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Science 12 November 1999:
Vol. 286. no. 5443, pp. 1351 - 1353
DOI: 10.1126/science.286.5443.1351
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Reports

A Genetic Map and Recombination Parameters of the Human Malaria Parasite Plasmodium falciparum

Xin-zhuan Su, 1 Michael T. Ferdig, 1 Yaming Huang, 2 Chuong Q. Huynh, 3 Anna Liu, 1 Jingtao You, 1 John C. Wootton, 3 Thomas E. Wellems 1*

Genetic investigations of malaria require a genome-wide, high-resolution linkage map of Plasmodium falciparum. A genetic cross was used to construct such a map from 901 markers that fall into 14 inferred linkage groups corresponding to the 14 nuclear chromosomes. Meiotic crossover activity in the genome proved high (17 kilobases per centimorgan) and notably uniform over chromosome length. Gene conversion events and spontaneous microsatellite length changes were evident in the inheritance data. The markers, map, and recombination parameters are facilitating genome sequence assembly, localization of determinants for such traits as virulence and drug resistance, and genetic studies of parasite field populations.

1 Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0425, USA.
2 Guangxi Institute of Parasitic Disease Control, 80 Taoyuan Road, Nanning, Guangxi 530021, Peoples' Republic of China.
3 Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894-6075, USA.
*   To whom correspondence should be addressed. E-mail: tew{at}helix.nih.gov

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