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Science 21 August 1998:
Vol. 281. no. 5380, pp. 1202 - 1206
DOI: 10.1126/science.281.5380.1202
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Reports

Characterization of an Ammonium Transport Protein from the Peribacteroid Membrane of Soybean Nodules

Brent N. Kaiser, Patrick M. Finnegan, Stephen D. Tyerman, Lynne F. Whitehead, Fraser J. Bergersen, David A. Day, * Michael K. Udvardi

Nitrogen-fixing bacteroids in legume root nodules are surrounded by the plant-derived peribacteroid membrane, which controls nutrient transfer between the symbionts. A nodule complementary DNA (GmSAT1) encoding an ammonium transporter has been isolated from soybean. GmSAT1 is preferentially transcribed in nodules and immunoblotting indicates that GmSAT1 is located on the peribacteroid membrane. [14C]methylammonium uptake and patch-clamp analysis of yeast expressing GmSAT1 demonstrated that it shares properties with a soybean peribacteroid membrane NH4+ channel described elsewhere. GmSAT1 is likely to be involved in the transfer of fixed nitrogen from the bacteroid to the host.

B. N. Kaiser, P. M. Finnegan, F. J. Bergersen, D. A. Day, M. K. Udvardi, Division of Biochemistry and Molecular Biology, The Australian National University, Canberra ACT 0200, Australia. S. D. Tyerman and L. F. Whitehead, Biology Department, Flinders, University of South Australia, GPO Box 2100, Adelaide 5001, Australia.
*   To whom correspondence should be addressed.

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