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Science 4 September 1998:
Vol. 281. no. 5382, pp. 1502 - 1505
DOI: 10.1126/science.281.5382.1502
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Reports

Segregation of Transcription and Replication Sites Into Higher Order Domains

Xiangyun Wei, Jagath Samarabandu, * Rakendu S. Devdhar, Alan J. Siegel, Raj Acharya, Ronald Berezney dagger

Microscopy shows that individual sites of DNA replication and transcription of mammalian nuclei segregate into sets of roughly 22 and 16 higher order domains, respectively. Each domain set displayed a distinct network-like appearance, including regions of individual domains and interdigitation of domains between the two networks. These data support a dynamic mosaic model for the higher order arrangement of genomic function inside the cell nuclei.

X. Wei, J. Samarabandu, A. J. Siegel, R. Berezney, Departments of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA. R. S. Devdhar and R. Acharya, Department of Electrical and Computer Engineering, State University of New York at Buffalo, Buffalo, NY 14260, USA.
*   Present address: Life Imaging Systems, 195 Dufferin Avenue, Suite 300, London, Ontario N6A1K7, Canada.

dagger    To whom correspondence should be addressed. E-mail: berezney{at}acsu.buffalo.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)