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Science 13 August 1999:
Vol. 285. no. 5430, pp. 1080 - 1084
DOI: 10.1126/science.285.5430.1080
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Reports

Coordinate Regulation of RAG1 and RAG2 by Cell Type-Specific DNA Elements 5' of RAG2

Wong Yu, 1 Ziva Misulovin, 2 Heikyung Suh, 2 Richard R. Hardy, 3 Mila Jankovic, 1 Nikos Yannoutsos, 1 Michel C. Nussenzweig 2*

RAG1 and RAG2 are essential for V(D)J recombination and lymphocyte development. These genes are thought to encode a transposase derived from a mobile genetic element that was inserted into the vertebrate genome 450 million years ago. The regulation of RAG1 and RAG2 was investigated in vivo with bacterial artificial chromosome (BAC) transgenes containing a fluorescent indicator. Coordinate expression of RAG1 and RAG2 in B and T cells was found to be regulated by distinct genetic elements found on the 5' side of the RAG2 gene. This observation suggests a mechanism by which asymmetrically disposed cis DNA elements could influence the expression of the primordial transposon and thereby capture RAGs for vertebrate evolution.

1 Laboratory of Molecular Immunology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
2 Howard Hughes Medical Institute, Laboratory of Molecular Immunology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
3 Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
*   To whom correspondence should be addressed. E-mail: nussen{at}rockvax.rockefeller.edu

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