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Science 21 May 1999:
Vol. 284. no. 5418, pp. 1359 - 1362
DOI: 10.1126/science.284.5418.1359
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Reports

Stimulation of Microtubule Aster Formation and Spindle Assembly by the Small GTPase Ran

Andrew Wilde, Yixian Zheng *

Ran, a small guanosine triphosphatase, is suggested to have additional functions beyond its well-characterized role in nuclear trafficking. Guanosine triphosphate-bound Ran, but not guanosine diphosphate-bound Ran, stimulated polymerization of astral microtubules from centrosomes assembled on Xenopus sperm. Moreover, a Ran allele with a mutation in the effector domain (RanL43E) induced the formation of microtubule asters and spindle assembly, in the absence of sperm nuclei, in a gamma TuRC (gamma -tubulin ring complex)- and XMAP215 (Xenopus microtubule associated protein)-dependent manner. Therefore, Ran could be a key signaling molecule regulating microtubule polymerization during mitosis.

Carnegie Institution of Washington, Department of Embryology, Baltimore, MD 21210, USA.
*   To whom correspondence should be addressed. E-mail: zheng{at}mail1.ciwemb.edu

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