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Science 5 November 1999:
Vol. 286. no. 5442, pp. 1146 - 1149
DOI: 10.1126/science.286.5442.1146
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Reports

An Adenosine Deaminase that Generates Inosine at the Wobble Position of tRNAs

André P. Gerber, Walter Keller *

Several transfer RNAs (tRNAs) contain inosine (I) at the first position of their anticodon (position 34); this modification is thought to enlarge the codon recognition capacity during protein synthesis. The tRNA-specific adenosine deaminase of Saccharomyces cerevisiae that forms I34 in tRNAs is described. The heterodimeric enzyme consists of two sequence-related subunits (Tad2p/ADAT2 and Tad3p/ADAT3), both of which contain cytidine deaminase (CDA) motifs. Each subunit is encoded by an essential gene (TAD2 and TAD3), indicating that I34 is an indispensable base modification in elongating tRNAs. These results provide an evolutionary link between the CDA superfamily and RNA-dependent adenosine deaminases (ADARs/ADATs).

Department of Cell Biology, Biozentrum, University of Basel, 4056 Basel, Switzerland.
*   To whom correspondence should be addressed. E-mail: walter.keller{at}unibas.ch

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