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Science 24 September 1999:
Vol. 285. no. 5436, pp. 2133 - 2135
DOI: 10.1126/science.285.5436.2133
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Reports
Identification of an RNA-Protein Bridge Spanning the Ribosomal Subunit Interface
Gloria M. Culver,
*
Jamie H. Cate,
G. Zh. Yusupova,
Marat M. Yusupov,
Harry
F. Noller
The 7.8 angstrom crystal structure of the 70S
ribosome reveals a discrete double-helical bridge (B4) that projects
from the 50S subunit, making contact with the 30S
subunit. Preliminary modeling studies localized its contact site, near
the bottom of the platform, to the binding site for ribosomal protein
S15. Directed hydroxyl radical probing from iron(II) tethered to S15
specifically cleaved nucleotides in the 715 loop of domain II of
23S ribosomal RNA, one of the known sites in 23S
ribosomal RNA that are footprinted by the 30S subunit.
Reconstitution studies show that protection of the 715 loop, but none
of the other 30S-dependent protections, is correlated with
the presence of S15 in the 30S subunit. The 715 loop is
specifically protected by binding free S15 to 50S subunits.
Moreover, the previously determined structure of a homologous stem-loop
from U2 small nuclear RNA fits closely to the electron density of the
bridge.
Center for Molecular Biology of RNA, Sinsheimer Laboratories,
University of California, Santa Cruz, CA 95064, USA.
*
Present address: Department of Biochemistry, Biophysics, and Molecular
Biology, Iowa State University, Ames, IA 50011, USA.
Present address: Whitehead Institute for Biomedical Research
and Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
To whom correspondence should be addressed.
Read the Full Text
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