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Science 5 November 1999:
Vol. 286. no. 5442, pp. 1172 - 1174
DOI: 10.1126/science.286.5442.1172
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Focal Adhesion Motility Revealed in Stationary Fibroblasts

Lubomir B. Smilenov, *dagger Alexei Mikhailov, ddagger Robert J. Pelham Jr., Eugene E. Marcantonio, Gregg G. Gundersen §

Focal adhesions (FAs) are clustered integrins and associated proteins that mediate cell adhesion and signaling. A green fluorescent protein-beta 1 integrin chimera was used to label FAs in living cells. In stationary cells, FAs were highly motile, moving linearly for several plaque lengths toward the cell center. FA motility was independent of cell density and resulted from contraction of associated actin fibers. In migrating cells, FAs were stationary and only moved in the tail. FA motility in stationary cells suggests that cell movement may be regulated by a clutch-like mechanism by which the affinity of integrins to substrate may be altered in response to migratory cues.

Departments of Pathology and Anatomy and Cell Biology, Columbia University, New York, NY 10032, USA.
*   All authors contributed equally to this study.

dagger    Present address: Center for Radiological Research, Columbia University, New York, NY 10032, USA.

ddagger    Present address: Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.

§   To whom correspondence should be addressed. E-mail: ggg1{at}columbia.edu

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