. 284, 21797-21809
   Abstract »    Full Text »    PDF »
Marian Walhout: Transcriptional mapmaker.
B. Short (2009)
J. Cell Biol. 186, 4-5
   Full Text »    PDF »
The Cartographers toolbox: building bigger and better human protein interaction networks.
C. M. Sanderson (2009)
Brief Funct Genomic Proteomic
   Abstract »    Full Text »    PDF »
High-Quality Binary Protein Interaction Map of the Yeast Interactome Network.
H. Yu, P. Braun, M. A. Yildirim, I. Lemmens, K. Venkatesan, J. Sahalie, T. Hirozane-Kishikawa, F. Gebreab, N. Li, N. Simonis, et al. (2008)
Science 322, 104-110
   Abstract »    Full Text »    PDF »
Division protein interaction web: identification of a phylogenetically conserved common interactome between Streptococcus pneumoniae and Escherichia coli.
S. Maggi, O. Massidda, G. Luzi, D. Fadda, L. Paolozzi, and P. Ghelardini (2008)
Microbiology 154, 3042-3052
   Abstract »    Full Text »    PDF »
A C. elegans genome-scale microRNA network contains composite feedback motifs with high flux capacity.
N. J. Martinez, M. C. Ow, M. I. Barrasa, M. Hammell, R. Sequerra, L. Doucette-Stamm, F. P. Roth, V. R. Ambros, and A. J.M. Walhout (2008)
Genes & Dev. 22, 2535-2549
   Abstract »    Full Text »    PDF »
InteroPORC: automated inference of highly conserved protein interaction networks.
M. Michaut, S. Kerrien, L. Montecchi-Palazzi, F. Chauvat, C. Cassier-Chauvat, J.-C. Aude, P. Legrain, and H. Hermjakob (2008)
Bioinformatics 24, 1625-1631
   Abstract »    Full Text »    PDF »
Genetic networks for the functional study of genomes.
A. G. Pisabarro, G. Perez, J. L. Lavin, and L. Ramirez (2008)
Brief Funct Genomic Proteomic 7, 249-263
   Abstract »    Full Text »    PDF »
MassNet: a functional annotation service for protein mass spectrometry data.
D. Park, B.-C. Kim, S.-W. Cho, S.-J. Park, J.-S. Choi, S. I. Kim, J. Bhak, and S. Lee (2008)
Nucleic Acids Res. 36, W491-W495
   Abstract »    Full Text »    PDF »
Network-guided genetic screening: building, testing and using gene networks to predict gene function.
B. Lehner and I. Lee (2008)
Brief Funct Genomic Proteomic 7, 217-227
   Abstract »    Full Text »    PDF »
A new way to explore the world of extracellular protein interactions.
C. M. Sanderson (2008)
Genome Res. 18, 517-520
   Abstract »    Full Text »    PDF »
An assessment of the uses of homologous interactions.
R. Saeed and C. Deane (2008)
Bioinformatics 24, 689-695
   Abstract »    Full Text »    PDF »
A Large Scale Analysis of Protein-Protein Interactions in the Nitrogen-fixing Bacterium Mesorhizobium loti.
Y. Shimoda, S. Shinpo, M. Kohara, Y. Nakamura, S. Tabata, and S. Sato (2008)
DNA Res 15, 13-23
   Abstract »    Full Text »    PDF »
Synthetic Lethal Interactions Identify Phenotypic "Interologs" of the Spindle Assembly Checkpoint Components.
M. Tarailo, S. Tarailo, and A. M. Rose (2007)
Genetics 177, 2525-2530
   Abstract »    Full Text »    PDF »
Recombinational Cloning with Plant Gateway Vectors.
M. Karimi, A. Depicker, and P. Hilson (2007)
Plant Physiology 145, 1144-1154
   Full Text »    PDF »
A Large-scale Protein protein Interaction Analysis in Synechocystis sp. PCC6803.
S. Sato, Y. Shimoda, A. Muraki, M. Kohara, Y. Nakamura, and S. Tabata (2007)
DNA Res
   Abstract »    Full Text »    PDF »
Transcription factor modularity in a gene-centered C. elegans core neuronal protein-DNA interaction network.
V. Vermeirssen, M. I. Barrasa, C. A. Hidalgo, J. A. B. Babon, R. Sequerra, L. Doucette-Stamm, A.-L. Barabasi, and A. J.M. Walhout (2007)
Genome Res. 17, 1061-1071
   Abstract »    Full Text »    PDF »
Getting connected: analysis and principles of biological networks.
X. Zhu, M. Gerstein, and M. Snyder (2007)
Genes & Dev. 21, 1010-1024
   Abstract »    Full Text »    PDF »
Epstein-Barr virus and virus human protein interaction maps.
M. A. Calderwood, K. Venkatesan, L. Xing, M. R. Chase, A. Vazquez, A. M. Holthaus, A. E. Ewence, N. Li, T. Hirozane-Kishikawa, D. E. Hill, et al. (2007)
PNAS 104, 7606-7611
   Abstract »    Full Text »    PDF »
Apolipoprotein Receptor 2 and X11{alpha}/{beta} Mediate Apolipoprotein E-Induced Endocytosis of Amyloid-{beta} Precursor Protein and {beta}-Secretase, Leading to Amyloid-{beta} Production.
X. He, K. Cooley, C. H. Y. Chung, N. Dashti, and J. Tang (2007)
J. Neurosci. 27, 4052-4060
   Abstract »    Full Text »    PDF »
DPL-1 DP, LIN-35 Rb and EFL-1 E2F Act With the MCD-1 Zinc-Finger Protein to Promote Programmed Cell Death in Caenorhabditis elegans.
P. W. Reddien, E. C. Andersen, M. C. Huang, and H. R. Horvitz (2007)
Genetics 175, 1719-1733
   Abstract »    Full Text »    PDF »
Human Papillomavirus Type 5 E6 Oncoprotein Represses the Transforming Growth Factor {beta} Signaling Pathway by Binding to SMAD3.
J.-A. Mendoza, Y. Jacob, P. Cassonnet, and M. Favre (2006)
J. Virol. 80, 12420-12424
   Abstract »    Full Text »    PDF »
Unraveling transcription regulatory networks by protein-DNA and protein-protein interaction mapping.
A. J.M. Walhout (2006)
Genome Res. 16, 1445-1454
   Abstract »    Full Text »    PDF »
Some C. elegans class B synthetic multivulva proteins encode a conserved LIN-35 Rb-containing complex distinct from a NuRD-like complex.
M. M. Harrison, C. J. Ceol, X. Lu, and H. R. Horvitz (2006)
PNAS 103, 16782-16787
   Abstract »    Full Text »    PDF »
Gateway-Compatible Yeast One-Hybrid Screens.
B. Deplancke, V. Vermeirssen, H. E. Arda, N. J. Martinez, and A. J.M. Walhout (2006)
CSH Protocols 2006, pdb.prot4590
   Abstract »    Full Text »
Endocrine signaling in Caenorhabditis elegans controls stress response and longevity..
R. Baumeister, E. Schaffitzel, and M. Hertweck (2006)
J. Endocrinol. 190, 191-202
   Abstract »    Full Text »    PDF »
From genome to proteome: developing expression clone resources for the human genome..
G. Temple, P. Lamesch, S. Milstein, D. E. Hill, L. Wagner, T. Moore, and M. Vidal (2006)
Hum. Mol. Genet. 15, R31-R43
   Abstract »    Full Text »    PDF »
The Genetic Architecture of Sucrose Responsiveness in the Honeybee (Apis mellifera L.).
O. Rueppell, S. B. C. Chandra, T. Pankiw, M. K. Fondrk, M. Beye, G. Hunt, and R. E. Page (2006)
Genetics 172, 243-251
   Abstract »    Full Text »    PDF »
lin-8, Which Antagonizes Caenorhabditis elegans Ras-Mediated Vulval Induction, Encodes a Novel Nuclear Protein That Interacts With the LIN-35 Rb Protein.
E. M. Davison, M. M. Harrison, A. J. M. Walhout, M. Vidal, and H. R. Horvitz (2005)
Genetics 171, 1017-1031
   Abstract »    Full Text »    PDF »
Interactome: gateway into systems biology.
M. E. Cusick, N. Klitgord, M. Vidal, and D. E. Hill (2005)
Hum. Mol. Genet. 14, R171-R181
   Abstract »    Full Text »    PDF »
Inferring protein-protein interactions through high-throughput interaction data from diverse organisms.
Y. Liu, N. Liu, and H. Zhao (2005)
Bioinformatics 21, 3279-3285
   Abstract »    Full Text »    PDF »
Functional annotation from predicted protein interaction networks.
J. McDermott, R. Bumgarner, and R. Samudrala (2005)
Bioinformatics 21, 3217-3226
   Abstract »    Full Text »    PDF »
Metabolic Engineering in the -omics Era: Elucidating and Modulating Regulatory Networks.
G. N. Vemuri and A. A. Aristidou (2005)
Microbiol. Mol. Biol. Rev. 69, 197-216
   Abstract »    Full Text »    PDF »
The end of "naive reductionism": rise of systems biology or renaissance of physiology?.
K. Strange (2005)
Am J Physiol Cell Physiol 288, C968-C974
   Abstract »    Full Text »    PDF »
Comprehensive Interaction Map of the Arabidopsis MADS Box Transcription Factors.
S. de Folter, R. G.H. Immink, M. Kieffer, L. Parenicova, S. R. Henz, D. Weigel, M. Busscher, M. Kooiker, L. Colombo, M. M. Kater, et al. (2005)
PLANT CELL 17, 1424-1433
   Abstract »    Full Text »    PDF »
ATP Binding Regulates Oligomerization and Endosome Association of RME-1 Family Proteins.
D.-w. Lee, X. Zhao, S. Scarselletta, P. J. Schweinsberg, E. Eisenberg, B. D. Grant, and L. E. Greene (2005)
J. Biol. Chem. 280, 17213-17220
   Abstract »    Full Text »    PDF »
Experimental and bioinformatic approaches for interrogating protein-protein interactions to determine protein function.
A. Droit, G. G Poirier, and J. M Hunter (2005)
J. Mol. Endocrinol. 34, 263-280
   Abstract »    Full Text »    PDF »
Closing in on the C. elegans ORFeome by cloning TWINSCAN predictions.
C. Wei, P. Lamesch, M. Arumugam, J. Rosenberg, P. Hu, M. Vidal, and M. R. Brent (2005)
Genome Res. 15, 577-582
   Abstract »    Full Text »    PDF »
Protein interaction mapping: A Drosophila case study.
E. Formstecher, S. Aresta, V. Collura, A. Hamburger, A. Meil, A. Trehin, C. Reverdy, V. Betin, S. Maire, C. Brun, et al. (2005)
Genome Res. 15, 376-384
   Abstract »    Full Text »    PDF »
New genes with roles in the C. elegans embryo revealed using RNAi of ovary-enriched ORFeome clones.
A. G. Fernandez, K. C. Gunsalus, J. Huang, L.-S. Chuang, N. Ying, H.-l. Liang, C. Tang, A. J. Schetter, C. Zegar, J.-F. Rual, et al. (2005)
Genome Res. 15, 250-259
   Abstract »    Full Text »    PDF »
RDE-2 interacts with MUT-7 to mediate RNA interference in Caenorhabditis elegans.
B. B. J. Tops, H. Tabara, T. Sijen, F. Simmer, C. C. Mello, R. H. A. Plasterk, and R. F. Ketting (2005)
Nucleic Acids Res. 33, 347-355
   Abstract »    Full Text »    PDF »
Global network analysis of phenotypic effects: Protein networks and toxicity modulation in Saccharomyces cerevisiae.
M. R. Said, T. J. Begley, A. V. Oppenheim, D. A. Lauffenburger, and L. D. Samson (2004)
PNAS 101, 18006-18011
   Abstract »    Full Text »    PDF »
A Lentiviral cDNA Library Employing Lambda Recombination Used To Clone an Inhibitor of Human Immunodeficiency Virus Type 1-Induced Cell Death.
Y. Kawano, T. Yoshida, K. Hieda, J. Aoki, H. Miyoshi, and Y. Koyanagi (2004)
J. Virol. 78, 11352-11359
   Abstract »    Full Text »    PDF »
Genetic Models of Mechanotransduction: The Nematode Caenorhabditis elegans.
P. Syntichaki and N. Tavernarakis (2004)
Physiol Rev 84, 1097-1153
   Abstract »    Full Text »    PDF »
ORFeome Cloning and Systems Biology: Standardized Mass Production of the Parts From the Parts-List.
M. A. Brasch, J. L. Hartley, and M. Vidal (2004)
Genome Res. 14, 2001-2009
   Abstract »    Full Text »    PDF »
Academia-Industry Collaboration: An Integral Element for Building "Omic" Resources.
D. E. Hill, M. A. Brasch, A. A. del Campo, L. Doucette-Stamm, J. I. Garrels, J. Glaven, J. L. Hartley, J. R. Hudson Jr., T. Moore, and M. Vidal (2004)
Genome Res. 14, 2010-2014
   Full Text »    PDF »
C. elegans ORFeome Version 3.1: Increasing the Coverage of ORFeome Resources With Improved Gene Predictions.
P. Lamesch, S. Milstein, T. Hao, J. Rosenberg, N. Li, R. Sequerra, S. Bosak, L. Doucette-Stamm, J. Vandenhaute, D. E. Hill, et al. (2004)
Genome Res. 14, 2064-2069
   Abstract »    Full Text »    PDF »
Feasibility of Genome-Scale Construction of Promoter::Reporter Gene Fusions for Expression in Caenorhabditis elegans Using a MultiSite Gateway Recombination System.
I. A. Hope, J. Stevens, A. Garner, J. Hayes, D. L. Cheo, M. A. Brasch, and M. Vidal (2004)
Genome Res. 14, 2070-2075
   Abstract »    Full Text »    PDF »
A Gateway-Compatible Yeast One-Hybrid System.
B. Deplancke, D. Dupuy, M. Vidal, and A. J.M. Walhout (2004)
Genome Res. 14, 2093-2101
   Abstract »    Full Text »    PDF »
Concerted Assembly and Cloning of Multiple DNA Segments Using In Vitro Site-Specific Recombination: Functional Analysis of Multi-Segment Expression Clones.
D. L. Cheo, S. A. Titus, D. R.N. Byrd, J. L. Hartley, G. F. Temple, and M. A. Brasch (2004)
Genome Res. 14, 2111-2120
   Abstract »    Full Text »    PDF »
Toward Improving Caenorhabditis elegans Phenome Mapping With an ORFeome-Based RNAi Library.
J.-F. Rual, J. Ceron, J. Koreth, T. Hao, A.-S. Nicot, T. Hirozane-Kishikawa, J. Vandenhaute, S. H. Orkin, D. E. Hill, S. van den Heuvel, et al. (2004)
Genome Res. 14, 2162-2168
   Abstract »    Full Text »    PDF »
A First Version of the Caenorhabditis elegans Promoterome.
D. Dupuy, Q.-R. Li, B. Deplancke, M. Boxem, T. Hao, P. Lamesch, R. Sequerra, S. Bosak, L. Doucette-Stamm, I. A. Hope, et al. (2004)
Genome Res. 14, 2169-2175
   Abstract »    Full Text »    PDF »
Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications.
P. Hilson, J. Allemeersch, T. Altmann, S. Aubourg, A. Avon, J. Beynon, R. P. Bhalerao, F. Bitton, M. Caboche, B. Cannoot, et al. (2004)
Genome Res. 14, 2176-2189
   Abstract »    Full Text »    PDF »
Generation of the Brucella melitensis ORFeome Version 1.1.
A. Dricot, J.-F. Rual, P. Lamesch, N. Bertin, D. Dupuy, T. Hao, C. Lambert, R. Hallez, J.-M. Delroisse, J. Vandenhaute, et al. (2004)
Genome Res. 14, 2201-2206
   Abstract »    Full Text »    PDF »
New Protein-Protein Interactions Identified for the Regulatory and Structural Components and Substrates of the Type III Secretion System of the Phytopathogen Xanthomonas axonopodis Pathovar citri.
M. C. Alegria, C. Docena, L. Khater, C. H. I. Ramos, A. C. R. da Silva, and C. S. Farah (2004)
J. Bacteriol. 186, 6186-6197
   Abstract »    Full Text »    PDF »
Antiviral Drug Discovery Strategy Using Combinatorial Libraries of Structurally Constrained Peptides.
E. Real, J.-C. Rain, V. Battaglia, C. Jallet, P. Perrin, N. Tordo, P. Chrisment, J. D'Alayer, P. Legrain, and Y. Jacob (2004)
J. Virol. 78, 7410-7417
   Abstract »    Full Text »    PDF »
A Protein Interaction Framework for Human mRNA Degradation.
B. Lehner and C. M. Sanderson (2004)
Genome Res. 14, 1315-1323
   Abstract »    Full Text »    PDF »
Annotation Transfer Between Genomes: Protein-Protein Interologs and Protein-DNA Regulogs.
H. Yu, N. M. Luscombe, H. X. Lu, X. Zhu, Y. Xia, J.-D. J. Han, N. Bertin, S. Chung, M. Vidal, and M. Gerstein (2004)
Genome Res. 14, 1107-1118
   Abstract »    Full Text »    PDF »
Proteomic research: potential opportunities for clinical and physiological investigators.
K. S. Nair, A. Jaleel, Y. W. Asmann, K. R. Short, and S. Raghavakaimal (2004)
Am J Physiol Endocrinol Metab 286, E863-E874
   Abstract »    Full Text »    PDF »
Identification and Mapping of Self-Assembling Protein Domains Encoded by the Escherichia coli K-12 Genome by Use of {lambda} Repressor Fusions.
L. Marino-Ramirez, J. L. Minor, N. Reading, and J. C. Hu (2004)
J. Bacteriol. 186, 1311-1319
   Abstract »    Full Text »    PDF »
Novel mutations in the KCNQ2 gene link epilepsy to a dysfunction of the KCNQ2-calmodulin interaction.
M C Richards, S E Heron, H E Spendlove, I E Scheffer, B Grinton, S F Berkovic, J C Mulley, and A Davy (2004)
J. Med. Genet. 41, e35-35
   Full Text »    PDF »
Alternative splicing of N- and C-termini of a C. elegans ClC channel alters gating and sensitivity to external Cl- and H+.
J. Denton, K. Nehrke, E. Rutledge, R. Morrison, and K. Strange (2004)
J. Physiol. 555, 97-114
   Abstract »    Full Text »    PDF »
Protein interaction mapping on a functional shotgun sequence of Rickettsia sibirica.
J. A. Malek, J. M. Wierzbowski, W. Tao, S. A. Bosak, D. J. Saranga, L. Doucette-Stamm, D. R. Smith, P. J. McEwan, and K. J. McKernan (2004)
Nucleic Acids Res. 32, 1059-1064
   Abstract »    Full Text »    PDF »
Crosstalk Between the EGFR and LIN-12/Notch Pathways in C. elegans Vulval Development.
A. S. Yoo, C. Bais, and I. Greenwald (2004)
Science 303, 663-666
   Abstract »    Full Text »    PDF »
A Map of the Interactome Network of the Metazoan C. elegans.
S. Li, C. M. Armstrong, N. Bertin, H. Ge, S. Milstein, M. Boxem, P.-O. Vidalain, J.-D. J. Han, A. Chesneau, T. Hao, et al. (2004)
Science 303, 540-543
   Abstract »    Full Text »    PDF »
RNAiDB and PhenoBlast: web tools for genome-wide phenotypic mapping projects.
K. C. Gunsalus, W.-C. Yueh, P. MacMenamin, and F. Piano (2004)
Nucleic Acids Res. 32, D406-410
   Abstract »    Full Text »    PDF »
The Caenorhabditis elegans schnurri homolog sma-9 mediates stage- and cell type-specific responses to DBL-1 BMP-related signaling.
J. Liang, R. Lints, M. L. Foehr, R. Tokarz, L. Yu, S. W. Emmons, J. Liu, and C. Savage-Dunn (2003)
Development 130, 6453-6464
   Abstract »    Full Text »    PDF »
Finding Partners: Emerging Protein Interaction Technologies Applied to Signaling Networks.
I. Stagljar (2003)
Sci. STKE 2003, pe56
   Abstract »    Full Text »    PDF »
A Strategy for Constructing Large Protein Interaction Maps Using the Yeast Two-Hybrid System:Regulated Expression Arrays and Two-Phase Mating.
J. Zhong, H. Zhang, C. A. Stanyon, G. Tromp, and R. L. Finley Jr. (2003)
Genome Res. 13, 2691-2699
   Abstract »    Full Text »    PDF »
The Mammalian Protein-Protein Interaction Database and Its Viewing System That Is Linked to the Main FANTOM2 Viewer.
H. Suzuki, R. Saito, M. Kanamori, C. Kai, C. Schonbach, T. Nagashima, J. Hosaka, and Y. Hayashizaki (2003)
Genome Res. 13, 1534-1541
   Abstract »    Full Text »    PDF »
From Genes to Integrative Physiology: Ion Channel and Transporter Biology in Caenorhabditis elegans.
K. Strange (2003)
Physiol Rev 83, 377-415
   Abstract »    Full Text »    PDF »
A Ral Guanine Exchange Factor-Ral Pathway Is Conserved in Drosophila melanogaster and Sheds New Light on the Connectivity of the Ral, Ras, and Rap Pathways.
G. Mirey, M. Balakireva, S. L'Hoste, C. Rosse, S. Voegeling, and J. Camonis (2003)
Mol. Cell. Biol. 23, 1112-1124
   Abstract »    Full Text »    PDF »
WormBase: a cross-species database for comparative genomics.
T. W. Harris, R. Lee, E. Schwarz, K. Bradnam, D. Lawson, W. Chen, D. Blasier, E. Kenny, F. Cunningham, R. Kishore, et al. (2003)
Nucleic Acids Res. 31, 133-137
   Abstract »    Full Text »    PDF »
WorfDB: the Caenorhabditis elegans ORFeome Database.
P. Vaglio, P. Lamesch, J. Reboul, J.-F. Rual, M. Martinez, D. Hill, and M. Vidal (2003)
Nucleic Acids Res. 31, 237-240
   Abstract »    Full Text »    PDF »
Annotation of Novel Proteins Utilizing A Functional Genome Shotgun Coupled with High-Throughput Protein Interaction Mapping.
J.A. MALEK, J.M. WIERZBOWSKI, G.A. DASCH, M.E. EREMEVA, P.J. MCEWAN, and K.J. MCKERNAN (2003)
Cold Spring Harb Symp Quant Biol 68, 331-334
   Abstract »    PDF »
Microbial Genomics--Challenges and Opportunities: The 9th International Conference on Microbial Genomes.
J. Zhou and J. H. Miller (2002)
J. Bacteriol. 184, 4327-4333
   Full Text »    PDF »
C. elegans EOR-1/PLZF and EOR-2 positively regulate Ras and Wnt signaling and function redundantly with LIN-25 and the SUR-2 Mediator component.
R. M. Howard and M. V. Sundaram (2002)
Genes & Dev. 16, 1815-1827
   Abstract »    Full Text »    PDF »
Central Region of the Human Splicing Factor Hprp3p Interacts with Hprp4p.
J. M. Gonzalez-Santos, A. Wang, J. Jones, C. Ushida, J. Liu, and J. Hu (2002)
J. Biol. Chem. 277, 23764-23772
   Abstract »    Full Text »    PDF »
An expanded view of bacterial DNA replication.
M.-F. Noirot-Gros, E. Dervyn, L. J. Wu, P. Mervelet, J. Errington, S. D. Ehrlich, and P. Noirot (2002)
PNAS 99, 8342-8347
   Abstract »    Full Text »    PDF »
The Axin-like protein PRY-1 is a negative regulator of a canonical Wnt pathway in C. elegans.
H. C. Korswagen, D. Y.M. Coudreuse, M. C. Betist, S. van de Water, D. Zivkovic, and H. C. Clevers (2002)
Genes & Dev. 16, 1291-1302
   Abstract »    Full Text »    PDF »
Elucidation of an Archaeal Replication Protein Network to Generate Enhanced PCR Enzymes.
M. Motz, I. Kober, C. Girardot, E. Loeser, U. Bauer, M. Albers, G. Moeckel, E. Minch, H. Voss, C. Kilger, et al. (2002)
J. Biol. Chem. 277, 16179-16188
   Abstract »    Full Text »    PDF »
Protein Interactions: Two Methods for Assessment of the Reliability of High Throughput Observations.
C. M. Deane, L. Salwinski, I. Xenarios, and D. Eisenberg (2002)
Mol. Cell. Proteomics 1, 349-356
   Abstract »    Full Text »    PDF »
The Fission Yeast ES2 Homologue, Bis1, Interacts with the Ish1 Stress-responsive Nuclear Envelope Protein.
L. Taricani, M. L. Tejada, and P. G. Young (2002)
J. Biol. Chem. 277, 10562-10572
   Abstract »    Full Text »    PDF »
Interaction generality, a measurement to assess the reliability of a protein-protein interaction.
R. Saito, H. Suzuki, and Y. Hayashizaki (2002)
Nucleic Acids Res. 30, 1163-1168
   Abstract »    Full Text »    PDF »
Analysis of Mammalian Peroxin Interactions Using a Non-transcription-based Bacterial Two-hybrid Assay.
M. Fransen, C. Brees, K. Ghys, L. Amery, G. P. Mannaerts, D. Ladant, and P. P. Van Veldhoven (2002)
Mol. Cell. Proteomics 1, 243-252
   Abstract »    Full Text »    PDF »
Combined Functional Genomic Maps of the C. elegans DNA Damage Response.
S. J. Boulton, A. Gartner, J. Reboul, P. Vaglio, N. Dyson, D. E. Hill, and M. Vidal (2002)
Science 295, 127-131
   Abstract »    Full Text »    PDF »
DIP, the Database of Interacting Proteins: a research tool for studying cellular networks of protein interactions.
I. Xenarios, L. Salwinski, X. J. Duan, P. Higney, S.-M. Kim, and D. Eisenberg (2002)
Nucleic Acids Res. 30, 303-305
   Abstract »    Full Text »    PDF »
Identification of Potential Interaction Networks Using Sequence-Based Searches for Conserved Protein-Protein Interactions or ""Interologs"".
L. R. Matthews, P. Vaglio, J. Reboul, H. Ge, B. P. Davis, J. Garrels, S. Vincent, and M. Vidal (2001)
Genome Res. 11, 2120-2126
   Abstract »    Full Text »    PDF »
Role of C. elegans lin-40 MTA in vulval fate specification and morphogenesis.
Z. Chen and M. Han (2001)
Development 128, 4911-4921
   Abstract »    Full Text »    PDF »
Networking proteins in yeast.
T. R. Hazbun and S. Fields (2001)
PNAS 98, 4277-4278
   Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)