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Science 5 February 1999:
Vol. 283. no. 5403, pp. 836 - 839
DOI: 10.1126/science.283.5403.836
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Reports

Single-Channel Recording of a Store-Operated Ca2+ Channel in Jurkat T Lymphocytes

Hubert H. Kerschbaum, 1 Michael D. Cahalan 2*

In T lymphocytes, a store-operated calcium ion (Ca2+) entry mechanism termed the calcium release-activated Ca2+ channel (CRAC channel) underlies the sustained or oscillatory intracellular calcium concentration signal required for interleukin-2 gene expression and cell proliferation. The use of sodium ions as a current carrier enabled single-channel recordings of CRAC channels during activation, inactivation, and blockade of current in the presence of divalent cations. A large conductance of 36 to 40 picosiemens indicates that 100 to 400 CRAC channels are present in T lymphocytes.

1 Department of Animal Physiology, Institute of Zoology, University of Salzburg, A-5020 Salzburg, Austria.
2 Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA.
*   To whom correspondence should be addressed. E-mail: mcahalan{at}uci.edu

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