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Science 10 December 1999:
Vol. 286. no. 5447, pp. 2151 - 2153
DOI: 10.1126/science.286.5447.2151
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Reports

Three-Dimensional Structures of the TAFII-Containing Complexes TFIID and TFTC

Marjorie Brand, * Claire Leurent, * Véronique Mallouh, Làszlò Tora, Patrick Schultz dagger

TBP (TATA-binding protein)-associated factors (TAFIIs) are components of large multiprotein complexes such as TFIID, TFTC, STAGA, PCAF/GCN5, and SAGA, which play a key role in the regulation of gene expression by RNA polymerase II. The structures of TFIID and TFTC have been determined at 3.5-nanometer resolution by electron microscopy and digital image analysis of single particles. Human TFIID resembles a macromolecular clamp that contains four globular domains organized around a solvent-accessible groove of a size suitable to bind DNA. TFTC is larger and contains five domains, four of which are similar to TFIID.

Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/Université Louis Pasteur, Boite Postale 163, F-67404 Illkirch cedex, Communauté Urbaine de Strasbourg, France.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: pat{at}wotan.u-strasbg.fr

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Science. ISSN 0036-8075 (print), 1095-9203 (online)