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Science 30 July 1999:
Vol. 285. no. 5428, pp. 754 - 756
DOI: 10.1126/science.285.5428.754
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Reports

Embryonic Stem Cell-Derived Glial Precursors: A Source of Myelinating Transplants

Oliver Brüstle, 1* Kimberly N. Jones, 12 Randall D. Learish, 3dagger Khalad Karram, 1 Khalid Choudhary, 12 Otmar D. Wiestler, 1 Ian D. Duncan, 3 Ronald D. G. McKay 2

Self-renewing, totipotent embryonic stem (ES) cells may provide a virtually unlimited donor source for transplantation. A protocol that permits the in vitro generation of precursors for oligodendrocytes and astrocytes from ES cells was devised. Transplantation in a rat model of a human myelin disease shows that these ES cell-derived precursors interact with host neurons and efficiently myelinate axons in brain and spinal cord. Thus, ES cells can serve as a valuable source of cell type-specific somatic precursors for neural transplantation.

1 Department of Neuropathology, University of Bonn Medical Center, Sigmund-Freud-Straße 25, 53105 Bonn, Germany.
2 Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-4092, USA.
3 Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA.
*   To whom correspondence should be addressed. E-mail: brustle{at}uni-bonn.de

dagger    Present address: Promega Corp., 2800 Woods Hollow Road, Madison, WI 53711, USA.

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