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Science 25 September 1998:
Vol. 281. no. 5385, pp. 2013 - 2016
DOI: 10.1126/science.281.5385.2013
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Reports

Semiconductor Nanocrystals as Fluorescent Biological Labels

Marcel Bruchez Jr., Mario Moronne, Peter Gin, Shimon Weiss, * A. Paul Alivisatos *

Semiconductor nanocrystals were prepared for use as fluorescent probes in biological staining and diagnostics. Compared with conventional fluorophores, the nanocrystals have a narrow, tunable, symmetric emission spectrum and are photochemically stable. The advantages of the broad, continuous excitation spectrum were demonstrated in a dual-emission, single-excitation labeling experiment on mouse fibroblasts. These nanocrystal probes are thus complementary and in some cases may be superior to existing fluorophores.

M. Bruchez Jr. and A. P. Alivisatos, Department of Chemistry, University of California, Berkeley, CA 94720, and Materials Sciences Division, Lawrence Berkeley National Laboratory (LBNL), 1 Cyclotron Road, Berkeley, CA 94720, USA. M. Moronne and P. Gin, Life Sciences Division, LBNL, 1 Cyclotron Road, Berkeley, CA 94720, USA. S. Weiss, Materials Sciences Division and Physical Biosciences Division. LBNL, 1 Cyclotron Road, Berkeley, CA 94720, USA.
*   To whom correspondence should be addressed. E-mail: sweiss{at}mhl.lbl.gov and alivis{at}uclink4.berkeley.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)