Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 5 March 1999:
Vol. 283. no. 5407, pp. 1535 - 1538
DOI: 10.1126/science.283.5407.1535
Prev | Table of Contents | Next

Reports

Adhesive and Mammalian Transglutaminase Substrate Properties of Candida albicans Hwp1

Janet F. Staab, 1 Steven D. Bradway, 2* Paul L. Fidel, 3 Paula Sundstrom 1dagger

The pathogenesis of candidiasis involves invasion of host tissues by filamentous forms of the opportunistic yeast Candida albicans. Morphology-specific gene products may confer proinvasive properties. A hypha-specific surface protein, Hwp1, with similarities to mammalian small proline-rich proteins was shown to serve as a substrate for mammalian transglutaminases. Candida albicans strains lacking Hwp1 were unable to form stable attachments to human buccal epithelial cells and had a reduced capacity to cause systemic candidiasis in mice. This represents a paradigm for microbial adhesion that implicates essential host enzymes.

1 Department of Medical Microbiology and Immunology, College of Medicine and Public Health,
2 Department of Periodontology, College of Dentistry, Ohio State University, 333 West Tenth Avenue, Columbus, OH 43210, USA.
3 Department of Microbiology, Immunology, and Parasitology, Louisiana State University Medical Center, 1901 Perdido Street, New Orleans, LA 70112, USA.
*   Present address: Grays Harbor Dental Specialists, 105 South Broadway, Aberdeen, WA 98520, USA.

dagger    To whom correspondence should be addressed. E-mail:sundstrom.1{at}osu.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Distinct domains of the Candida albicans adhesin Eap1p mediate cell-cell and cell-substrate interactions.
F. Li and S. P. Palecek (2008)
Microbiology 154, 1193-1203
   Abstract »    Full Text »    PDF »
Gene Overexpression/Suppression Analysis of Candidate Virulence Factors of Candida albicans.
Y. Fu, G. Luo, B. J. Spellberg, J. E. Edwards Jr., and A. S. Ibrahim (2008)
Eukaryot. Cell 7, 483-492
   Abstract »    Full Text »    PDF »
Hypoxic conditions and iron restriction affect the cell-wall proteome of Candida albicans grown under vagina-simulative conditions.
G. J. Sosinska, P. W. J. de Groot, M. J. Teixeira de Mattos, H. L. Dekker, C. G. de Koster, K. J. Hellingwerf, and F. M. Klis (2008)
Microbiology 154, 510-520
   Abstract »    Full Text »    PDF »
Genome-Wide Transcriptional Profiling of the Cyclic AMP-Dependent Signaling Pathway during Morphogenic Transitions of Candida albicans.
Y.-S. Bahn, M. Molenda, J. F. Staab, C. A. Lyman, L. J. Gordon, and P. Sundstrom (2007)
Eukaryot. Cell 6, 2376-2390
   Abstract »    Full Text »    PDF »
Saliva: a Dynamic Proteome.
E.J. Helmerhorst and F.G. Oppenheim (2007)
J. Dent. Res. 86, 680-693
   Abstract »    Full Text »    PDF »
A Biochemical Guide to Yeast Adhesins: Glycoproteins for Social and Antisocial Occasions.
A. M. Dranginis, J. M. Rauceo, J. E. Coronado, and P. N. Lipke (2007)
Microbiol. Mol. Biol. Rev. 71, 282-294
   Abstract »    Full Text »    PDF »
Environmental Sensing and Signal Transduction Pathways Regulating Morphopathogenic Determinants of Candida albicans.
S. Biswas, P. Van Dijck, and A. Datta (2007)
Microbiol. Mol. Biol. Rev. 71, 348-376
   Abstract »    Full Text »    PDF »
Eap1p, an Adhesin That Mediates Candida albicans Biofilm Formation In Vitro and In Vivo.
F. Li, M. J. Svarovsky, A. J. Karlsson, J. P. Wagner, K. Marchillo, P. Oshel, D. Andes, and S. P. Palecek (2007)
Eukaryot. Cell 6, 931-939
   Abstract »    Full Text »    PDF »
Developmental Regulation of an Adhesin Gene during Cellular Morphogenesis in the Fungal Pathogen Candida albicans.
S. Argimon, J. A. Wishart, R. Leng, S. Macaskill, A. Mavor, T. Alexandris, S. Nicholls, A. W. Knight, B. Enjalbert, R. Walmsley, et al. (2007)
Eukaryot. Cell 6, 682-692
   Abstract »    Full Text »    PDF »
A 368-Base-Pair cis-Acting HWP1 Promoter Region, HCR, of Candida albicans Confers Hypha-Specific Gene Regulation and Binds Architectural Transcription Factors Nhp6 and Gcf1p.
S. Kim, M. J. Wolyniak, J. F. Staab, and P. Sundstrom (2007)
Eukaryot. Cell 6, 693-709
   Abstract »    Full Text »    PDF »
Biofilm formation by fluconazole-resistant Candida albicans strains is inhibited by fluconazole.
I. Bruzual, P. Riggle, S. Hadley, and C. A. Kumamoto (2007)
J. Antimicrob. Chemother. 59, 441-450
   Abstract »    Full Text »    PDF »
Comprehensive Analysis of Glycosylphosphatidylinositol-Anchored Proteins in Candida albicans.
M. L. Richard and A. Plaine (2007)
Eukaryot. Cell 6, 119-133
   Full Text »    PDF »
Function of Candida albicans Adhesin Hwp1 in Biofilm Formation..
C. J. Nobile, J. E. Nett, D. R. Andes, and A. P. Mitchell (2006)
Eukaryot. Cell 5, 1604-1610
   Abstract »    Full Text »    PDF »
Candida albicans HWP1 gene expression and host antibody responses in colonization and disease..
J. R. Naglik, F. Fostira, J. Ruprai, J. F. Staab, S. J. Challacombe, and P. Sundstrom (2006)
J. Med. Microbiol. 55, 1323-1327
   Abstract »    Full Text »    PDF »
Amplification of the Hyphal Wall Protein 1 Gene To Distinguish Candida albicans from Candida dubliniensis..
O. Romeo, C. Racco, and G. Criseo (2006)
J. Clin. Microbiol. 44, 2590-2592
   Abstract »    Full Text »    PDF »
Identification and Characterization of Cor33p, a Novel Protein Implicated in Tolerance towards Oxidative Stress in Candida albicans.
K. Sohn, M. Roehm, C. Urban, N. Saunders, D. Rothenstein, F. Lottspeich, K. Schroppel, H. Brunner, and S. Rupp (2005)
Eukaryot. Cell 4, 2160-2169
   Abstract »    Full Text »    PDF »
Candida albicans IRS4 contributes to hyphal formation and virulence after the initial stages of disseminated candidiasis.
H. Badrane, S. Cheng, M. H. Nguyen, H. Y. Jia, Z. Zhang, N. Weisner, and C. J. Clancy (2005)
Microbiology 151, 2923-2931
   Abstract »    Full Text »    PDF »
Virulence of the Fungal Pathogen Candida albicans Requires the Five Isoforms of Protein Mannosyltransferases.
M. Rouabhia, M. Schaller, C. Corbucci, A. Vecchiarelli, S. K.-H. Prill, L. Giasson, and J. F. Ernst (2005)
Infect. Immun. 73, 4571-4580
   Abstract »    Full Text »    PDF »
Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection.
C. C. Villar, H. Kashleva, A. P. Mitchell, and A. Dongari-Bagtzoglou (2005)
Infect. Immun. 73, 4588-4595
   Abstract »    Full Text »    PDF »
Efficacy of the Antiadhesin Octyl O-(2-Acetamido-2-Deoxy-{beta}-D- Galactopyranosyl)-(1-4)-2-O-Propyl-{beta}-D-Galactopyranoside (Fimbrigal-P) in a Rat Oral Candidiasis Model.
M. Foldvari, M. R. Jaafari, J. Radhi, and D. Segal (2005)
Antimicrob. Agents Chemother. 49, 2887-2894
   Abstract »    Full Text »    PDF »
Induction of the Candida albicans Filamentous Growth Program by Relief of Transcriptional Repression: A Genome-wide Analysis.
D. Kadosh and A. D. Johnson (2005)
Mol. Biol. Cell 16, 2903-2912
   Abstract »    Full Text »    PDF »
Flanking direct repeats of hisG alter URA3 marker expression at the HWP1 locus of Candida albicans.
L. L. Sharkey, W.-l. Liao, A. K. Ghosh, and W. A. Fonzi (2005)
Microbiology 151, 1061-1071
   Abstract »    Full Text »    PDF »
Regulation of the Cdc42/Cdc24 GTPase Module during Candida albicans Hyphal Growth.
M. Bassilana, J. Hopkins, and R. A. Arkowitz (2005)
Eukaryot. Cell 4, 588-603
   Abstract »    Full Text »    PDF »
KRE5 Gene Null Mutant Strains of Candida albicans Are Avirulent and Have Altered Cell Wall Composition and Hypha Formation Properties.
A. B. Herrero, P. Magnelli, M. K. Mansour, S. M. Levitz, H. Bussey, and C. Abeijon (2004)
Eukaryot. Cell 3, 1423-1432
   Abstract »    Full Text »    PDF »
Deficiencies in the essential Smp3 mannosyltransferase block glycosylphosphatidylinositol assembly and lead to defects in growth and cell wall biogenesis in Candida albicans.
S. J. Grimme, P. A. Colussi, C. H. Taron, and P. Orlean (2004)
Microbiology 150, 3115-3128
   Abstract »    Full Text »    PDF »
Systematic identification in silico of covalently bound cell wall proteins and analysis of protein-polysaccharide linkages of the human pathogen Candida glabrata.
M. Weig, L. Jansch, U. Gross, C. G. De Koster, F. M. Klis, and P. W. J. De Groot (2004)
Microbiology 150, 3129-3144
   Abstract »    Full Text »    PDF »
The GPI-anchored protein CaEcm33p is required for cell wall integrity, morphogenesis and virulence in Candida albicans.
R. Martinez-Lopez, L. Monteoliva, R. Diez-Orejas, C. Nombela, and C. Gil (2004)
Microbiology 150, 3341-3354
   Abstract »    Full Text »    PDF »
Comparative genomics using Candida albicans DNA microarrays reveals absence and divergence of virulence-associated genes in Candida dubliniensis.
G. Moran, C. Stokes, S. Thewes, B. Hube, D. C. Coleman, and D. Sullivan (2004)
Microbiology 150, 3363-3382
   Abstract »    Full Text »    PDF »
Expression of Transglutaminase Substrate Activity on Candida albicans Germ Tubes through a Coiled, Disulfide-bonded N-terminal Domain of Hwp1 Requires C-terminal Glycosylphosphatidylinositol Modification.
J. F. Staab, Y.-S. Bahn, C.-H. Tai, P. F. Cook, and P. Sundstrom (2004)
J. Biol. Chem. 279, 40737-40747
   Abstract »    Full Text »    PDF »
Global Cell Surface Conformational Shift Mediated by a Candida albicans Adhesin.
J. M. Rauceo, N. K. Gaur, K.-G. Lee, J. E. Edwards, S. A. Klotz, and P. N. Lipke (2004)
Infect. Immun. 72, 4948-4955
   Abstract »    Full Text »    PDF »
Ectopic Expression of URA3 Can Influence the Virulence Phenotypes and Proteome of Candida albicans but Can Be Overcome by Targeted Reintegration of URA3 at the RPS10 Locus.
A. Brand, D. M. MacCallum, A. J. P. Brown, N. A. R. Gow, and F. C. Odds (2004)
Eukaryot. Cell 3, 900-909
   Abstract »    Full Text »    PDF »
Proteomic Analysis of Candida albicans Cell Walls Reveals Covalently Bound Carbohydrate-Active Enzymes and Adhesins.
P. W. J. de Groot, A. D. de Boer, J. Cunningham, H. L. Dekker, L. de Jong, K. J. Hellingwerf, C. de Koster, and F. M. Klis (2004)
Eukaryot. Cell 3, 955-965
   Abstract »    Full Text »    PDF »
Functional and Structural Diversity in the Als Protein Family of Candida albicans.
D. C. Sheppard, M. R. Yeaman, W. H. Welch, Q. T. Phan, Y. Fu, A. S. Ibrahim, S. G. Filler, M. Zhang, A. J. Waring, and J. E. Edwards Jr. (2004)
J. Biol. Chem. 279, 30480-30489
   Abstract »    Full Text »    PDF »
Positive Directional Selection in the Proline-Rich Antigen (PRA) Gene Among the Human Pathogenic Fungi Coccidioides immitis, C. posadasii and Their Closest Relatives.
H. Johannesson, P. Vidal, J. Guarro, R. A. Herr, G. T. Cole, and J. W. Taylor (2004)
Mol. Biol. Evol. 21, 1134-1145
   Abstract »    Full Text »    PDF »
Degenerate Peptide Recognition by Candida albicans Adhesins Als5p and Als1p.
S. A. Klotz, N. K. Gaur, D. F. Lake, V. Chan, J. Rauceo, and P. N. Lipke (2004)
Infect. Immun. 72, 2029-2034
   Abstract »    Full Text »    PDF »
Accessibility of the peptide backbone of protein ligands is a key specificity determinant in Candida albicans SRS adherence.
N. K. Gaur and S. A. Klotz (2004)
Microbiology 150, 277-284
   Abstract »    Full Text »    PDF »
EAP1, a Candida albicans Gene Involved in Binding Human Epithelial Cells.
F. Li and S. P. Palecek (2003)
Eukaryot. Cell 2, 1266-1273
   Abstract »    Full Text »    PDF »
The Adhesin Hwp1 and the First Daughter Cell Localize to the a/a Portion of the Conjugation Bridge during Candida albicans Mating.
K. J. Daniels, S. R. Lockhart, J. F. Staab, P. Sundstrom, and D. R. Soll (2003)
Mol. Biol. Cell 14, 4920-4930
   Abstract »    Full Text »    PDF »
Identification and Characterization of a Candida albicans Mating Pheromone.
R. J. Bennett, M. A. Uhl, M. G. Miller, and A. D. Johnson (2003)
Mol. Cell. Biol. 23, 8189-8201
   Abstract »    Full Text »    PDF »
{alpha}-Pheromone-Induced "Shmooing" and Gene Regulation Require White-Opaque Switching during Candida albicans Mating.
S. R. Lockhart, R. Zhao, K. J. Daniels, and D. R. Soll (2003)
Eukaryot. Cell 2, 847-855
   Abstract »    Full Text »    PDF »
Integrative, multifunctional plasmids for hypha-specific or constitutive expression of green fluorescent protein in Candida albicans.
J. F. Staab, Y.-S. Bahn, and P. Sundstrom (2003)
Microbiology 149, 2977-2986
   Abstract »    Full Text »    PDF »
Posttranslational Modifications Required for Cell Surface Localization and Function of the Fungal Adhesin Aga1p.
G. Huang, M. Zhang, and S. E. Erdman (2003)
Eukaryot. Cell 2, 1099-1114
   Abstract »    Full Text »    PDF »
Identification and study of a Candida albicans protein homologous to Saccharomyces cerevisiae Ssr1p, an internal cell-wall protein.
A. Garcera, A. I. Martinez, L. Castillo, M. V. Elorza, R. Sentandreu, and E. Valentin (2003)
Microbiology 149, 2137-2145
   Abstract »    Full Text »    PDF »
Differentiation of Candida albicans and Candida dubliniensis by Using Recombinant Human Antibody Single-Chain Variable Fragments Specific for Hyphae.
J. M. Bliss, M. A. Sullivan, J. Malone, and C. G. Haidaris (2003)
J. Clin. Microbiol. 41, 1152-1160
   Abstract »    Full Text »    PDF »
Salivary Biochemistry in Buffalo: The Legacy of Michael J. Levine.
F. A. Scannapieco (2003)
J. Dent. Res. 82, 76-81
   Full Text »    PDF »
Contribution of Candida albicans ALS1 to the Pathogenesis of Experimental Oropharyngeal Candidiasis.
Y. Kamai, M. Kubota, Y. Kamai, T. Hosokawa, T. Fukuoka, and S. G. Filler (2002)
Infect. Immun. 70, 5256-5258
   Abstract »    Full Text »    PDF »
Septin Function in Candida albicans Morphogenesis.
A. J. Warenda and J. B. Konopka (2002)
Mol. Biol. Cell 13, 2732-2746
   Abstract »    Full Text »    PDF »
A Parasitic Phase-Specific Adhesin of Coccidioides immitis Contributes to the Virulence of This Respiratory Fungal Pathogen.
C.-Y. Hung, J.-J. Yu, K. R. Seshan, U. Reichard, and G. T. Cole (2002)
Infect. Immun. 70, 3443-3456
   Abstract »    Full Text »    PDF »
Reevaluation of the Role of HWP1 in Systemic Candidiasis by Use of Candida albicans Strains with Selectable Marker URA3 Targeted to the ENO1 Locus.
P. Sundstrom, J. E. Cutler, and J. F. Staab (2002)
Infect. Immun. 70, 3281-3283
   Abstract »    Full Text »    PDF »
Roles of TUP1 in Switching, Phase Maintenance, and Phase-Specific Gene Expression in Candida albicans.
R. Zhao, S. R. Lockhart, K. Daniels, and D. R. Soll (2002)
Eukaryot. Cell 1, 353-365
   Abstract »    Full Text »    PDF »
Transglutaminase activity is involved in Saccharomyces cerevisiae wall construction.
M. Iranzo, C. Aguado, C. Pallotti, J. V. Canizares, and S. Mormeneo (2002)
Microbiology 148, 1329-1334
   Abstract »    Full Text »    PDF »
Disruption of the Candida albicans TPS2 Gene Encoding Trehalose-6-Phosphate Phosphatase Decreases Infectivity without Affecting Hypha Formation.
P. Van Dijck, L. De Rop, K. Szlufcik, E. Van Ael, and J. M. Thevelein (2002)
Infect. Immun. 70, 1772-1782
   Abstract »    Full Text »    PDF »
In vitro reconstructed human epithelia reveal contributions of Candida albicans EFG1 and CPH1 to adhesion and invasion.
C. Dieterich, M. Schandar, M. Noll, F.-J. Johannes, H. Brunner, T. Graeve, and S. Rupp (2002)
Microbiology 148, 497-506
   Abstract »    Full Text »    PDF »
The Basic Helix-Loop-Helix Transcription Factor Cph2 Regulates Hyphal Development in Candida albicans Partly via Tec1.
S. Lane, S. Zhou, T. Pan, Q. Dai, and H. Liu (2001)
Mol. Cell. Biol. 21, 6418-6428
   Abstract »    Full Text »    PDF »
Traversal of Candida albicans across Human Blood-Brain Barrier In Vitro.
A. Y. Jong, M. F. Stins, S.-H. Huang, S. H. M. Chen, and K. S. Kim (2001)
Infect. Immun. 69, 4536-4544
   Abstract »    Full Text »    PDF »
Overexpression of a dominant-negative allele of YPT1 inhibits growth and aspartyl protease secretion in Candida albicans.
S. A. Lee, Y. Mao, Z. Zhang, and B. Wong (2001)
Microbiology 147, 1961-1970
   Abstract »    Full Text »    PDF »
Deficiency of D-Erythroascorbic Acid Attenuates Hyphal Growth and Virulence of Candida albicans.
W.-K. Huh, S.-T. Kim, H. Kim, G. Jeong, and S.-O. Kang (2001)
Infect. Immun. 69, 3939-3946
   Abstract »    Full Text »    PDF »
Tagging Morphogenetic Genes by Insertional Mutagenesis in the Yeast Yarrowia lipolytica.
M. Richard, R. R. Quijano, S. Bezzate, F. Bordon-Pallier, and C. Gaillardin (2001)
J. Bacteriol. 183, 3098-3107
   Abstract »    Full Text »
CAP1, an Adenylate Cyclase-Associated Protein Gene, Regulates Bud-Hypha Transitions, Filamentous Growth, and Cyclic AMP Levels and Is Required for Virulence of Candida albicans.
Y.-S. Bahn and P. Sundstrom (2001)
J. Bacteriol. 183, 3211-3223
   Abstract »    Full Text »
Rfg1, a Protein Related to the Saccharomyces cerevisiae Hypoxic Regulator Rox1, Controls Filamentous Growth and Virulence in Candida albicans.
D. Kadosh and A. D. Johnson (2001)
Mol. Cell. Biol. 21, 2496-2505
   Abstract »    Full Text »
A Single-Transformation Gene Function Test in Diploid Candida albicans.
B. Enloe, A. Diamond, and A. P. Mitchell (2000)
J. Bacteriol. 182, 5730-5736
   Abstract »    Full Text »
Identification and Characterization of TUP1-Regulated Genes in Candida albicans.
B. R. Braun, W. S. Head, M. X. Wang, and A. D. Johnson (2000)
Genetics 156, 31-44
   Abstract »    Full Text »
Transcription factors in Candida albicans - environmental control of morphogenesis.
J. F. Ernst (2000)
Microbiology 146, 1763-1774
   Full Text »
Dominant Active Alleles of RIM101 (PRR2) Bypass the pH Restriction on Filamentation of Candida albicans.
A. El Barkani, O. Kurzai, W. A. Fonzi, A. Ramon, A. Porta, M. Frosch, and F. A. Mühlschlegel (2000)
Mol. Cell. Biol. 20, 4635-4647
   Abstract »    Full Text »
Reduced Virulence of HWP1-Deficient Mutants of Candida albicans and Their Interactions with Host Cells.
N. Tsuchimori, L. L. Sharkey, W. A. Fonzi, S. W. French, J. E. Edwards Jr., and S. G. Filler (2000)
Infect. Immun. 68, 1997-2002
   Abstract »    Full Text »    PDF »
Overexpression of the Candida albicans ALA1 Gene in Saccharomyces cerevisiae Results in Aggregation following Attachment of Yeast Cells to Extracellular Matrix Proteins, Adherence Properties Similar to Those of Candida albicans.
N. K. Gaur, S. A. Klotz, and R. L. Henderson (1999)
Infect. Immun. 67, 6040-6047
   Abstract »    Full Text »    PDF »
HWP1 Functions in the Morphological Development of Candida albicans Downstream of EFG1, TUP1, and RBF1.
L. L. Sharkey, M. D. McNemar, S. M. Saporito-Irwin, P. S. Sypherd, and W. A. Fonzi (1999)
J. Bacteriol. 181, 5273-5279
   Abstract »    Full Text »
An Adhesin of the Yeast Pathogen Candida glabrata Mediating Adherence to Human Epithelial Cells.
B. P. Cormack, N. Ghori, and S. Falkow (1999)
Science 285, 578-582
   Abstract »    Full Text »
DNA Array Studies Demonstrate Convergent Regulation of Virulence Factors by Cph1, Cph2, and Efg1 in Candida albicans.
S. Lane, C. Birse, S. Zhou, R. Matson, and H. Liu (2001)
J. Biol. Chem. 276, 48988-48996
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)