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Science 24 September 1999:
Vol. 285. no. 5436, pp. 2095 - 2104
DOI: 10.1126/science.285.5436.2095
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Research Articles

X-ray Crystal Structures of 70S Ribosome Functional Complexes

Jamie H. Cate, 1*dagger ddagger Marat M. Yusupov, 1*ddagger Gulnara Zh. Yusupova, 1 Thomas N. Earnest, 2 Harry F. Noller 1*

Structures of 70S ribosome complexes containing messenger RNA and transfer RNA (tRNA), or tRNA analogs, have been solved by x-ray crystallography at up to 7.8 angstrom resolution. Many details of the interactions between tRNA and the ribosome, and of the packing arrangements of ribosomal RNA (rRNA) helices in and between the ribosomal subunits, can be seen. Numerous contacts are made between the 30S subunit and the P-tRNA anticodon stem-loop; in contrast, the anticodon region of A-tRNA is much more exposed. A complex network of molecular interactions suggestive of a functional relay is centered around the long penultimate stem of 16S rRNA at the subunit interface, including interactions involving the "switch" helix and decoding site of 16S rRNA, and RNA bridges from the 50S subunit.

1 Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA.
2 Macromolecular Crystallography Facility, Advanced Light Source, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
*   To whom correspondence should be addressed. E-mail: cate{at}wi.mit.edu (J.H.C), marat{at}darwin.ucsc.edu (M.M.Y.), and harry{at}nuvolari.ucsc.edu (H.F.N.)

dagger    Present address: Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, MA 02142, USA.

ddagger    These authors contributed equally to this work.

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Science. ISSN 0036-8075 (print), 1095-9203 (online)