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Science 12 February 1999:
Vol. 283. no. 5404, pp. 1001 - 1004
DOI: 10.1126/science.283.5404.1001
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Reports

Efficient Bypass of a Thymine-Thymine Dimer by Yeast DNA Polymerase, Pol&eegr;

Robert E. Johnson, Satya Prakash, Louise Prakash *

The RAD30 gene of the yeast Saccharomyces cerevisiae is required for the error-free postreplicational repair of DNA that has been damaged by ultraviolet irradiation. Here, RAD30 is shown to encode a DNA polymerase that can replicate efficiently past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally blocks DNA polymerases. When incubated in vitro with all four nucleotides, Rad30 incorporates two adenines opposite the thymine-thymine dimer. Rad30 is the seventh eukaryotic DNA polymerase to be described and hence is named DNA polymerase eta .

Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, 6.104 Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061, USA.
*   To whom correspondence should be addressed. E-mail: lprakash{at}scms.utmb.edu

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S. Prakash and L. Prakash (2002)
Genes & Dev. 16, 1872-1883
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DNA damage responses protect xeroderma pigmentosum variant from UVC-induced clastogenesis.
M. Cordeiro-Stone, A. Frank, M. Bryant, I. Oguejiofor, S. B. Hatch, L. D. McDaniel, and W. K. Kaufmann (2002)
Carcinogenesis 23, 959-966
   Abstract »    Full Text »    PDF »
Dynamics of Gapped DNA Recognition by Human Polymerase beta.
M. J. Jezewska, R. Galletto, and W. Bujalowski (2002)
J. Biol. Chem. 277, 20316-20327
   Abstract »    Full Text »    PDF »
Low Fidelity DNA Synthesis by a Y Family DNA Polymerase Due to Misalignment in the Active Site.
R. J. Kokoska, K. Bebenek, F. Boudsocq, R. Woodgate, and T. A. Kunkel (2002)
J. Biol. Chem. 277, 19633-19638
   Abstract »    Full Text »    PDF »
Yeast Rev1 Protein Is a G Template-specific DNA Polymerase.
L. Haracska, S. Prakash, and L. Prakash (2002)
J. Biol. Chem. 277, 15546-15551
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Requirement of RAD5 and MMS2 for Postreplication Repair of UV-Damaged DNA in Saccharomyces cerevisiae.
C. A. Torres-Ramos, S. Prakash, and L. Prakash (2002)
Mol. Cell. Biol. 22, 2419-2426
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Response of human REV1 to different DNA damage: preferential dCMP insertion opposite the lesion.
Y. Zhang, X. Wu, O. Rechkoblit, N. E. Geacintov, J.-S. Taylor, and Z. Wang (2002)
Nucleic Acids Res. 30, 1630-1638
   Abstract »    Full Text »    PDF »
Preferential Misincorporation of Purine Nucleotides by Human DNA Polymerase eta Opposite Benzo[a]pyrene 7,8-Diol 9,10-Epoxide Deoxyguanosine Adducts.
D. Chiapperino, H. Kroth, I. H. Kramarczuk, J. M. Sayer, C. Masutani, F. Hanaoka, D. M. Jerina, and A. M. Cheh (2002)
J. Biol. Chem. 277, 11765-11771
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UV-induced T->C transition at a TT photoproduct site is dependent on Saccharomyces cerevisiae polymerase {eta}in vivo.
H. Zhang and W. Siede (2002)
Nucleic Acids Res. 30, 1262-1267
   Abstract »    Full Text »    PDF »
Stimulation of DNA Synthesis Activity of Human DNA Polymerase {kappa} by PCNA.
L. Haracska, I. Unk, R. E. Johnson, B. B. Phillips, J. Hurwitz, L. Prakash, and S. Prakash (2002)
Mol. Cell. Biol. 22, 784-791
   Abstract »    Full Text »    PDF »
Suppression of genetic defects within the RAD6 pathway by srs2 is specific for error-free post-replication repair but not for damage-induced mutagenesis.
S. Broomfield and W. Xiao (2002)
Nucleic Acids Res. 30, 732-739
   Abstract »    Full Text »    PDF »
Degradation or Maintenance: Actions of the Ubiquitin System on Eukaryotic Chromatin.
H. D. Ulrich (2002)
Eukaryot. Cell 1, 1-10
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